T/Ths @ 2:50 pm
M - P1-P7 (Banking Day)
T/Th - P1, P3, P5, P7
W/F - P2, P4, P6, P7
Day 1 Tuesday, Aug 27
In class:
What is Biotech?
Go over How to maintain a Scientific Notebook in Biotech
Lab Activity: Bleach Lab pgs 19-21 TB
HW:
Read Ch 1.1-1.2 and do review questions Ch 1.1-1.2 in ED - write questions and answers on paper with black ink
Read Cheese Lab before Thursday and bring whole milk and buttermilk on Thursday
Do Biotech Online, B.O., pg 13 Glo Fish
Day 2 Thursday, Aug 29
In class:
Complete and make final observations for Bleach Lab, lab report due on 9/7
Review How to Notebook and how to write an effective conclusion
Lab Activity: Cheese Lab
HW:
Read Ch 1.3 pgs 14-18 and do review questions 1.3 ED
Do Biotech Online (B.O.) pg 18 Genentech
Finish Bleach Lab report in Google Docs pgs 19-21 ED; lab report due on Th 9/7
*** Class only meets on Tuesdays and Thursdays
Day 1 Wednesday, August 21
In class:
Rules and Policies
Safety Rules
HW:
Sign up for Google Classroom, Quizizz, Edpuzzle
Study for Safety Test – Test on Thursday
Wear shoes to class – Always!
Day 2 Thursday, August 22
In class:
Safety Test
What is Biotechnology
HW:
Read Ch 1.1 Biotechnology ED (Ellyn Daugherty textbook)
Read Bleach Lab pgs 19-21 ED, Lab on Tuesday
Wear shoes, = please!
M - Labor Day Holiday
T/Th - P1, P3, P5, P7
W/F - P2, P4, P6, P7
Tuesday, Sept 2
In Class:
Review Scientific Method
Correct Ch 1.2-1.3 review questions
Lab Activity: Cheese Lab, make first observation, record in your notebook
Biotech Live: Activity 1 What is Biotechnology? pgs 32-33
Activity 2 Business Side pgs 33-34
HW:
Read ED Ch. 1.4 and review questions pgs 19-23 ED
Do B.O. Hot Jobs pg 26
Extra Credit: make cheese at home; DIY on foodwishes.com
Bleach Lab due Thurs 9/4; Did you send to intern to check for content/format?
Thursday, Sept 4
In Class:
Cheese Lab – Make 2nd observation, record in your notebook🡪 ready for cheese cloth?
Discuss Careers in Biotech
Do Biotech Live Activity 1.4 How Biotech Industry Improving Human Life? pg 35
Correct Ch 1.4 review questions
Bleach Lab due today– Did you send to intern to check for content/format?
HW:
Read ED Ch 1.5-1.6 and do review questions pgs 23-29 ED
Do B.O. Code of Business Conduct pg 29 ED
Read Summary and Lab Practices pgs 30-31
Ch 1 Test – Thurs 9/12
M/W – P1, P3, P5, P7
T/Th – P2, P4, P6, P7
Friday – Minimum Day
Tuesday, Sept 10
In class:
Review Ch 1.3-1.6 and correct review answers
Discuss careers and Biotethics in Biotechnology- watch video on Biochemist
Check cheeseball – make final observation: color, texture, smell
Go over Microscope Lab – due on Thurs, 9/12
Lab Activity: Organic Lab – lab report due on Thurs, 9/19
Homework:
Read Summary and Lab Practices on pgs 30-31 to review for Ch 1 Test
Study for Ch 1 Test – Test on Thurs, 9/12 (BRING CHROMEBOOK, charged)
Cheese Lab is due on Tues, 9/17
Read Ch 2.1, do review question
Remember to copy review questions and answer in complete sentences on a separate sheet
Thursday, Sept 12 (BTSN)
In class:
Ch 1 Test in class on Quizziz
Go over Ch 2.1 Review Questions, Organic Lab, and Microscope worksheet
Lab Activity: Organic Lab on Tuesday
Homework:
Read Ch 2.2-2.3, do review questions; remember to copy questions and answer in complete sentences
Complete cell structure and function worksheet
Finish Cheese Lab - due on Tues, 9/17
Read Organic Lab – lab on on Tues, 9/17
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Sept 17
In class:
Go over Organic Lab
Correct Ch 2.1-2.3 review questions
Due today: Cheese Lab, Microscope Lab (was due on Friday)
Lab Activity – Bacteria Lab: collect bacteria, streak media plate, test against antibiotics
Homework:
Read Ch 2.4 New Biotechnology and do 2.4 review questions
Do B.O. pgs 43 Kimchi, 46 Right Tool, 52 Molecular Models, and 82 Biotech Products
Finish Organic Lab - both are due on Thursday, 9/19
Ch 2 Test on Thursday, 9/26
Thursday, Sept 19
In class:
Due today: Organic Lab
Correct Ch 2.4 review questions
Go over Bacteria Lab and Gram Stain
Lab Activity: Continue Bacteria Lab; Gram Staining of bacteria
Homework:
Finish Bacteria Lab Report – due on Thursday, 9/26
Do Biotech Live Activity 2.1-2.3 pg 86
Read Ch 2 Summary and Lab Practices pg 81-86
Ch 2 Test on Thursday, 9/26
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Sept 24
In class:
Review Ch 2 The Raw Materials
Go over Bacteria Lab and Gram Stain
Lab Activity – Restreak bacteria with antibiotics; Grams Stain
Do SkillsUSA Certification Module
Homework:
Ch 2 Test on Thursday and Ch 2 packet due on Thursday
Read Ch 3.1-3.2 pgs 71-82 and do review questions
Do B.O. pg 74 on Metrics Conversion
Finish Bacteria Lab with antibiotics– due on Tuesday, 10/1
Gram Stain Lab – due on Thursday, 10/3
Thursday, Sept 26
In class:
Ch 2 Test and Ch 2 packet due today
Go over Ch 3.1-3.2 review questions and math problems
Go over Gram Stain Lab
Lab Activity – Pipetting Accuracy
Homework:
Read Ch 3.3-3.4 and do review questions
Do Math Problems
Bacteria Lab with antibiotics – due on Tuesday, 10/1
Gram Stain Lab – due on Thursday, 10/3
Pipetting Lab due Thursday, 10/3
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Oct 1
In class:
Correct all Ch 3.1 – 3.3 review questions and math problems
Go over Math Problems
Lab Activity – Pipetting Accuracy part 1 and 2
Due today: Bacteria Lab with antibiotics; Gram Stain Lab – due on Thurs, 10/3
Homework:
Read Ch 3.4 - 3.5 and do review questions
Do B.O. 150 Meter Homer pg 74, Positive Displacement pg 79,
Pipetting Accuracy Lab parts 1 and 2 – due on Tues, 10/8
Work on Math problems
Thursday, Oct 3
In class:
Go over Ch 3.5 review questions and math problems
Go over Pipetting Accuracy
Lab Activity – Micropippetting Exercise with colored dyes – due Thurs 10/10
Homework:
Read Ch 3.6 and do review questions
Read Summary and Lab Practices pgs 92-93
Pipetting Accuracy Lab parts 1 and 2 - due Tues, 10/8
Ch 3 Test – Thurs 10/10
Finish Math Problems
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Oct 8
In class:
Correct Ch 3.3-3.6 review questions and Math Problems
Ch 3 Test on Tues, 10/15
Continue Pipetting Accuracy Lab, part 2
Go over Math problems
Lab Activity: Micro-pipetting Lab with colored dyes – due Thurs, 10/17
Homework:
Study for Ch 3 Test – Test on Tues, 10/15
Finish Biotech Live – Activity 3.1-3.4 pgs 95-97 and Math problems
Pipetting Accuracy Lab, parts and 2 – due Tues, 10/15
Micro-pipetting Lab with colored dyes – due Thurs, 10/17
Thursday, Oct 10
In class:
Ch 3 Test
Continue with Biotech Live – Activity 3.5-3.6 pgs 98-100
Go over Math/MW of compounds
Activity: more practice on micro-pipetting
Homework:
Study for Ch 3 test - Test on Tues, 10/15
Read Ch 4.1 Studying DNA and do review questions
Pipetting Accuracy Lab, parts and 2 – due Tues, 10/15
Micro-pipetting Lab with colored dyes – due Thurs, 10/17
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7
F P1 - P7 Minimum Day
Tuesday, Oct 15
In class:
Go over Math Problems, Ch 3.5-3.6 review questions
Ch 4.1 DNA Structure
Lab Activity: Isolation of Strawberry DNA
Due today: Pipetting Accuracy parts 1 and 2
Homework:
Read Ch 4.2 Sources of DNA, do review questions
Do Biotech Online: Know your Genome pg 112, Baldness Gene pg 118
Download Amgen Lab Manual – Read ABE Lab 1 Gel Electrophoresis and do prelab notes
Strawberry DNA Lab – due on Thurs, 10/24
Ch 3 Test on Thurs, 10/17
Thursday, Oct 17
In class:
Ch 3 Test and Ch 3 packet due today
Ch 4.2 Sources of DNA, correct review questions
Activity: Cast a Gel for Gel Electrophoresis; make 1x SB buffer and 1% agarose gel;
- ABE Lab 1 – Gel Electrophoresis
Due today: Micropipetting Lab with red dye
Homework:
Read Ch 4.3 Isolating and Manipulating DNA
Do Biotech Online Recombinant Pharmaceuticals and Therapies, pgs 118-119
Read Lab 2 Amgen Lab Manual – pKan and pARA Digest with restriction enzymes
Strawberry DNA Lab – due on Thurs, 10/24
ABE Lab 1 – Gel Electrophoresis – due on Tues, 10/29
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Oct 22
In class:
Go over 4.3 DNA Structure and Isolation of DNA (strawberry)
Quiz on Ch 4.1-4.3
ABE Lab 1 Gel Electrophoresis
Lab Activity: ABE Lab 1 Gel Electrophoresis
ABE Lab 2 Restriction Digest of pKan and pAra
Homework:
Read Ch 4.4 Gel Electrophoresis and do review Questions
Read ABE Lab 3 Ligation
Finish ABE Lab 2,3 Prelab Notes
Do B.O. Chop and Go Electrophoresis pg 124
Do Ch 4 Biotech Live 4.1-4.2
Strawberry DNA Isolation Lab report – due on Thursday, Oct 24
Thursday, Oct 24
In class:
Go over Ch 4.4 Gel Electrophoresis
Quiz on DNA structure and function
Go over ABE Lab 2 Restriction Digest of pKan and pAra
Lab Activity: ABE Lab 3 Ligation
Due today - Strawberry DNA Isolation Lab report
Homework:
Do Biotech Online Chop and Go pg 124
Finish ABE Lab 1 Gel Electrophoresis – due on Thurs 10/31
Read ABE Lab 4 and 5 Confirmation of Digest and Transformation of HB101 with pAra-R
Do ABE Lab 4,5 Prelab Notes
Do Ch 4 Biotech Live 4.4-4.6
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7
F No School
Tuesday, Oct 29
In class:
Go over ABE Lab 3 Ligation of pAra and pKan
Lab Activity today: Lab 4 Confirmation of pAra and pKan by Gel Electrophoresis
Due today: CTE Modules 2 Framework Module 2
Homework:
Read ABE Lab 5 Transformation and review Lab 4 Confirmation of pAra and pKan
Finish Biotech Live 4.1-4.6
Do CTE DEI Module 3 - due Thursday, 11/7
ABE Lab 1 Gel Electrophoresis – due Thursday, 11/7
Thursday, Oct 31
In class:
Go over ABE Lab 5 Transformation Ligation and Lab 4 Confirmation of pAra and pKan
Lab 4: ABE Lab 5 Transformation with pARA-R
Due today: ABE Lab 1 Gel Electrophoresis
Homework:
Finish CTE DEI Module 3 – due Thursday, 11/7
Due: ABE Lab 4 Confirmation of Restriction Digest by Gel Electrophoresis
Finish ABE Labs 2 and 3 report – due Thursday, 11/7
Finish Ch 4 Packet – Review Questions and Ch 4.1-4.6, Biotech Online 4.1-4.6, Biotech Live 4.1-4.6 – due on Thursday, 11/7
Study for Ch 4 Test – Test on Thursday, 11/7
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Nov 5
In class:
Review ABE Labs 2-4 –Restriction Digest of pAra and pKan-R and Ligation
Lab Activity: ABE Lab 5 Transformation of E.coli with pAra-R
ABE Labs 2 and 3 lab reports due on Thursday, 11/7
Quiz on ABE Labs 1 -3
Homework:
Read ABE Lab 5 Transformation of E.coli with pAra-R
Complete ABE Labs 2 and 3 lab reports due on Thursday, 11/7
ABE Lab 4 Restriction Digest Confirmation – Due Tue 11/7
Study for Ch 4 Test – Test on Thurs 11/7
Finish Ch 4 Packet – includes Review Questions, Biotech Online, and Biotech Live; due Thurs 11/7
Thursday, Nov 7
In class:
Finish ABE Lab 5 – screen for red colonies and go over Bacterial Transformation
Hands-on Activity – HASPI Diagnosing Genetic Disease Through Gel Electrophoresis
Bioinformatics NCBI and Blast –we will do on Tue 11/12
Due today: ABE Labs 2 and 3 lab reports and Ch 4 packet
Ch 4 Test today
Homework:
Finish Activity on Diagnosing Genetic Disease Through Gel Electrophoresis – Due on Tue 11/12
Read ABE Lab 6 Overnight Culture of Transformed E.coli
Finish ABE Labs 4 Confirmation of Digest lab report – Due Tue 11/14
Monday – Veteran's Day, No School
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Nov 12
In class:
Review ABE Lab 5 Transformation and Lab 6 Overnight Culture
Hands-on Activity – HASPI Diagnosing Genetic Disease Through Gel Electrophoresis
Bioinformatics NCBI and Blast
Lab Activity today – Lab 6 Set up Overnight Culture of Transformed Cells
Homework:
Read Ch 5.1 Protein structure and function and do review questions
B.O. Antibody Producing Companies
ABE Lab 5, Transformation due Tues, 11/19
Read ABE Lab 7 Isolation of RFP by Column Chromatography; do prelab questions
ABE Lab 4 Confirmation of Digest lab report – due on Thurs, 11/14
Thursday, Nov 14
In class:
Go over ABE Lab 7 Isolation of RFP by Column Chromatography and Bioinformatics
Activity: Paper Plasmid – constructing recombinant DNA (if time permits)
Due today: ABE Lab 4 Confirmation of Digest lab report
Homework:
Lab Acivity: ABE Lab 7 Isolation of RFP by Column Chromatography lab report – due Thurs, 11/21
Read Ch 5.2 Protein Production and Protein Activity
B.O. Couch Potatoes pg 146
ABE Lab 5, Transformation due Tues, 11/19
M – Banking Day, P1-P7
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Nov 19
In class:
Ch 5.1 and 5.2 Protein Structure and Function
Go over HASPI Diagnosing Genetic Disorders by Electrophoresis
Go over ABE Lab 6 and 7 Isolating RFP using Column Chromatography
Activity – Paper recombinant plasmid construction
Due today: ABE Lab 5 Transformation
Homework:
Prepare Oral Presentations on Genetic Disorders from NCBI/BLAST Activity – presentations on Tues, 12/3
Read Ch 5.3 Protein Catalysts and do review questions
B.O. Catalysts for Better Health pg 151
ABE Lab 6/7 Isolation of RFP by Column Chromatography – due on Thurs 11/21
Thursday, Nov 21
In class:
Ch 5.2 and 5.3 Protein Production and Protein Activity; correct review questions
Quiz Ch 5.1 and 5.2 Protein Structure
Presentations on Genetic Disorders from NCBI/BLAST activity today
Due today: ABE Lab 6/7 Isolation of RFP by Column Chromatography
Homework:
Read Ch 5.4 Studying Proteins and Ch 5.5 Analyzing Proteins and do review questions
B.O. Protein Sequencers – pg 156
Genetic Disorders from NCBI/BLAST activity – Presentations will be on Tues, 12/3
HAPPY THANKSGIVING!
Thanksgiving Week – No School
Reminder: Read Ch 5.4 Studying Proteins and Ch 5.5 Analyzing Proteins and do review questions
B.O. Protein Sequencers – pg 156
Genetic Disorders from NCBI/BLAST activity – Presentations will be on Tues, 12/3
Happy Thanksgiving!
M – Banking Day, P1-P7
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Dec 3
In class:
Review Ch 5.1-5.3 Protein Enzymes and correct assignments
Due today -– Genetic Disorders from NCBI/BLAST activity – Presentations begin today
Lab Activity – Antibody-Antigen Test
Homework:
Read/review Ch 5.1-5.3 more on Proteins
Complete Antibody-Antigen Test lab report – due Tues, 12/10
Thursday, Dec 5
In class:
Review Enzymes and Studying Proteins
Continue Genetic Disorders Presentations
Lab Activity – Catalase Lab, parts A-D
Homework:
Graph results of Catalase Lab – lab report on Thurs 12/12
Read PAGE Lab (Polyacrylamide Gel Electrophoresis)
Review Ch 5.4 and Study for Ch 5 Test
Ch 5 Test – Tues, 12/10
M – Banking Day, P1-P7
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Dec 10
In class:
Correct Ch 5.3-5.4 Protein Analysis
Go over Enzymes and studying proteins
Catalase Lab – Lab report due Mon 12/16
Homework:
Read Ch 5.5 Applications of Protein Analysis and PAGE
Read Ch 5 Summary and Lab Practices pgs 158-159
Do Biotech Live Activity 5.1-5.3
Final exam: Chapters 1-5 on Mon, 12/16
Thursday, Dec 12
In class:
Go over Graphing Data from Catalase; Continue Catalase Lab
Review Ch 5 Proteins, Enzymes
Ch 5 Test
Homework:
Finish Catalase Lab – due Mon, 12/16
Study for Finals, Ch 1-5 –on Mon, 12/16
Monday P1-P6 Review Final Exam in P7
Tuesday P1, P2 Final Exam
Wednesday P3, P4 Final exam
Thursday P5, P6 Final exam
P7 Finals in Biotech – 1:30-3:30 pm Ch 5 Test and Final Exam Ch 1-5
Week 18 Dec 16-20, 2024 Biotech Finals Sem 1
Monday P1-P6 Review Final Exam in P7
Tuesday P1, P2 Final Exam
Wednesday P3, P4 Final exam
Thursday P5, P6 Final exam
P7 Finals in Biotech – 1:30-3:30 pm Ch 5 Test and Final Exam Ch 1-5
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, Jan 9
In class:
Review Ch 5 Proteins and PAGE, Polyacrylamide Gel Electrophoresis
Go over Synthetic Biology Project –due in 10 weeks
Assign chapters for group presentations
HW:
Read up on PAGE – protein analysis (Dr. Coba will lecture on how PAGE is used in their lab to analyze proteins
Read Ch 6.1 Assays and Do review questions 6.1; write questions on separate sheet and answer in complete sentences
Brainstorm ideas for Synthetic Biology Project
Click here: https://biobuilder.org/lab/biology-by-design/?a=student
Day 2 Thursday, Jan 11
In class:
Set up PAGE experiment – Fish protein analysis with special guest, Dr. Coba and Mrs. Coba
Go over Synthetic Biology – go to www.Biobuilder.org
Ch 6.1 Potential Products
Lab Activity: PAGE
HW:
Read Ch 6.2 Assays and Do review questions 6.2
PAGE Fish Proteins – Lab report due on Thurs, Jan 18
Brainstorm ideas for Synthetic Biology Project
Click here: https://biobuilder.org/lab/biology-by-design/?a=student
Chapter Assignments: Please prepare a Powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp 6 – Ch 7.2, 7.3
Grp 7 – Ch 8.1-8.5
Grp 8 – Ch 9.1-9.5
Grp 1,2,3 – Ch 12
Grp 6,7,8 – Ch 13
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 1/16
In class:
Review PAGE – staining and destaining gel
Review Ch 6.1 Potential Biotech Product and Ch 6.2 The use of Assays, correct review questions
Group 1 Presentation - Ch 6.3 ELISA, correct review questions
Lab Activity – Finish destaining PAGE gel; Rapid ELISA, TRU RSV - Due on Tues 1/23
HW:
Read Ch 6.3 ELISA and do review questions 6.3
Do Biotech Online ELISA Technology in Diagnostic Kits pg 173
PAGE Lab report – due on Tues, 1/23
Due on Thurs, 1/23 – Rapid ELISA, TRU RSV
Day 2 Thursday, 1/18
In class:
Review Ch 6.2 Assays
Group 1 Presentation - Ch 6.2 The use of Assays, and Ch 6.3 ELISA, correct review questions
Lab Activity: Lab 6b and c Assaying for Starch, Sugar and Amylase Activity – due Tue 1/30
HW:
Read ELISA Lab
Finish Lab 6c Amylase Activity – due Thurs 1/30
Edpuzzle – ELISA
Continue working on Synthetic Biology Project
Chapter Assignments: Please prepare a power point presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp 6 – Ch 7.2, 7.3
Grp 7 – Ch 8.1-8.5
Grp 8 – Ch 9.1-9.5
Grp 1,2,3 – Ch 12
Grp 6,7,8 – Ch 13
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 1/23
In class:
Review Ch 6.3 ELISA and Amylase
Lab Activity: Lab 6b Assay for Starch and Sugar and Lab 6c Amylase Lab
HW:
Read ELISA Lab – Lab on Thursday
Read Ch 6.4 Western Blots and do review questions
Lab 6b Assay for Starch and Lab 6c Assaying for Amylase Activity – due Tues 1/30
Day 2 Thursday, 1/25
In class:
Review Lab 6c Amylase
Group Presentation – Ch 6.4 Western Blot
Lab Activity: Anti-Heparin ELISA Assay
HW:
Read Ch 6.5 Looking for new products
Do B.O. pg 178 Amazon Hide and Seek
Lab 6b Assay for Starch and Lab 6c Assaying for Amylase Activity – due Tues 1/30
Continue working on Synthetic Biology Project
Bring Herbs for Tuesday and read Lab 6d Looking for Potential Medicine
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp 1 - Ch 6.1-6.3
Grp 2 – Ch 6.4-6.6
Grp 3 – Ch 7.1, 7.4, 7.5
Grp 6 – Ch 7.2, 7.3
Grp 7 – Ch 8.1-8.5
Grp 8 – Ch 9.1-9.5
Grp 1,2,3 – Ch 12
Grp 6,7,8 – Ch 13
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 1/30 Stem Cell PCC Module: Feb 6-Feb 20
In class:
Review Ch 6.3 and 6.4 Western Blot and go over ELISA Lab
Group presentation – Ch 6.4 and 6.5 Looking for new products
Due today: Lab 6g HRP Isolation, Lab 6h Confirm HRP with TMB
Lab Activity – Lab 6h Confirm HRP with TMB
Lab 6d Looking for new products, continue on Thurs
HW:
Read Ch 6.6 Producing rDNA and do review questions
Read ELISA Anti-Heparin Lab – lab on Thursday 2/1
Lab 6g HRP Isolation – due on Tues 2/6
Day 2 Thursday 2/1
In class:
Lab Activity: ELISA Anti-Heparin Lab
Group Presentation – Ch 6.6 Producing rDNA and correct review questions
Lab Activity: ELISA Anti-Heparin Lab
HW:
Do Biotech Live Activity 6.1-6.4 – due on Thurs 2/8
Read Lab 6d Looking for new products – Do on Tues
Ch 6 Test – Thurs 2/8; Ch 6 review questions - due on Thurs 2/8
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 2/6 (STEM CELL MODULE POSTPONED UNTIL MARCH)
In class:
Go over Ch 6.4 Western Blot
Group Presentation – Ch 6.5 Looking for New Products, do review questions
Lab Activity: Lab 6d Looking for new products – bring herbs
HW:
Read Ch 6.6 Producing rDNA and do review questions
Do Biotech Online pg 163 Quality Not and Quantity
Finish Lab 6g and 6h Isolation of HRP and testing against TMB (on same lab report)- due on Tues 2/8
Day 2 Thursday 2/8
In class:
Group Presentation – Ch 6.6 Producing rDNA and correct review questions
Due today: Lab 6g and 6h Isolation of HRP and testing against TMB (on same lab report)-
Lab Activity: Continue Lab 6d Looking for new products, test against bacteria
HW:
Complete
Finish Biotech Live Activity 6.1-6.4 – due on Tues 2/13
Ch 6 Test – Tues 2/13; Ch 6 review questions- due on Tues 2/13
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 2/13
In class:
Go over Ch 6 and correct all assignments
Ch 6 Assay Test; Ch 6 packet – due today
Lab Activity: Lab 6d Looking for new products - make final observations, lab report due on Tues 2/20
HW:
Read Ch 7.1 Spectrophotometer and do review questions pgs 195-197
Do B.O. 7.1 FTIR, pg 200
Edpuzzle: Spectrophotometer – take notes; turn in on Tue 2/20
Read Lab 7a and 7b How to Use a Spectrophotometer
Day 2 Thursday, 2/15
In class:
Go over Lab 6d Antimicrobial properties
Group presentation on Ch 7. 1 Spectrophotometer
Lab Activity - Lab 7a and 7b How to Use a Spectrophotometer
HW:
Read Ch 7.4 and 7.5 Use of Spectrophotometers to measure Protein Concentration
Do 7.4 and 7.5 Review questions
Finish Lab 7a and 7b lab reports – due on Thurs 2/22
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 2/20
In class:
Review Ch 7.1 Spectrophotometers
Review Labs 7a and 7b How to use a spectrophotometer
Lab Activity: Soda Lab using the Spectrophotometer; create excel file, convert %T to A
Due today: Lab 6d Looking for Potential Medicine lab reports
Quiz on Ch 7.1 Spectrophotometer
HW:
Read Ch 7.4 and 7.5 Use of Spectrophotometers to measure Protein Concentration
Do 7.4 and 7.5 Review questions
Finish Lab 7a and 7b lab reports – due on Thurs, 2/22
Soda Lab using the Spectrophotometer lab report – due Tues, 2/27
Day 2 Thursday, 2/22
In class:
Review Spectrophotometers, Soda Lab using the Spectrophotometer and conversion A to %T
Group Presentation today: Ch 7.4 and 7.5 Using Spectrophotometers to measure Concentration
Due today: Lab 7a and 7b lab reports
HW:
Read Ch 7.2 and do review questions
Do B.O pg 203
Soda Lab using the Spectrophotometer lab report – due Tues, 2/27
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 2/27
In class:
Grp presentation 7.5 Using Spectrophotometers to measure Concentration and
Ch 7.2 pH and correct review questions pgs 200-203
Learn how a pH meter electrode works
Lab Activity – pH Lab – due on Tues, 3/5
Due today -- Soda Lab using the Spectrophotometer lab report
HW:
Finish Ch 7.2 review questions and B.O. 200 and 203
Finish pH Lab – due on Tues, 3/5
Do pH problems – due on Thurs, 2/29
Day 2 Thursday, 2/29
In class:
Review 7.2 review questions and B.O. 200 and 203
Go over pH problems
Group presentation on Ch 7. 3 Buffer
Video: Buffer Balancing Acts, https://www.youtube.com/watch?v=7ciO9eO3OQg
HW:
Read Ch 7.3 Buffers and do review questions
Do B.O. pg 208 How and why proteins are purified from cells
Do Biotech Live Activity 7.1 Spectrophotometer and 7.2 Phun at Home with pH
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Fri Minimum day
Day 1 Tuesday, 3/5
In class:
Review Ch 7.3 Buffer
Read Ch 7 Summary and Lab Practices
EP: Buffer Balancing Acts, take notes and take quiz
Due today: pH Lab
HW:
Study Ch 7 Summary and Lab Practices – Ch 7 Test on Thurs 3/7
Do Thinking Like a Biotechnician #1-10 pg 216
Finish Biotech Live Activity 7.1-7.2 pg 216-217
Ch 7 packet – due on Thurs 3/7
Soda Lab – due on Thurs 3/7
Electrophoresis, Buffer, and pH Lab – due on Tues 3/12
Day 2 Thursday, 3/7
In class:
Grp presentation – Ch 7.3 Buffers
Ch 7 Spectrophotometers Test
Ch 7 Packet due today
Due today - Soda Lab
HW:
Read Ch 8.1 The Production of Recombinant Biotech Product; do review questions
Do B.O. Some Say Genetic Engineering.. pg 226
Electrophoresis, Buffer, and pH Lab – due on Tues 3/12
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7
Fri P1-P7 Minimum day
Day 1 Tuesday, 3/12
In class:
Group Presentation: Ch 8.1 The Production of Recombinant Biotech Product; correct review questions
Lab Activity: Lab 1 Gel Electrophoresis to practice pipetting micro-volumes; calculate and pour gel
Work on Synthetic Biology Project – learn how to use Tinker Cell
HW:
Read Ch 8.2 Transforming Cells pgs 227-234 and do review questions
Do B.O. BACs and YACs pg 239; Endonucleases pg 230; RFLPs pg 231
Read ABE Lab 2 Restriction Digest pGrn and pDrk with HindIII and Lab 3 Ligation
Continue working on Synthetic Biology Project
Day 2 Thursday, 3/14
In class:
Grp presentation – Ch 8.2 Transforming Cells and correct review questions
Lab Activity: ABE Lab 2 Restriction Digest - Digest pGrn and pDrk with HindIII
Work on Synthetic Biology and Tinker Cell
HW:
Read ABE Lab 3 Ligation to create a recombinant rpGlo
Complete ABE Lab 2 Restriction Digest pGrn and pDrk report – due on Tues 3/26
Work on Synthetic Biology project – due on Tues April 2
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
SPRING BREAK
HW from Tuesday, 3/12
Read Ch 8.2 Transforming Cells pgs 227-234 and do review questions
Do B.O. BACs and YACs pg 239; Endonucleases pg 230; RFLPs pg 231
Read ABE Lab 2 Restriction Digest pGrn and pDrk with HindIII and Lab 3 Ligation
Continue working on Synthetic Biology Project
HW from Thursday, 3/14
Read ABE Lab 3 Ligation to create a recombinant rpGlo
Complete ABE Lab 2 Restriction Digest pGrn and pDrk report – due on Tues 3/26
Work on Synthetic Biology project – due on Tues April 2
If you have not turned in your lab reports, please post them soon! Thanks!
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Ch 8.2 and 8.3 Don't forget to make a ppt presentation ready to present on Tuesday
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
Have a restful Spring Break!
M Professional Development; No students on campus
T/Th P1, P3, P5, P7 (Thursday – Health/Wellness Assembly)
W/Fri P2, P4, P6, P7
Day 1 Tuesday, 3/26
In class:
Review ABE (Green) Lab 2 Restriction Digest of pDrk and pGrn
Lab Activity: ABE (Green) Lab 3 Ligation of cut pDrk and pGrn – lab report due Tues, 4/2
Group Presentation – Ch 8.2 Transformation and review questions
Work on Synthetic Biology
Due today: ABE Lab 2 Restriction Digest of pDrk and pGrnf
HW:
Review Ch 8.2 Transforming Cells
Finish B.O. BACs and YACs pg 239; Endonucleases pg 230; RFLPs pg 231
Read Ch 8.3 After Transformation and do review questions
Do B.O pg 238 You can Try This at Home
Read ABE (Green) Lab 5 Transformation
Day 2 Thursday 3/28
In class:
Review ABE (Green) Lab 3 Ligation to construct rpGLO
Review Transformation, Ch 8.2 and Lab 5 Transformation
Lab Activity: ABE (Green) Lab 5 Transformation of HB101 with rpGLO - lab report due Tues, 4/2
HW:
ABE Labs 3 Ligation to construct rpGLO – lab report due Thurs, 4/4
Read Ch 8.4 Fermentation, Manufacturing, and GMP and do review questions
Read ABE(Green) Lab 4 Confirmation of Restriction Digest of pGRN and pDRK
Synthetic Biology Project, Group presentations begin on Tues, 4/9
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp 1,2,3,6,7,8 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 4/2
In class:
Review ABE (Green) Lab 5 Transformation of HB101 with rpGLO - lab report due today, Tues, 4/2
Grp Presentation - Ch 8.4 Fermentation, Manufacturing, and GMP and do review questions
and Ch 8.3 After Transformation and do review questions
Lab Activity: Lab 4 Confirmation of Digest of pDrk and pGrn and do prelab notes,
Continue Lab 5 Transformation – look for green glowing cells
HW:
ABE Labs 3 Ligation to construct rpGLO – lab report due Thurs, 4/4
ABE (Green) Lab 5 Transformation of HB101 with rpGLO - lab report due Tues, 4/9
ABE Lab Activity: Lab 4 Confirmation of Digest of pGRN and pDrk, due Thurs, 4/11
Read Ch 8.5 Retrieving Plasmids after Transformation
Day 2 Thursday, 4/4
In class:
Review ABE Lab 4 Confirmation of Digest of pDrk and pGrn
Grp Presentation - Ch 8.5 Retrieving Plasmids after Transformation correct review questions
Do Biotech Live 8.1-8.4 pgs 248-250
Due today: ABE Labs 3 Ligation to construct rpGLO lab report
HW:
Finish Biotech Live 8.1-8.4 pgs 248-250 and all assignments
ABE (Green) Lab 5 Transformation of HB101 with rpGLO - lab report due Tues, 4/9
ABE Lab Activity: Lab 4 Confirmation of Digest of pGRN and pDrk, due Thurs, 4/11
Ch 8 Test on Tuesday, 4/9; Ch 8 packet due 4/9
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 4/9
In class:
Review Ch 8 and ABE Labs 1-5
Ch 8 test today
Lab Activity: ABE Lab 4 Confirmation of restriction digest
Due today: Ch 8 packet includes review questions, B.O. 8.1-8.5, and Biotech Live 8.1-8.5
Due today: ABE (Green) Lab 5 Transformation of HB101 with rpGLO - lab report
Set up culture for Mini-prep
HW:
Read Ch 9. 1 Bringing Product to Market, do review questions
Read Mini-prep lab - Lab on Thursday
Day 2 Thursday 4/11
In class:
Group presentation: Ch 9.1 Bringing Product to Market
Lab Activity: Mini-prep lab
HW:
Finish Mini Prep lab – lab report due on Thurs 4/18
Read Ch 9.2 Using Chromatography to study molecules and do review questions
Get ready for DNA Barcoding – read isolating DNA from bees
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
M P1-P7
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 4/16
In class:
Go over ABE Lab 4 Confirmation of Restriction Digest
Review Ch 9.1 Bringing Product to Market
Group Presentation – Ch 9.2 Using Chromatography to study molecules
Lab Activity: Mini-prep
HW:
ABE Lab 4 Confirmation of Restriction Digest Lab Report – due on Thurs 4/18
Read Ch 9.3 Column Chromatography: An expanded discussion; do review questions
Do B.O. Got Gas pg 268
Mini-prep lab report – due Tues, 4/23
Day 2 Thursday, 4/18
In class:
Review Ch 9.2 Chromatography and mini-prep
Group Presentation – Ch 9.3 Column Chromatography
Lab Activity: Column Chromatography
HW:
Finish Mini-prep lab report – due Tues, 4/23
Column Chromatography lab report – due Thurs, 4/25
Read Ch 9.4 Product Quality Control and do review questions
Do B.O. Approved Biotechnology Drugs pg 273
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
M/Th P1, P3, P5, P7
Wednesday – No School
W/F P2, P4, P6, P7
Day 1 Tuesday, 4/23
In class:
Intro to DNA Barcoding
DB Lab 1 and Lab 2 – Isolate DNA from Bees
Due today: ABE Lab 4 Confirmation of Digest
HW:
Read Intro to DNA Barcoding: DB Lab 1 and Lab 2 – Isolate and purify DNA from Bees
Do Ch 9 Biotech Live Activity 9.1-9.3
Ch 9 Test on Tues 4/30
Day 2 Thursday 4/25
In class:
Go over Purifying DNA from Bees
Lab Activity: DB Lab 2 Purify DNA from Bees
HW:
Read DB Lab 3 Casting Gel and DB Lab 4 Gel Electrophoresis
Read Ch 9 Summary and Lab Practices
Ch 9 Test on Tues 4/30
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 4/30
In class:
Review DB Labs 1 and 2 Purifying gDNA
Lab Activity DB Lab 1 and Lab 2 – Purifying total DNA (gDNA) from tissue lysates
Ch 9 Test today
HW:
DB Labs 1 and 2 Isolate and Purify DNA from Bees – due Thursday 5/9
Read DB Lab 3 and 4 Examining gDNA using Gel Electrophoresis
Day 2 Thursday 5/2
In class:
Go over Purifying DNA from Bees using spin column (chromatography)
Go over Chromatography
Lab Activity: DB Lab 3 and 4 Examining gDNA using Gel Electrophoresis
HW:
Read DB DB Lab 5 Targeted amplification of the COI barcode region from a gDNA template
DB Labs 1 and 2 Isolate and Purify DNA from Bees lab report – due Thursday 5/9
DB Lab 3 and 4 Examining gDNA using Gel Electrophoresis lab report – due on Tue, 5/14
Read DB Lab 5 Targeted amplification of the COI barcode region from a gDNA
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
Monday – P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 5/7
In class:
Go over DB Labs 3 and 4 Confirming gDNA by gel electrophoresis
Lab Activity: DB Labs 3 and 4 Confirming gDNA by gel electrophoresis
HW:
DB Labs 1 and 2 Isolate and Purify DNA from Bees lab report – due Thurs 5/9
DB Labs 3 and 4 Confirming gDNA by gel electrophoresis – due Thurs 5/16
Read DB Lab 5 Amplification of CO1 by PCR
Day 2 Thursday 5/9
In class:
Go over DB Lab 5 Targeted amplification of the COI by PCR
Lab Activity: DB Lab 5 Amplification of CO1 by PCR
Due today: DB Labs 1 and 2 Isolate and Purify DNA from Bees lab report
HW:
Read DB Lab 6: Spin Column to purify CO1 amplicons
DB Labs 3 and 4 Confirming gDNA by gel electrophoresis – due Thurs 5/16
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp8 – Ch 12
Grp9 – Ch 13
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 5/14
In class:
Lab Activity today: DB Lab 5 CO1 Amplification
Go over DB Lab 3 and 4 Spin Column to purify gDNA and confirm by gel electrophoresis
HW:
DB Labs 3 and 4 Confirming gDNA by gel electrophoresis lab report – due Thurs 5/16
Read DB Lab 6 Spin Column to purify CO1 gene
DB Lab 5 CO1 Amplification lab report – due Thurs 5/23
Synthetic Biology Project due on Thurs 5/23
Day 2 Thursday 5/16
In class:
Go over DB Lab 5 CO1 Amplification
Due today: DB Labs 3 and 4 Confirming gDNA by gel electrophoresis lab report
Lab Activity: DB Lab 6 Spin Column to purify CO1 gene
HW:
Read DB Lab 7 and 8 Gel Electrophoresis to Confirm CO1
DB Lab 5 CO1 Amplification lab report – due Thurs 5/23
Read Ch 12.1 Medical Biotechnology Synthesis and do review questions
Do Ch 12.1 B.O. New Medications and Penicillin pgs 344-345
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp 1,2,3 – Ch 12
Grp 6,7,8 – Ch 13
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 5/21
In class:
Go over DB Lab 6 pin Column to purify CO1 gene – due Thurs 5/23
Lab Activity today: B Lab 7/8 Confirmation of CO1 by Gel Electrophoresis
Group Presentation – Ch 12.1 Drug Discoveries
HW:
Read Ch 12.1-12.2 Medical Biotechnology and Pharmaceuticals Synthesis and do review questions
Do Ch 12.1 B.O. New Medications and Penicillin pgs 344-345
DB Lab 7/8 Confirmation of CO1 by Gel Electrophoresis – due on Tues, 5/28
Day 2 Thursday 5/23
In class:
Go over DB Lab 7/8 Confirmation of CO1 by Gel Electrophoresis
Group Presentation - Ch 12.2 Creating Pharmaceuticals and 12.3 Creating Pharmaceuticals
Due today: DB Lab 6 pin Column to purify CO1 gene – due Thurs 5/23
HW:
Read 12.3 Creating Pharmaceuticals; do review questions
Read Ch 12.4 Creating Pharmaceuticals by Peptide/Antibody Engineering and do review questions
Do B.O. Getting Sick is No Laughter pg 354 and B.O. Eating Vegetables pg 355
DB Lab 7/8 Confirmation of CO1 by Gel Electrophoresis – due on Tues, 5/28
Study for LAMC finals – Finals: Friday, 5/29
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp 1,2,3 – Ch 12
Grp 6,7,8 – Ch 13
Monday – Memorial Day Holiday – No School
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, May 28
In class:
Group Presentation on Ch 12.3 Creating Pharmaceuticals
Presentation on Synthetic Biology Project
HW:
Finish your Career Exploration Project – presentations on Thursday, May 30
Finish your resume
Study for Finals Ch 1-9, 12 - Finals on Monday, June 3
Day 2 Thursday May 30
In class:
Career Exploration Project – presentations on today
Due today: Resume
HW:
Study for Finals – Finals on Monday, June 3
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Oct 17
In class:
Go over 4.3 DNA Structure and Isolation of DNA
Quiz on Ch 4.1-4.3
Go over ABE Lab 1 Gel Electrophoresis
Lab Activity: ABE Lab 2 Restriction Digest of pKan and pAra
Due today: Strawberry DNA Isolation Lab
Homework:
Read Ch 4.4 Gel Electrophoresis and do review Questions
Read ABE Lab 3 Ligation
Finish ABE Lab 2,3 Prelab Notes
Do B.O. Chop and Go Electrophoresis pg 124
Do Ch 4 Biotech Live 4.1-4.2
ABE Lab 1 Gel Electrophoresis – due on Tuesday 10/24
Thursday, Oct 19
In class:
Go over Ch 4.4 Gel Electrophoresis
Quiz on Gel Electrophoresis
Go over ABE Lab 2 Restriction Digest of pKan and pAra
Lab Activity: ABE Lab 3 Ligation
Homework:
Do Biotech Online Chop and Go pg 124
Finish ABE Lab 1 Gel Electrophoresis – due on Tuesday 10/24
Read ABE Lab 4 and 5 Confirmation of Digest and Transformation of HB101 with pAra-R
Do ABE Lab 4,5 Prelab Notes
Read Ch 4 Summary and Lab Practices pgs 126-127
Do Ch 4 Biotech Live 4.4-4.6
Ch 4 Test on Thursday, 10/26
M/Th P1, P3, P5, P7
W PSAT 8:30-12:30
T/F P2, P4, P6, P7
Tuesday, Oct 9
In class:
Go over Math Problems
Ch 4.1 DNA Structure
Lab Activity: Isolation of Strawberry DNA
Due today: Student-written procedure on micropipetting with red dye; Math Problems
Homework:
Read Ch 4.2 Sources of DNA, do review questions
Do Biotech Online: Know your Genome pg 112, Baldness Gene pg 118
Download Amgen Lab Manual – Read ABE Lab 1 Gel Electrophoresis and do prelab notes
Strawberry DNA Lab – due on Tues, 10/17
Thursday, Oct 11
In class:
Ch 4.2 Sources of DNA, correct review questions
Activity: Cast a Gel for Gel Electrophoresis; make 1x SB buffer and 1% agarose gel;
- ABE Lab 1 – Gel Electrophoresis
Homework:
Read Ch 4.3 Isolating and Manipulating DNA
Do Biotech Online Recombinant Pharmaceuticals and Therapies, pgs 118-119
Read Lab 2 Amgen Lab Manual – pKan and pARA Digest with restriction enzymes
Strawberry DNA Lab – due on Tues, 10/17
08/28/202308/28/2023
M - P1-P7 (Banking Day)
T/Th - P1, P3, P5, P7
W/F - P2, P4, P6, P7
Tuesday, August 29
In Class:
What is Scientific Method?
Correct Ch 1.2-1.3 review questions
Lab Activity: Cheese Lab, make first observation, record in your notebook
Biotech Live: Activity 1 What is Biotechnology? pgs 32-33
Activity 2 Business Side pgs 33-34
Bleach Lab due today– Did you send to intern to check for content/format?
HW:
Read ED Ch. 1.4 and review questions pgs 19-23 ED
Do B.O. Hot Jobs pg 26
Extra Credit: make cheese at home; DIY on foodwishes.com
Thursday, August 31
In Class:
Cheese Lab - Make 2nd observation, record in your notebook ready for cheese cloth?
Discuss Careers in Biotech
Do Biotech Live Activity 1.4 How Biotech Industry Improving Human Life? pg 35
Correct Ch 1.4 review questions
HW:
Read ED Ch 1.5-1.6 and do review questions pgs 23-29 ED
Do B.O. Code of Business Conduct pg 29 ED
Read Summary and Lab Practices pgs 30-31
Please share your lab report with your intern to get it checked before turning it in to me.
Biotech Interns: Here are your interns:
Caris (ckim7961@stu.gusd.net):Last names A-Hovanes
Ani (ahar1308@stu.gusd.net): Last names I-Markarian
Irene (ikim5843@stu.gusd.net) and Emily (egai0346@stu.gusd.net): Last names Montecello-V
You can also get help from Stella (ster9435@stu.gusd.net) and Emi (enak1491@stu.gusd.net)
08/21/202308/21/2023
M - P1-P7 (Banking Day)
T/Th - P1, P3, P5, P7
W/F - P2, P4, P6, P7
Day 1 Tuesday, Aug 22
In class:
What is Biotech?
Go over How to maintain a Scientific Notebook in Biotech
Lab Activity: Bleach Lab pgs 19-21 TB
HW:
Read Ch 1.1-1.2 and do review questions Ch 1.1-1.2 in ED - write questions and answers on paper with black ink
Read Cheese Lab before Thursday and bring whole milk and buttermilk on Thursday
Do Biotech Online, B.O., pg 13 Glo Fish
Day 2 Thursday, Aug 24
In class:
Complete and make final observations for Bleach Lab, lab report due on Th 8/31
Review How to Notebook and how to write an effective conclusion
Lab Activity: Cheese Lab
HW:
Read Ch 1.3 pgs 14-18 and do review questions 1.3 ED
Do Biotech Online (B.O.) pg 18 Genentech
Finish Bleach Lab report in Google Docs pgs 19-21 ED; lab report due on Th 8/31
08/15/202308/15/2023
Day 1 Wednesday, August 16
In class:
Rules and Policies
Safety Rules
HW:
Sign up for Google Classroom, Quizizz, Edpuzzle
Study for Safety Test – Test on Thursday
Wear shoes to class – Always!
Day 2 Thursday, August 17
In class:
Safety Test
What is Biotechnology
HW:
Read Ch 1.1 Biotechnology ED (Ellyn Daugherty textbook)
Read Bleach Lab pgs 19-21 ED, Lab on Tuesday
Wear shoes, = please!
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7
F No School
Tuesday, Oct 24
In class:
Go over ABE Lab 3 Ligation of pAra and pKan
Lab Activity today: ABE Lab 5 Transformation
Do/Finish CTE Modules 2 & 3
Due today: ABE Lab 1 Gel Electrophoresis
Homework:
Read ABE Lab 5 Transformation and Lab 4 Confirmation of pAra and pKan
Finish Biotech Live 4.1-4.6
Do/Finish CTE Modules 2 & 3
Study for Ch 4 Test – Test on Thursday, 10/26
Thursday, Oct 26
In class:
Ch 4 Test
Go over ABE Lab 5 Transformation Ligation and Lab 4 Confirmation of pAra and pKan
Lab 4: Lab 4 Confirmation of pAra and pKan by Gel Electrophoresis
Homework:
Do/Finish CTE Modules 2 & 3
Due: ABE Lab 4 Confirmation of Restriction Digest by Gel Electrophoresis
Finish ABE Labs 2 and 3 report – due Thursday, 11/2
Finish Ch 4 Packet – Review Questions Ch 4.1-4.4, Biotech Online 4.1-4.4, Biotech Live 4.1-4.6 – due on Tuesday, 11/2
M/W P1, P3, P5
T/Th P2, P4, P6, P7
F P1-P7 Shakeout Assembly Schedule
Tuesday, Oct 31
In class:
Review ABE Labs 2-4 –Restriction Digest of pAra and pKan-R and Ligation
Lab Activity: ABE Lab 5 Transformation of E.coli with pAra-R
ABE Labs 2 and 3 lab reports due on Thursday, 11/2
Due today: Ch 4 Packet
Quiz on ABE Labs 1 -3
Homework:
Read ABE Lab 5 Transformation of E.coli with pAra-R
Complete ABE Labs 2 and 3 lab reports due on Thursday, 11/2
ABE Lab 4 Confirmation – Due Tue 11/7
Finish Ch 4 Packet – due Thurs 11/2
Thursday, Nov 2
In class:
Finish ABE Lab 5 – screen for red colonies and go over Bacterial Transformation
Hands-on Activity – HASPI Diagnosing Genetic Disease Through Gel Electrophoresis
Bioinformatics NCBI and Blast – If time permits or we will do on Tue 11/7
Due today: ABE Labs 2 and 3 lab reports and Ch 4 packet
Quiz on Restriction digest and Gel Electrophoresis
Homework:
Finish Activity on Diagnosing Genetic Disease Through Gel Electrophoresis – Due on Tue 11/7
Read ABE Lab 6 Overnight Culture of Transformed E.coli
Finish ABE Labs 4 Confirmation of Digest lab report – Due Tue 11/7
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7
Friday – Veteran's Day, No School
Tuesday, Nov 7
In class:
Review ABE Lab 6 Set up Overnight Culture of Transformed Cells
Lab Activity today – ABE Lab 7 Isolation of RFP by Column Chromatography
Quiz on ABE Lab 4, 5 Confirmation, and Transformation
Due today: ABE Lab 4 Confirmation of Digest lab report
Homework:
Read Ch 5.1 Protein structure and function and do review questions
B.O. Antibody Producing Companies
ABE Lab 5, Transformation due Thursday, 11/9
Read ABE Lab 7 Isolation of RFP by Column Chromatography; do prelab questions
Thursday, Nov 9
In class:
Go over ABE Lab 7 Isolation of RFP by Column Chromatography;
Activity: Paper Plasmid – constructing recombinant DNA
Due today: ABE Lab 5, Transformation
Homework:
Finish ABE Lab 7 Isolation of RFP by Column Chromatography lab report – due Thursday, 11/16
Read Ch 5.2 Protein Production and Protein Activity
B.O. Couch Potatoes pg 146
M – Banking Day, P1-P7
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Nov 14
In class:
Ch 5.1 and 5.2 Protein Structure and Function
Go over HASPI Diagnosing Genetic Disorders by Electrophoresis – due today
Activity – Paper recombinant plasmid construction
Quiz Ch 5.1 Protein Structure
Homework:
Read Ch 5.3 Protein Catalysts and do review questions
B.O. Catalysts for Better Health pg 151
ABE Lab 6/7 Isolation of RFP by Column Chromatography – due on Thurs 11/16
Thursday, Nov 16
In class:
Ch 5.2 and 5.3 Protein Production and Protein Activity; correct review questions
Activity: Genetic Disorders from NCBI/BLAST activity – Presentations will be on Tues, 11/28
Due today: ABE Lab 6/7 Isolation of RFP by Column Chromatography
Homework:
Read Ch 5.4 Studying Proteins and Ch 5.5 Analyzing Proteins and do review questions
B.O. Protein Sequencers – pg 156
Genetic Disorders from NCBI/BLAST activity – Presentations will be on Tues, 11/28
HAPPY THANKSGIVING!
Thanksgiving Week – No School
Reminder: Read Ch 5.4 Studying Proteins and Ch 5.5 Analyzing Proteins and do review questions
B.O. Protein Sequencers – pg 156
Genetic Disorders from NCBI/BLAST activity – Presentations will be on Tues, 11/28
Happy Thanksgiving!
M – Banking Day, P1-P7
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Nov 28
In class:
Review Ch 5.1-5.3 Protein Enzymes and correct assignments
Due today -– Genetic Disorders from NCBI/BLAST activity – Presentations begin today
Lab Activity – Antibody-Antigen Test
Homework:
Read/review Ch 5.1-5.3 more on Proteins
Complete Antibody-Antigen Test lab report – due Tues, 12/5
Thursday, Nov 30
In class:
Review Enzymes and Studying Proteins
Continue Genetic Disorders Presentations
Lab Activity – Catalase Lab, parts A-D
Homework:
Graph results of Catalase Lab
Read PAGE Lab (Polyacrylamide Gel Electrophoresis)
Review Ch 5.4 and Study for Ch 5 Test
Ch 5 Test – Thurs, 12/7
M – Banking Day, P1-P7
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Dec 5
In class:
Due today -– Antibody-Antigen: HCG and iFOBT (Occult Blood) Lab reports
Correct Ch 5.3-5.4 Protein Analysis
Go over Enzymes and studying proteins
Catalase Lab – Lab report due Tues 12/12
Continue with Genetic Disorder Presentations
Homework:
Study for Ch 5 Test and Complete Ch 5 Packet – Test on Thurs 12/7
Read Ch 5.5 Applications of Protein Analysis and PAGE
Read Ch 5 Summary and Lab Practices pgs 158-159
Do Biotech Live Activity 5.1-5.3
Thursday, Dec 7
In class:
Go over Graphing Data from Catalase; Continue Catalase Lab
Review Ch 5 Proteins, Enzymes
Ch 5 Test
Homework:
Finish Catalase Lab – due Tues, 12/13
Read PAGE Polyacrylamide Gel Electrophoresis – do on Tues, Dec 12
Study for Finals, Ch 1-5 – Finals on Thurs 12/14
06/05/202306/05/2023
M Finals in P1, P2
Review in P3, P4
T Finals in P3, P4
Review in P5, P6
W Finals in P5, P6
Please bring back your textbook to room 2205. We need it for our summer class.
DO NOT BRING IT TO THE LIBRARY.
No meeting this week for Biotech
Have a Great Summer Vacation!
05/29/202305/29/2023
Monday – Memorial Day Holiday – No School
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Wednesday, 5/31
In class:
DNA Barcoding: Bioinformatics; Look at Trace Files and barcodes, enter metadata
Group Presentation on Ch 12.3 Creating Pharmaceuticals
HW:
Study for Finals Ch 1-9, 12 - Finals on Friday, 6/2
Day 2 Thursday 6/1
In class:
DNA Barcoding: Bioinformatics; Finish editing and uploading Barcodes
HW:
Read DB Lab 7 Confirmation of CO1 Amplicons
Study for Finals – Finals on Friday, 6/2
Day 3 Friday, 6/2
In class:
Finals
05/22/202305/22/2023
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 5/23
In class:
Group Presentation – Ch 12.1 Drug Discoveries
DNA Barcoding – go over PCR
HW:
Read Ch 12.2 Creating Pharmaceuticals and 12.3 Creating Pharmaceuticals; do review questions
Start studying for LAMC finals
Day 2 Thursday 5/25
In class:
Group Presentation - Ch 12.2 Creating Pharmaceuticals and 12.3 Creating Pharmaceuticals
HW:
Read Ch 12.4 Creating Pharmaceuticals by Peptide/Antibody Engineering and do review questions
Do B.O. Getting Sick is No Laughter pg 354 and B.O. Eating Vegetables pg 355
Study for LAMC finals
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp 1,2,3 – Ch 12
Grp 6,7,8 – Ch 13
05/15/202305/15/2023
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 5/16
In class:
Due today: DB Lab 5 CO1 Amplification
DB Lab 7 and 8 Gel Electrophoresis to Confirm CO1
HW:
Read Ch 9 Summary and Lab Practices
Study for Ch 9 Test – Test on Thursday
DB Lab 6 Spin Column to purify CO1 gene - due on Thurs
Day 2 Thursday 5/18
In class:
Due today: DB Lab 6 pin Column to purify CO1 gene
Ch 9 Test today
Go over Bioinformatics
HW:
Read Ch 12.1 Medical Biotechnology Synthesis and do review questions
Do Ch 12.1 B.O. New Medications and Penicillin pgs 344-345
DB Lab 7/8 Confirmation of CO1 by Gel Electrophoresis – due on Tues, 5/23
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp8 – Ch 12
Grp9 – Ch 13
05/08/202305/08/2023
Monday – P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 5/9
In class:
Review DB Labs 3 and 4 Confirming gDNA by gel electrophoresis
Lab Activity: DB Lab 5 Amplification of CO1 by PCR
Due today: DB Labs 1 and 2 Isolating and purifying gDNA
HW:
DB Labs 3 and 4 Confirmation by gel electrophoresis – due Thursday 5/11
Read DB Lab 6 Spin Column to purify CO1 Amplicon
Finish Ch 9.4 Using Chromatography to Study Molecules and do review questions
Do B.O. Pipeline pg 270
Read Ch 9.5 Marketing and Sales, do review questions
Do B.O. Biotech Drugs pg 273
Day 2 Thursday 5/11
In class:
Go over DB Lab 5 Targeted amplification of the COI by PCR
Lab Activity: DB Lab 6: Spin Column to purify CO1 amplicons
Due today: DB Lab 3 and 4 Confirmation of gDNA by gel electrophoresis
HW:
Read DB Lab 7 and 8 Confirmation of COI amplicons by gel electrophoresis
Finish all Biotech Live Ch 9.1-9.3 pg 276-277 and all B.O.s
DB Lab 5 Amplification of CO1 lab report – due on Tues, 5/15
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
05/01/202305/01/2023
Monday – P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 5/2
In class:
Review DB Labs 1 and 2 Purifying gDNA
DB Lab 1 and Lab 2 – Isolate DNA from Bees
Lab Activity: DB Lab 3 and 4 Examining gDNA using Gel Electrophoresis
HW:
DB Labs 1 and 2 Isolate and Purify DNA from Bees – due Thursday 5/4
Read Ch 9.3 Column Chromatography: An Expanded Discussion, do review questions
Do Ch 9.3 B.O. Got Gas pg 268
Read DB Lab 5 Targeted amplification of the COI barcode region from a gDNA template
Day 2 Thursday 5/4
In class:
Go over Purifying DNA from Bees using spin column (chromatography)
Go over Chromatography
Lab Activity: DB Lab 5 Targeted amplification of the COI barcode region from a gDNA template
HW:
Read DB Lab 6 Spin-column purification of COI amplicons
Read Ch 9.4 Using Chromatography to Study Molecules and do review questions
DB Lab 3 and 4 Examining gDNA using Gel Electrophoresis – due on Tue, 5/9
DB Lab 5 Targeted amplification of the COI barcode region from a gDNA template – due Thurs, 5/11
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
04/24/202304/24/2023
Monday – No School
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 4/25
In class:
Intro to DNA Barcoding
DB Lab 1 and Lab 2 – Isolate DNA from Bees
HW:
Read Ch 9.1 Bringing Product to Market, do review questions
Read Intro to DNA Barcoding: DB Lab 1 and Lab 2 – Isolate and purify DNA from Bees
Day 2 Thursday 4/27
In class:
Go over Purifying DNA from Bees
Lab Activity: DB Lab 2 Purify DNA from Bees
HW:
Read DB Lab 3 Casting Gel and DB Lab 4 Gel Electrophoresis
Read Ch 9.2 Using Chromatography to Study Molecules and do review questions
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
04/17/202304/17/2023
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7 Open House
Fri P1-P7 (Minimum Day)
Day 1 Tuesday, 4/18
In class:
ABE Lab 5 Transformation of HB101 with rpGLO
Group Presentation – Ch 8.4 Fermentation and Manufacturing and Ch 8.5 Retrieving Plasmids
HW:
Read Ch 8 Summary and Lab Practices pg 246-248
Do Biotech Live Activity 8.1-8.4 and finish all Ch 5 review questions and Biotech Online
Study for Ch 8 Test – Test on Thurs 4/20
Day 2 Thursday, 4/20
In class:
Ch 8 Test
Lab Activity: Screen for Green Fluorescent Colonies
DNA Barcoding Starts!
HW:
Watch and learn about DNA Barcoding – PPT presentation in GC
Read DB Lab 1 Isolating Genomic DNA from Bees
ABE Lab 5 Transformation – Due on Tues, 4/25
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
04/10/202304/10/2023
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 4/11
In class:
Due today: ABE Lab 2 and 3 Restriction Digest and Ligation of pDrk and pGrn
Go over ABE Lab 4 Confirmation of Restriction Digest of pGRN and pDRK
Grp Presentation: Ch 8.3 After Transformation and do review questions
Lab Activity: ABE green Labs 5 Transformation of HB101 with rpGLO
HW:
Do B.O. pg 238 You can Try This at Home
Read Ch 8.4 Fermentation, Manufacturing, and GMP and do review questions
Due on Thursday: ABE Lab 4 Confirmation of Restriction Digest of pGRN and pDRK
Synthetic Biology Project, Group presentations begin on 4/21
Day 2 Thursday 4/13
In class:
Due today: ABE Lab 4 Confirmation of Restriction Digest of pGRN and pDRK
Go over ABE Lab 5 Transformation of HB101 with rpGLO
Do Biotech Live: Activity 8.1-8.4 pgs 248-250
HW:
Read Ch 8.5 Retrieving Plasmids after Transformation and do review questions
Finish ABE Lab 5 Transformation of HB101 with rpGLO – due Tues 4/17
EP: Transformation and Mini Prep – take notes and take quiz
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
04/03/202304/03/2023
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 4/4
In class:
Group presentation - Ch 8.1 Recombinant Biotechnology
Set up ABE Lab 2 Restriction Digest of pDrk and pGrn and ABE Lab 3 Ligation
Due in two weeks: Synthetic Biology Project, Group presentations begin on 4/21
HW:
Read Ch 8.2 Transforming Cells and do review questions
Do B.O. BACS and YACS pg 228
Read ABE (green) Labs 2 and 3 Restriction Digest and Ligation of pDrk and pGrn
Read ABE Lab 4 Confirmation of Digest of pDrk and pGrn and do prelab notes
ABE Lab 2 Restriction Digest and Ligation of pDrk and pGrn – lab report due on Tue 4/11
Day 2 Thursday, 4/6
In class:
Group presentation – Ch 8.2 Transforming Cells and correct review questions
Lab Activity: ABE Lab 4 Confirmation of Digest of pDrk and pGrn
HW:
Read Ch 8.3 After Transformation and do review questions
Read ABE green Labs 5 Transformation of HB101 with rpGLO
Due in two weeks: Synthetic Biology Project, Group presentations begin on 4/21
ABE Lab 2 Restriction Digest and Ligation of pDrk and pGrn – lab report due on Tue 4/11
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
03/27/202303/27/2023
Mon P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 3/28
Review Ch 7.4 and 7.5
Due today: Lab 7a and 7b Lab reports
Group Presentation – Ch 7.2 pH and Ch 7.3 Buffers, correct review questions
Lab Activity: pH Lab next Tuesday
HW:
Finish Ch 7 review questions, Biotech Live and Biotech Online – due on Thurs 3/30
Read Ch 7 and Lab Practices to get ready for Ch 7 Test
Ch 7 Test on Thurs 3/30
Soda Lab Reports – due on Thurs 3/30
Read ABE Lab 2 Restriction Digest pGrn and pDrk with HindIII and Lab 3 Ligation
Lab Activity - ABE Lab 2 Restriction Digest pGrn and pDrk with HindIII
Day 2 Thursday, 3/30
In class:
Ch 7 Test today
Due today - Ch 7 review questions, Biotech Live and Biotech Online
Due today - Soda Lab Report
Ch 8.1 Overview of Genetic Engineering
Lab Activity: ABE Lab 3 Ligation
HW:
Read Ch 8.1 Overview of Genetic Engineering and do review questions
Read ABE Lab 3 and 4 Ligation and Confirmation of Restriction Digest
ABE Lab 2 and Lab 3 Restriction Digest pGrn and pDrk with HindIII and Ligation – due on Thurs 4/6
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
03/20/202303/20/2023
Monday – Professional Development; no students
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 3/21
In class:
Review Ch 7.1 Spectrophotometers
Group Presentation today: Ch 7.4 and 7.5 Using Spectrophotometers to measure Concentration
Lab Activity: Soda Lab using the Spectrophotometer; create excel file, convert %T to A
Due today: Lab 6g and 6h lab reports
Quiz on Ch 7.1 Spectrophotometer
HW:
Read Ch 7.2 and do review questions
Do B.O pg 203
Finish Labs 7a and 7b – How to use a spectrophotometer – due on Thur 3/23
Day 2 Thursday, 3/23
In class:
Virtual Simulation on Buffer/pH
Review Ch 7.5 Use of Spectrophotometer to measure DNA Concentration
Finish Biotech Live 7.1 and 7.2 pgs 216-217
Read Biotethics pg 217-218: Test Results: who should have access to them?
HW:
Finish Lab 7a and 7b Lab reports due on Tues 3/28
Read Ch 7.3 Buffers and do review questions
Do B.O. pg 208
Finish Biotech Live 7.1 and 7.2 pgs 216-217
Read Biotethics pg 217-218: Test Results: who should have access to them?
Lab Activity: pH Lab next Tuesday
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
03/13/202303/13/2023
HW:
Read Ch 7.4 and 7.5 Use of Spectrophotometers to measure Protein Concentration
Do 7.4 and 7.5 Review questions
Finish Lab 7a and 7b lab reports – due on Tues 3/21
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
03/06/202303/06/2023
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7
Fri P1-P7 (minimum day)
Day 1 Tuesday, 3/7
In class:
Review Lab 6g and 6 h Isolate HRP and Test HRP Activity using TMB
Review Ch 7.1 Spectrophotometer
Lab Activity: Lab 7a and 7b How to Use a Spectrophotometer
HW:
Finish Lab 7a and 7b How to Use a Spectrophotometer
Read Ch 7.4 Use of Spectrophotometers to measure Protein Concentration
Do 7.4 Review questions
Finish Lab 6g and 6h lab reports – due on Thurs 3/9
Finish Lab 7a and 7b lab reports – due on Tues 3/21
Day 2 Thursday, 3/9
In class:
Review Labs 7a and 7b How to use a spectrophotometer
Lab Activity: Soda Lab using the Spectrophotometer; create excel file, convert %T to A
Due today: Lab 6g and 6h lab reports
Quiz on Ch 7.1 Spectrophotometer
HW:
Read Ch 7.4 and 7.5 Use of Spectrophotometers to measure Protein Concentration
Do 7.4 and 7.5 Review questions
Finish Lab 7a and 7b lab reports – due on Tues 3/21
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
02/27/202302/27/2023
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 2/28
In class:
Read Ch 7.1 Spectrophotometer and do review questions pgs 195-197
Do B.O. 7.1 FTIR, pg 200
Read Ch 7.2 and do review questions pgs 200-203
Do B.O. pg 203 Learn how a pH meter electrode works
HW:
Edpuzzle: Spectrophotometer (in the library from 3:30 – 4:30 pm)
Finish Ch 7.1 and 7.2 review questions and B.O. 200 and 203
Read Lab 6h Testing for HRP with TMB – lab on Thurs 3/2
Finish Lab 6d Looking for a new product report – due on Thurs 3/2
Day 2 Thursday, 3/2
In class:
Group presentation on Ch 7. 1 Spectrophotometer
Lab Activity: Lab 6h Testing for HRP with TMB
Due today: Lab 6d Looking for a new product report
HW:
Finish Lab 6h Testing for HRP with TMB – due Thurs 3/9
Read Ch 7.3 Buffers and do review questions
Do B.O. pg 208 How and why proteins are purified from cells
Read Lab 7a and 7b How to Use a Spectrophotometer
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
02/20/202302/20/2023
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 2/21
In class:
Review Ch 6.4 Western Blot and Ch 6.5 Looking for new products
Group Presentation on Ch 6.6 Producing rDNA and correct review questions
Lab Activity -Lab 6d Looking for new product, part 2 – test extract against E.coli
HW:
Finish Biotech Online pg 183 Quality Not and Quantity
Finish Biotech Live Activity 6.1-6.4 – due on Thurs 2/23
Ch 6 Test – Thurs 2/23; Ch 6 packet with review questions, B.O., Biotech Live - due on Thurs 2/23
Day 2 Thursday, 2/23
In class:
Review Ch 6.6 Producing rDNA
Ch 6 Test
Due today: Ch 6 Packet with review questions, B.O., Biotech Live
Lab Activity: Finish Lab 6d - look for anti-microbial activity – due on Thurs 3/2
Read Lab 6g and 6h Isolation and Testing for HRP with TMB Lab – lab on Tues 2/28
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
02/13/202302/13/2023
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 2/14
In class:
Stem Cell Lecture # 5 by Dr. Lu – last day
Stem Cell Debate
HW:
Group presentation on Thurs, 2/16 – 6.4 Western Blot and Ch 6.5 Looking for new products
Do Biotech Online pg 178 Amazon Hide and Seek
Read Lab 6d Looking for new product – lab on Thurs, 2/23
Bring your herbs
Day 2 Thursday, 2/16
In class:
Group presentation – Ch 6.4 Western Blot and Ch 6.5 Looking for new products
Lab Activity -Lab 6d Looking for new product, part 1
HW:
Read Ch 6.6 Producing rDNA and do review questions
Do Biotech Online pg 183 Quality Not and Quantity
Do Biotech Live Activity 6.1-6.4 – due on Thurs 2/23
Group Presentation – Ch 6.6 Producing rDNA and correct review questions – on Tues, 2/21
Ch 6 Test – Thurs 2/23; Ch 6 review questions- due on Thurs 2/23
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
02/06/202302/06/2023
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 2/7
In class:
Stem Cells – Lecture #3 by Dr. Lu
HW:
Stem Cell #3 Blog
Read Ch 6.6 Producing rDNA and do review questions
Do Biotech Online pg 163 Quality Not and Quantity
Day 2 Thursday 2/9
In class:
Stem Cells – Lecture #4
HW:
Stem Cells #4 Blog
Do Biotech Live Activity 6.1-6.4 – due on Thurs 2/14
Group Presentation – Ch 6.6 Producing rDNA and correct review questions (will do on 2/11)
Ch 6 Test – Thurs 2/9; Ch 6 review questions- due on Thurs 2/16
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
01/30/202301/30/2023
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 1/31 (PCC Stem Cell Module Starts today)
In class:
Review Ch 6.3 and go over ELISA Lab
Group presentation – 6.4 Western Blot, Ch 6.5Looking for new products
Due today: Lab 6c Amylase Lab
Lab Activity – Lab 6d Looking for new products, continue on Thurs
HW:
Read Ch 6.6 Producing rDNA and do review questions
Finish ELISA Anti-Heparinv Lab – due on Thurs 2/2
Day 2 Thursday 2/2
In class:
Review Lab 6e Looking for new products
Due today: ELISA Anti-Heparin Lab
Group Presentation – Ch 6.6 Producing rDNA and correct review questions
Lab Activity: continue Lab 6d part 2 - Test plant extract against E. coli
HW:
Do Biotech Live Activity 6.1-6.4 – due on Thurs 2/9
Lab 6d Looking for new products – due on Thurs 2/9
Ch 6 Test – Thurs 2/9; Ch 6 review questions- due on Thurs 2/9
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
01/23/202301/23/2023
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 1/24
In class:
Review Ch 6.3 ELISA and Amylase
Finish Lab 6c Amylase Lab
Due today: Lab 6b Assay for Starch and Sugar
HW:
Read ELISA Lab – Lab on Thursday
Read Ch 6.4 Western Blots and do review questions
Day 2 Thursday, 1/26
In class:
Review Lab 6c Amylase
Group Presentation – Ch 6.4 Western Blot
Lab Activity: Anti-Heparin ELISA Assay
HW:
Read Ch 6.5 Looking for new products
Do B.O. pg 178 Amazon Hide and Seek
Lab 6c Assaying for Amylase Activity – due Tues 2/1
Continue working on Synthetic Biology Project
Bring Herbs for Tuesday and read Lab 6d Looking for Potential Medicine
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp 6 – Ch 7.2, 7.3
Grp 7 – Ch 8.1-8.5
Grp 8 – Ch 9.1-9.5
Grp 1,2,3 – Ch 12
Grp 6,7,8 – Ch 13
01/16/202301/16/2023
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 1/17
In class:
Review Ch 6.1 Potential Biotech Product and Ch 6.2 The use of Assays, correct review questions
Lab Activity: Lab 6b Assay for Starch and Sugar – due Tue 1/24
HW:
Read Ch 6.3 ELISA and do review questions 6.3
Do Biotech Online ELISA Technology in Diagnostic Kits pg 173
Due on Thurs, 1/19 – Rapid ELISA, TRU RSV
Day 2 Thursday, 1/19
In class:
Review Ch 6.2 Assays
Group 1 Presentation - Ch 6.3 ELISA, correct review questions
Due today: Rapid ELISA, TRU RSV
Lab Activity: Lab 6c Assaying for Amylase Activity
HW:
Read ELISA Lab
Finish Lab 6c Amylase Activity – due Thurs 1/26
Edpuzzle – ELISA
Continue working on Synthetic Biology Project
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp 6 – Ch 7.2, 7.3
Grp 7 – Ch 8.1-8.5
Grp 8 – Ch 9.1-9.5
Grp 1,2,3 – Ch 12
Grp 6,7,8 – Ch 13
01/09/202301/09/2023
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, Jan 10
In class:
Review Ch 5 Proteins and PAGE, Polyacrylamide Gel Electrophoresis
Go over Synthetic Biology Project –due in 10 weeks
Assign chapters for group presentations
HW:
Read Ch 6.1 Assays
Do review questions 6.1
Brainstorm ideas for Synthetic Biology Project
Click here: https://biobuilder.org/lab/biology-by-design/?a=student
Day 2 Thursday, Jan 12
In class:
Go over Synthetic Biology – go to www.Biobuilder.org
Ch 6.1 Potential Products
Lab Activity: Rapid ELISA antibody assay
HW:
Read Ch 6.2 Assays
Do review questions 6.2
Rapid ELISA antibody Assay – Lab report due on Thurs, Jan 19
Brainstorm ideas for Synthetic Biology Project
Click here: https://biobuilder.org/lab/biology-by-design/?a=student
Chapter Assignments: Please prepare a Powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp 6 – Ch 7.2, 7.3
Grp 7 – Ch 8.1-8.5
Grp 8 – Ch 9.1-9.5
Grp 1,2,3 – Ch 12
Grp 6,7,8 – Ch 13
12/12/202212/12/2022
Monday P1– P7 regular schedule
T/Th P1, P3, P5, P7
W/F P2, P4, P6, - Final exam P7
Day 1 Tuesday, 12/13
In class:
Due today: Ch 5 Packet
Hands on Activity: Constructing a paper recombinant plasmid
Review for Finals
HW:
Study for Finals, Units 1-4 – Finals on Tuesday
Finish Study Guide for Finals
Day 2 Thursday Dec 15
Final Exam – Chapters 1-5
Good luck on the rest of your finals!
12/05/202212/05/2022
M – Banking Day, P1-P7
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Dec 6
In class:
Due today -– Antibody-Antigen: HCG and iFOBT (Occult Blood) Lab reports
Go over Graphing Data from Catalase Lab – Lab report due Tues 12/13
Correct Ch 5.3-5.4 Protein Analysis
Lab Activity: PAGE – Polyacrylamide Gel Electrophoresis simulation: biomodel.uah.es/en/lab/SDS-PAGE/inicio.htm
Homework:
Study for Ch 5 Test – Test on Thurs 12/9
Read Ch 5.5 Applications of Protein Analysis
Read Ch 5 Summary and Lab Practices pgs 158-159
Finish Catalase Lab – due Tues, 12/13
Thursday, Dec 8
In class:
Go over PAGE – Polyacrylamide Gel Electrophoresis
Do Biotech Live Activity 5.1-5.3 and correct Ch 5.4 Review Questions
Ch 5 Test
Homework:
Complete Ch 5 Packet – due Tues 12/13
Finish Catalase Lab – due Tues, 12/13
Finish PAGE Lab report – due Thurs 12/15
Study for Finals, Ch 1-5 – Finals on Thurs 12/15
11/28/202211/28/2022
M – Banking Day, P1-P7
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Nov 29
In class:
Review Ch 5.1-5.3 Protein Enzymes and correct assignments
Due today -– ABE Lab 7 Isolation of RFP by Column Chromatography
Lab Activity – Antibody-Antigen Test
Homework:
Read Ch 5.1-5.3 more on Proteins
Complete Antibody-Antigen Test lab report – due Tues, 12/6
Thursday, Dec 1
In class:
Review Enzymes and Studying Proteins
Lab Activity – Catalase Lab, parts A-D
Homework:
Graph results of Catalase Lab
Read PAGE Lab (Polyacrylamide Gel Electrophoresis)
Review Ch 5.4 and Study for Ch 5 Test
Ch 5 Test – Thurs, 12/8
11/21/202211/21/2022
Reminder: Finish ABE Lab 7 Isolation of RFP by Column Chromatography – due on Tue 11/29
Read Ch 5.4 Studying Proteins and Ch 5.5 Analyzing Proteins and do review questions
B.O. Protein Sequencers – pg 156
Happy Thanksgiving!
11/14/202211/14/2022
M – Banking Day, P1-P7
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Nov 15
In class:
Presentations on Genetic Disorders from NCBI/BLAST activity
Ch 5.1 and 5.2 Protein Structure and Function
Lab Activity today – ABE Lab 7 Isolations of RFP by Column Chromatograpy; do prelab questions
Quiz on Ch 5.1 Proteins
Homework:
Read Ch 5.3 Protein Catalysts and do review questions
B.O. Catalysts for Better Health pg 151
Read ABE Lab 7 Overnight Cultures of Transformed E.coli; do prelab questions
Read ABE Lab 7 Isolations of RFP by Column Chromatography
ABE Lab 6 Overnight Culture of transformed E.coli cells – due on Thurs 11/17
Thursday, Nov 17
In class:
Go over ABE Lab 7 Isolations of RFP by Column Chromatography
Ch 5.2 and 5.3 Protein Production and Protein Activity; correct review questions
Finish Genetic Disorder presentations
Due today: ABE Lab 6 O/N Culture of transformed E.coli cells
Homework:
Finish ABE Lab 7 Isolation of RFP by Column Chromatography – due on Tue 11/29
Read Ch 5.4 Studying Proteins and Ch 5.5 Analyzing Proteins and do review questions
B.O. Protein Sequencers – pg 156
HAPPY THANKSGIVING!
11/07/202211/07/2022
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7
Friday – Veteran's Day, No School
Tuesday, Nov 8
In class:
Review ABE Lab 4 and 5 – Confirmation of Digest and Transformation
Lab Activity today – ABE Lab 6 Set up Overnight Culture of Transformed Cells; Screen for Transformed, red cells
Quiz on ABE Lab 4, 5 Confirmation, and Transformation
Homework:
ABE ABE Lab 4, 5 Confirmation, Transformation due Thursday, 11/10
Read Ch 5.1 Protein structure and function and do review questions
B.O. Antibody Producing Companies
Read ABE Lab 6 Overnight Cultures of Transformed E.coli; do prelab questions
Thursday, Nov 10
In class:
Go over ABE Lab 6 Overnight Culture
Lab Activity: ABE Lab 6 Set up Overnight Culture.
Ch 5.2 Protein Production and Protein Activity
Due today: ABE Lab 4 Confirmation of Restriction Digest
Homework:
Read ABE Lab 7 Isolation of RFP by Column Chromatography
Read Ch 5.2 Protein Production
B.O. Couch Potatoes pg 146
10/31/202210/31/2022
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7
F P1-P7 Shakeout Assembly Schedule
Tuesday, Nov 1
In class:
Review ABE Lab 4 – Confirmation of Restriction Digest of pAra and pKan-R and Ligation
Hands-on Activity – HASPI Diagnosing Genetic Disease Through Gel Electrophoresis
ABE Labs 2 and 3 lab reports due on Thursday, 11/4
Quiz on ABE Labs 1 -3
Homework:
Read ABE Lab 5 Transformation of E.coli with pAra-R
Finish Activity on Diagnosing Genetic Disease Through Gel Electrophoresis – Due on Tue 11/8
Complete ABE Labs 2 and 3 Ligation – Due on Thurs 11/4
ABE Lab 4 Confirmation – Due Tue 11/8
Finish Ch 4 Packet – due Thurs 11/4
Thursday, Nov 3
In class:
Go over Bacterial Transformation
Lab Activity: ABE Lab 5 Transformation of E.coli with pAra-R
Activity: Bioinformatics NCBI and Blast
Due today: ABE Labs 2 and 3 lab reports and Ch 4 packet
Quiz on Restriction digest and Gel Electrophoresis
Homework:
Read ABE Lab 6 Overnight Culture of Transformed E.coli
Finish ABE Labs 4 report – due Thurs 11/11
Finish Activity on Diagnosing Genetic Disease Through Gel Electrophoresis – Due on Tue 11/8
Finish : Bioinformatics NCBI and Blast – due on Tues, Nov 8
10/24/202210/24/2022
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7
Tuesday, Oct 25
In class:
Go over Gel Electrophoresis
Correct 4.4 review questions; Ch 4 Test on Thursday, 10/27
Set up ABE Lab 3 Ligation of pAra and pKan
Do/Finish CTE Modules 2 & 3
Due today: ABE Lab 1 Gel Electrophoresis
Homework:
Read ABE Lab 3 Ligation and Lab 4 Confirmation of pAra and pKan
Finish Biotech Live 4.1-4.6
Study for Ch 4 Test – Test on Thursday, 10/27
Thursday, Oct 27
In class:
Ch 4 Test
Go over ABE Lab 3 Ligation and Lab 4 Confirmation of pAra and pKan
Lab 4: Lab 4 Confirmation of pAra and pKan by Gel Electrophoresis
Homework:
Due: ABE Lab 4 Confirmation of Restriction Digest by Gel Electrophoresis
Finish ABE Labs 2 and 3 report – due Thursday, 11/3
Finish Ch 4 Packet – Review Questions Ch 4.1-4.4, Biotech Online 4.1-4.4, Biotech Live 4.1-4.6 – due on Thursday, 11/3
10/17/202210/17/2022
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Oct 18
In class:
Go over 4.3 DNA Structure and Isolation of DNA
Quiz on Ch 4.1-4.3
Lab Activity: ABE Lab 1 Gel Electrophoresis
Due: Strawberry DNA Isolation Lab
Homework:
Read Ch 4.4 Gel Electrophoresis and do review Questions
Finish ABE Lab 1 Prelab Notes
Do B.O. Chop and Go Electrophoresis pg 124
Do Ch 4 Biotech Live 4.1-4.2
ABE Lab 1 Gel Electrophoresis – due on Tuesday 10/26
Thursday, Oct 20
In class:
Go over Ch 4.4 Gel Electrophoresis
Quiz on Gel Electrophoresis
Homework:
Do Biotech Online Chop and Go pg 124
Finish ABE Lab 1 Gel Electrophoresis – due on Tuesday 10/26
Read ABE Lab 2 Restriction Digest of pKan and pARA
Read Ch 4 Summary and Lab Practices pgs 126-127
Do Ch 4 Biotech Live 4.4-4.6
Ch 4 Test on Thursday, 10/28
10/10/202210/10/2022
M P1, P3, P5, P7
T/Th P2, P4, P6, P7
W PSAT 8:30 am - 12:57 pm
F P1-P7 Minimum Day
Tuesday, Oct 11
In class:
Ch 4.1 DNA Structure
Lab Activity: Isolation of Strawberry DNA
Due today: Ch 3 Packet and Math Problems; packet should include review questions, Biotech Online, and Biotech Live
Homework:
Read Ch 4.2 Sources of DNA, do review questions
Do Biotech Online Know your Genome pg 112, Baldness Gene pg 118
Download Amgen Lab Manual
Strawberry DNA Lab – due on Tuesday, 10/18
Thursday, Oct 13
In class:
3 pm: Special Guest: Ms. Samantha Male – presentation on her own project on Stem Cells
Ch 4.2 Sources of DNA, correct review questions
Activity: Cast a Gel for Gel Electrophoresis; make 1x SB buffer and 1% agarose gel
Homework:
Read Ch 4.3 Isolating and Manipulating DNA
Do Biotech Online Recombinant Pharmaceuticals and Therapies, pgs 118-119
Read Lab 1 Amgen Lab Manual
Strawberry DNA Lab – due on Tuesday, 10/18
10/03/202210/03/2022
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Oct 4
In class:
Correct Ch 3.6 review questions and Math Problems
Ch 3 Test on Thursday
Due today: Pipetting Accuracy Lab, parts 1 and 2
Classwork: Biotech Live – Activity 3.1-3.4 pgs 95-97 (work on Chromebook)
Homework:
Study for Ch 3 Test – Test on Thursday
Finish Biotech Live – Activity 3.1-3.4 pgs 95-97 and finish Math problems
Micro-pipetting Lab with colored dyes – due Thurs, 10/6
Thursday, Oct 6
In class:
Ch 3 Test
Continue with Biotech Live – Activity 3.5-3.6 pgs 98-100
Go over Math/MW of compounds
Activity: more practice on micro-pipetting
Homework:
Read Ch 4.1 Studying DNA and do review questions
Read Lab 1 Amgen Lab Manual
Bring Strawberries for Tuesday
Date Assigned: 09/19/2022
Date Due: 09/19/2022
M P1-P7 (Banking Day)
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Sept 20
In class:
Correct all Ch 2.4 and 2.5 review questions
Go over Bacteria Lab and Gram Stain
Lab Activity – Gram Stain and use microscope to view bacteria
Homework:
Ch 2 Test on Thursday and Ch 2 packet due on Thursday
Read Ch 3.1-3.2 pgs 71-82 and do review questions
Do B.O. pg 74 on Metrics Conversion
Finish Bacteria Lab– due on Tuesday, 9/27
Microscope Lab – due on Thursday, 9/22
Thursday, Sept 22
In class:
Ch 2 Test and Ch 2 packet due today
Go over Ch 3.1-3.2 review questions and math problems
Go over Gram Stain Lab
Lab Activity – Pipetting Accuracy
Due today: Microscope Lab
Homework:
Read Ch 3.3-3.4 and do review questions
Do Math Problems
Grams Stain Lab – due on Thurs, 9/29
Pipetting Lab due Thurs, 9/29
09/12/202209/12/2022
M P1-P7 Banking Day
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, Sept 13
In class:
Go over Organic Lab and complete Microscope Lab
Correct Ch 2.1-2.3 review questions
Due today: Cheese Lab
Lab Activity – Bacteria Lab: collect bacteria, streak media plate, test against antibiotics
Homework:
Read Ch 2.4 New Biotechnology and do 2.4 review questions
Do B.O. pgs 43 Kimchi, 46 Right Tool, 52 Molecular Models, and 82 Biotech Products
Finish Cheese Lab with Chymax and Organic Lab - both are due on Thursday, 9/15
Microscope Lab – due on Thursday, 9/22
Ch 2 Test on Thursday, 9/22
Thursday, Sept 15
In class:
Due today: Cheese Lab with Chymax and Organic Lab
Correct Ch 2.4 review questions
Go over Bacteria Lab and Gram Stain
Lab Activity: Gram Staining of bacteria
Homework:
Finish Bacteria Lab – due on Thursday, 9/22
Do Biotech Live Activity 2.1-2.3 pg 86
Read Ch 2 Summary and Lab Practices pg 81-86
Ch 2 Test on Thursday, 9/22
09/05/202209/05/2022
T/Th P1, P3, P5, P7
W P2, P4, P6, P7
F P1-P7 (Minimum Day)
Tuesday, Sept 6
In class:
Review Ch 1.3-1.6 and correct review answers
Discuss careers and Biotethics in Biotechnology- watch video on Biochemist
Check cheeseball – make final observation: color, texture, smell
Lab Activity: Organic Lab – lab report due on
Homework:
Read Summary and Lab Practices on pgs 30-31 to review for Ch 1 Test
Study for Ch 1 Test – Test on Thursday, Sept 8
Cheese Lab is due on Tuesday, Sept. 13
Cheese Lab with Chymax – due on Thursday, Sept 15
Read Ch 2.1, do review question and Label parts of Microscope (worksheet) due on Thursday 9/8
Remember to copy review questions and answer in complete sentences on a separate sheet
Thursday, Sept 8
In class:
Ch 1 Test in class on Quizziz
Go over Ch 2.1 Review Questions, Organic Lab, and Microscope worksheet
Lab Activity: Microscope Lab and do cells worksheet on structures and function
Homework:
Read Ch 2.2-2.3, do review questions; remember to copy questions and answer in complete sentences
Complete cell structure and function worksheet
Finish Cheese Lab – due on Tues, 9/13
Cheese Lab with Chymax – due on Thurs, Sept 15
Finish Organic Lab – due on Thurs, 9/15
08/29/202208/29/2022
M - P1-P7 (Banking Day)
T/Th - P1, P3, P5, P7
W/F - P2, P4, P6, P7
Tuesday, August 30
In Class:
What is Scientific Method?
Correct Ch 1.2-1.3 review questions
Lab Activity: Cheese Lab, make first observation, record in your notebook
Biotech Live: Activity 1 What is Biotechnology? pgs 32-33
Activity 2 Business Side pgs 33-34
Bleach Lab due today– Did you send to intern to check for content/format?
HW:
Read ED Ch. 1.4 and review questions pgs 19-23 ED
Do B.O. Hot Jobs pg 26
Extra Credit: make cheese at home; DIY on foodwishes.com
Thursday, Sept 1
In Class:
Cheese Lab - Make 2nd observation, record in your notebook ready for cheese cloth?
Discuss Careers in Biotech
Do Biotech Live Activity 1.4 How Biotech Industry Improving Human Life? pg 35
Correct Ch 1.4 review questions
HW:
Read ED Ch 1.5-1.6 and do review questions pgs 23-29 ED
Do B.O. Code of Business Conduct pg 29 ED
Read Summary and Lab Practices pgs 30-31
Please share your lab report with your intern to get it checked before turning it in to me.
Biotech Interns: Here are your interns:
Kayleen Yang kyan2348@stu.gusd.net A-Cabrera
Rosalia Park - rpar7075@stu.gusd.net
Rachel Lee rlee5733@stu.gusd.net Chang to H
Claire Yang - cyan6089@stu.gusd.net
Nate Jaramillo njar6348@stu.gusd.net J to L
Michelle Chung - mchu2590@stu.gusd.net
Milena and Astgik mjag6984@stu.gusd.net P-V asha9484@stu.gusd.net
Nathan Pang - npan1489@stu.gusd.net
These are our presidents and will help if your intern is too busy. So don't hesitate to send it to them.
Rosalia Park - rpar7075@stu.gusd.net Nate Jaramillo njar6348@stu.gusd.net
Claire Yang - cyan6089@stu.gusd.net Rachel Lee rlee5733@stu.gusd.net
Michelle Chung - mchu2590@stu.gusd.net
Nathan Pang - npan1489@stu.gusd.net
08/22/202208/22/2022
T/Ths – 2:40-4:50 pm
M - P1-P7 (Banking Day)
T/Th - P1, P3, P5, P7
W/F - P2, P4, P6, P7
Tuesday, Aug 23
In class:
What is Biotech?
Go over How to maintain a Scientific Notebook in Biotech
Lab Activity: Bleach Lab pgs 19-21 TB
HW:
Read Ch 1.1-1.2 and do review questions Ch 1.1-1.2 in ED - write questions and answers on paper with black ink
Read Cheese Lab before Thursday and bring whole milk and buttermilk on Thursday
Do Biotech Online, B.O., pg 13 Glo Fish
Day 2 Thursday, Aug 25
In class:
Complete and make final observations for Bleach Lab, lab report due on Th 9/1
Review How to Notebook and how to write an effective conclusion
Lab Activity: Cheese Lab
HW:
Read Ch 1.3 pgs 14-18 and do review questions 1.3 ED
Do Biotech Online (B.O.) pg 18 Genentech
Finish Bleach Lab report in Google Docs pgs 19-21 ED; lab report due on Th 9/1
08/15/202208/15/2022
Day 1 Wednesday, August 17
In class:
Rules and Policies
Safety Rules
HW:
Sign up for Google Classroom, Quizizz
Study for Safety Test – Test on Thursday
Wear shoes to class – Always!
Day 2 Thursday, August 18
In class:
Safety Test
What is Biotechnology
HW:
Read Ch 1.1 Biotechnology ED (Ellyn Daugherty textbook)
Read Bleach Lab pgs 19-21 ED, Lab on Tuesday
Wear shoes, = please
06/06/202206/06/2022
M P1, P3, P5, P7
T P2, P4, P6, P7, Senior Finals
W P1, P3, P5, P7
Th P1- P6, Finals in P7
F P1, P3, P5
Day 1 Tuesday, 6/7
In class:
Lab Activity: DB Lab 6 Spin Column to purify CO1 Amplicon
Group Presentation on Ch 13.3-13.4 Applications of PCR
HW:
Read DB Lab 8 Gel Electrophoresis to confirm CO1 Amplicons
Study for Finals, Ch 6-9, 12, 13 – Finals on Thursday, 6/9
Day 2 Thursday 6/9
In class:
Lab Activity: DB Lab 8 Gel Electrophoresis to confirm CO1 Amplicons
Finals today! - Ch 6-9, 12, 13
05/30/202205/30/2022
Monday – Memorial Day Holiday – No School
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 5/31
In class:
Lab Activity: DB Lab 4 Gel Electrophoresis
Go over DB Lab 5 Amplifying CO1 by PCR, Polymerase Chain Reaction
Group Presentation on Ch 12.5 Recent Advances
HW:
Read DB Lab 6 Spin Column to Isolate CO1 Amplicons
Read Ch 13.1-13.2 Making DNA Molecules, do review questions
Study for Finals – Finals on Friday, 6/10
Day 2 Thursday 6/2
In class:
Lab Activity: DB Lab 6 Spin Column to Isolate CO1 Amplicons
Group Presentation on Ch 13.3-13.5 Polymerase Chain Reaction
HW:
Read DB Lab 7 Confirmation of CO1 Amplicons
Study for Finals – Finals on Friday, 6/10
05/23/202205/23/2022
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 5/24
In class:
Group Presentation – Ch 12.4 Pharmaceuticals
Lab Activity: DB Lab 2 Isolating Genomic DNA and Lab 2 Casting Gel
HW:
Read DB Lab 3 Casting Gel and DB Lab 4 Gel Electrophoresis
Do B.O. Getting Sick pg 354
Do B.O. Eat Veggies pg 355
Day 2 Thursday 5/26
In class:
Lab Activity: DB Lab 4 Gel Electrophoresis
HW:
Read DB Lab 5 and Lab 6 Amplifying CO1 and Confirmation
Read Ch 12.5 Recent Advances in Medical Biotechnologies
Do B.O. Medicine for you pg 357
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp8 – Ch 12
Grp9 – Ch 13
05/16/202205/16/2022
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 5/17
In class:
Group Presentation - Ch 9.5 Marketing and Sales
Group Presentation – Ch 12.1 Drug Discoveries
DNA Barcoding
HW:
Read Ch 12.2 Creating Pharmaceuticals and do review questions
Read Intro to DNA Barcoding: DB Lab 1 and Lab 2 – Isolate DNA from Bees
Day 2 Thursday 5/19
In class:
Group Presentation - Ch 12.2 Creating Pharmaceuticals
Lab Activity: DB Lab 2 Isolate DNA from Bees
HW:
Read DB Lab 3 Casting Gel and DB Lab 4 Gel Electrophoresis
Read Ch 12.3 Creating Pharmaceuticals through Peptide Synthesis and do review questions
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp8 – Ch 12
Grp9 – Ch 13
05/09/202205/09/2022
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 5/10
In class:
Check Protein Concentration of isolated RFP
Review Column Chromatography
Column Chromatography to isolate RFP lab report – due on today
Group Presentation: Ch 9.4 Product Quality – correct HW
Lab Activity: Check protein concentration of isolated RFP
Ch 9 Test on Thursday
HW:
Review Ch 9.5 and finish Ch 9.5 review questions
Read Ch 9 Summary and Lab Practices pgs 274-275
Do Biotech Live: Activity 9.1 Protein Manufacturing and 9.2 Setting Standard
Day 2 Thursday 5/12
In class:
Group Presentation: Ch 9.5 Marketing and Sales and correct review questions
Do Biotech Live: Activity 9.3 Reading Annual Reports
Read Bioethics Part I pg 278 and Part II pg 27
Ch 9 Test – Today, Thursday
HW:
Ch 9 Packet – due on Tuesday 5/17
Read Ch 12.1-12.2 Drug Discoveries pg 343-348 and do review questions
B.O. pg 344 New Medicines
B.O. pg 345 Penicillin
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
05/02/202205/02/2022
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 5/3
In class:
Due today: Mini Prep Lab Report
Review Mini prep, check for DNA
Lab Activity: Column Chromatography to isolate RFP
HW:
Review Ch 9.3 more on Column Chromatography and finish 9.3 review questions
Do Ch 9.3 B.O. pg 268 Got Gas
Read Ch 9.4 Product Quality and do review questions
Do Ch 9.4 B.O. pg 279 Products in the Pipeline
Day 2 Thursday 5/5
In class:
Group Presentation: Ch 9.3 more on Column Chromatography
Group Presentation: Ch 9.4 Product Quality
Check Protein Concentration of isolated RFP
Quiz on Ch 9.3-9.4 today
HW:
Read Ch 9.5 Marketing and Sales and do review questions
Do Ch 9.5 B.O. Biotech Drugs pg 273
Column Chromatography lab report – due on Tues 5/10
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
04/25/202204/25/2022
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 4/26
In class:
Review Mini/Maxi Prep
Group presentation – Ch 9.1 Harvesting a Protein Product
Lab Activity: Mini Prep using BioRad kit; also set up an overnight culture for column chromatography
HW:
Read Ch 9.2 Column Chromatograpy and do review questions pgs 253-258
EP: Column Chromatography – due on Thurs 4/28 @ 8 am
Day 2 Thursday 4/28
In class:
Review Ch 9.1 and correct review questions
Group presentation Ch 9.2 Column Chromatography
Lab Activity: Column Chromatography to isolate a protein
HW:
Read Ch 9.3 more on Column Chromatography
EP: HPLC due Tues 5/4
Mini-Prep lab report – due on Tues 5/4
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
04/18/202204/18/2022
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 4/19
In class:
Go over Karyotype
Stem Cell Module #5 by Dr. Karol Lu – Day #5 @ 2:45 pm
Final Activity: Stem Cell Debate
HW:
Study for Ch 8 Test – Test on Tues 4/20
Read Ch 8 Summary and Lab Practices pgs 246-248
Do Thinking Like a Biotechnician pg 248
Do Biotech Live Activity – 8.1-8.5
Ch 8 packet due on Wednesday
Day 2 Wednesday 4/20
In class:
Group presentation Ch 8.5 and correct Ch 8.5 review questions
Ch 8 Test and Ch 8 packet due today
HW:
Read Ch 9.1 Bringing product to market and do review questions
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
04/11/202204/11/2022
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 4/12
In class:
Correct Ch 8.5 Retrieving Plasmids and do review questions
Stem Cell Module #3 by Dr. Karol Lu – Day #3 @ 2:45 pm
HW:
Stem Cells HW #3 – Study for Stem Cells Quiz
Study for Ch 8 Test – Test on Tues 4/19
Read Ch 8 Summary and Lab Practices pgs 246-248
Do Thinking Like a Biotechnician pg 248
Day 2 Thursday 4/14
In class:
Stem Cell Module #4 by Dr. Karol Lu – Day #3 @ 2:45 pm
HW:
Stem Cells HW #3 – Study for Stem Cells Quiz
Study for Ch 8 Test – Test on Tues 4/19
Do Biotech Live Activity 8.1-8.4 pgs 248-250
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
04/04/202204/04/2022
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 4/5
In class:
Correct Ch 8.2-8.3 Transformation and After Transformation assignments
Quiz on Ch 8.2-8.3 Transformation and After Transformation
Go over Lab 4 Confirmation of Restriction Digest
Stem Cell Module #1 by Dr. Karol Lu from PCC CIRM program
HW:
Read Ch 8.5 Retrieving Plasmids and do review questions
Stem Cells HW #1
Ch 8 Test on Tues, 4/12
Day 2 Thursday 4/7
In class:
Review Ch 8.4 and 8.5 Fermentation and correct assignments
Stem Cell Module #2 by Dr. Karol Lu from PCC CIRM program
HW:
Read Ch 8 Summary and Lab Practices pg 246-248
Do Biotech Live Activity 8.1-8.4
Stem Cells HW #2
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
03/28/202203/28/2022
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 3/29
In class:
Go over ABE Labs 2 and 3 Restriction Digest and Ligation of pDrk and pGrn
Group presentation - Ch 8.2 Transformation
Do prelab notes ABE Lab 5
Lab Activity: ABE Lab 5 Transformation
Due today: Synthetic Biology Project, Group presentations begin
HW:
Review Ch 8.3 After Transformation
Finish ABE Labs 2 and 3 – due Thurs 3/31
Read Amgen (ABE) Manual green Labs 4 and do pre lab notes
Day 2 Thursday, 3/31
In class:
Due: ABE Labs 2 and 3 and go over ABE Lab 5 Transformation and screen for green glowing cells
Set up ABE green Lab 4 Confirmation of Digest
Group presentation: Ch 8.3 After Transformation
HW:
Read Ch 8.4 After Transformation and do review questions
Finish ABE green Labs 4 and 5 Confirmation of Digest and Transformation lab report – due Thurs 4/7
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
03/21/202203/21/2022
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 3/22
In class:
Group presentation - Ch 8.1 Recombinant Biotechnology
Set up Restriction Digest of pDrk and pGrn
Due today: Synthetic Biology Project, Group presentations begin
HW:
Read Ch 8.2 Transforming Cells and do review questions
Do B.O. BACS and YACS pg 228
Read Amgen (ABE) Manual green Labs 2 and 3 Restriction Digest and Ligation of pDrk and pGrn
Day 2 Wednesday, 3/23
In class:
More group presentation Synthetic Biology Project
Set up ABE green Lab 3 Ligation to construct rpGlo.
HW:
Read Ch 8.3 After Transformation and do review questions
Read ABE green Labs 4 and 5 Confirmation of Digest and Transformation
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
03/14/202203/14/2022
Just a friendly remember:
Finish Synthetic Biology Project – due on Tues 3/22
Read Ch 8.1 Production of Recombinant Biotech Prod and do review questions - due on Tues 3/22
Do B.O. Genetic Engineering pg 226 – due on Tues 3/22
Group Presentation on Ch 8.1 – on Tuesday 3/22
HAVE A GREAT SPRING BREAK!
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
HAVE A GREAT SPRING BREAK!
03/07/202203/07/2022
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7
Fri P2, P4, P6, P7
Day 1 Tuesday, 3/8
In class:
Due today: pH Lab
Review Ch 7.2 and pH
Group presentation Ch 7.3 Buffers correct 7.3 review questions
CW: Biotech Live: Do Activity 7.1-7.3 pg 216-277
HW:
Review Ch 7.1-7.5
Read Ch 7 Summary and Lab Practices pgs 214-216
Finish Biotech Live: Do Activity 7.1-7.3 pg 216-277
Ch 7 Test on Thursday 3/10
Day 2 Thursday, 3/10
In class:
Ch 7 Test
Work on Synthetic Biology Project – due on Tues 3/22
HW:
Finish Synthetic Biology Project – due on Tues 3/22
Read Ch 8.1 Production of Recombinant Biotech Prod and do review questions
Do B.O. Genetic Engineering pg 226
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
02/28/202202/28/2022
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 3/1
In class:
Due today: Soda Lab
Group presentation: Ch 7.5 Use of Spectrophotometer to measure DNA Concentration
Activity: Virtual Simulation on Buffer/pH
HW:
Read Ch 7.2 and do review questions
Do B.O. pg 203 Learn how a pH meter electrode works
Due on Tuesday, 3/8 – Virtual Simulation on Buffer/pH
Day 2 Thursday, 3/3
In class:
Review Ch 7.5 Use of Spectrophotometer to measure DNA Concentration
Group presentation on Ch 7. 2 Buffers/pH
Lab Activity: pH Lab
HW:
Finish pH Lab report due on Tues 3/10
Read Ch 7.3 Buffers and do review questions
Do B.O. pg 208 How and why proteins are purified from cells
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
02/21/202202/21/2022
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 2/22
In class:
Due today: Lab 6g and 6h Isolation and Testing for HRP with TMB Lab report due today
Review Lab 7a and 7b How to Use a Spectrophotometer
Lab Activity: Soda Lab using the Spectrophotometer; create excel file, convert %T to A
Quiz on Ch 7.1 Spectrophotometer
HW:
Finish Lab 7a and 7b How to Use a Spectrophotometer lab reports – due on Thurs 2/24
Soda Lab report due on Tues 3/1
Review Ch 7.4 and finish review questions
Day 2 Thursday, 2/24
In class:
Review Lab Activity: Soda Lab
Group presentation on Ch 7. 4 Using Spectrophotometer to find concentration of protein
Due today Lab Activities: Lab 7a and 7b – How to Use a Spectrophotometer
Lab Activity: Virtual Simulation on Using Spectrophotometer to measure concentration of molecules
HW:
Finish Soda Lab report due on Tues 3/1
Read Ch 7.5 Use of Spectrophotometers to DNA and do 7.5 Review questions
Finish Virtual Simulaltion on Spectrophotometer – due on Tue 3/1
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
02/14/202202/14/2022
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 2/15
In class:
Review Lab 6d Looking for new product; Lab report due today
Lab Activity 6g and 6 h Isolate HRP and Test HRP Activity using TMB
HW:
Read Ch 7.1 Spectrophotometer and do review questions
Do B.O. 7.1 FTIR, pg 200
Lab 6g and 6h lab reports – due on Tue 2/22
Read Lab 7a and 7b How to Use a Spectrophotometer
Day 2 Thursday, 2/17
In class:
Review Lab Activity: Lab 6g Isolate HRP
Group presentation on Ch 7.1 Spectrophotometers
Lab Activities: Lab 7a and 7b – How to Use a Spectrophotometer
HW:
Read Ch 7.4 Use of Spectrophotometers to measure Protein Concentration
Do 7.4 Review questions
Finish Lab 6g and 6h lab reports – due on Tue 2/22
Finish Lab 7a and 7b lab reports – due on Thurs 2/24
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
02/07/202202/07/2022
M/Th P1, P3, P5, P7
W P1, P3, P5, P7 (Banking Day)
T/F P2, P4, P6, P7
Day 1 Tuesday, 2/8
In class:
Review Ch 6.6 Production of recombinant protein
Lab Activity – Lab 6d Looking for new products, part 2, test extract against E.coli
HW:
Read Ch 6 Summary pgs 184-185, due on Thurs 2/3
Do Lab Practices pgs 185, due on Thurs 2/3
Finish Biotech Live Activity 6.1-6.4 – due on Thurs 2/3
Ch 6 Review questions - due on Thurs 2/3
Ch 6 Test – Thurs 2/10
Day 2 Thursday, 2/10
In class:
Ch 6 Test
Lab Activity: continue Lab 6d part 2 – check for zone of inhibition
Lab Activity: Lab 6g Isolate HRP
HW:
Read Lab 6g and 6 h Isolating HRP and Testing HRP using TMB
Finish Lab 6d New Products Lab Report – due on Tues 2/15
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
01/31/202201/31/2022
M/W P1, P3, P5, P7
T/Th P2, P4, P6, P7 (Open House)
F P1, P3, P5, P7
Day 1 Tuesday, 2/1
In class:
Review Ch 6.3 and 6.4 Western Blot and go over ELISA Lab
Group presentation – Ch 6.5 Looking for new products
Due today: Lab 6c Amylase Lab
Lab Activity – Lab 6d Looking for new products, continue on Thurs
HW:
Read Ch 6.6 Producing rDNA and do review questions
Finish ELISA Lab – due on Thurs 2/3
Day 2 Thursday, 2/3
In class:
Review Lab 6e Looking for new products
Due today: ELISA Lab
Group Presentation – Ch 6.6 Producing rDNA and correct review questions
Lab Activity: continue Lab 6d part 2 - Test plant extract against E.coli
HW:
Do Biotech Live Activity 6.1-6.4 – due on Thurs 2/10
Lab 6d Looking for new products – due on Thurs 2/10
Ch 6 Test – Thurs 2/10; Ch 6 review questions- due on Thurs 2/10
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
01/24/202201/24/2022
M/T P1, P3, P5, P7
W Banking Day P1-P7
Th/F P2, P4, P6, P7
Day 1 Tuesday, 1/25
In class:
Review Ch 6.3 ELISA and Amylase
Finish Lab 6c Amylase Lab
Due today: Lab 6b Assay for Starch and Sugar
HW:
Read ELISA Lab – Lab on Thursday
Read Ch 6.4 Western Blots and do review questions
Day 2 Thursday, 1/27
In class:
Review Lab 6c Amylase
Group Presentation – Ch 6.4 Western Blot
Lab Activity: Anti-Heparin ELISA Assay
HW:
Read Ch 6.5 Looking for new products
Do B.O. pg 178 Amazon Hide and Seek
Lab 6c Assaying for Amylase Activity – due Tues 2/1
Continue working on Synthetic Biology Project
Bring Herbs for Tuesday and read Lab 6d Looking for Potential Medicine
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
01/17/202201/17/2022
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Day 1 Tuesday, 1/18
In class:
Review Ch 6.1 Potential Biotech Product
Group 1 Presentation – Ch 6.2 The use of Assays, correct review questions
Due today: PAGE gel report
Lab Activity: Lab 6b Assay for Starch and Sugar
HW:
Read Ch 6.3 ELISA and do review questions 6.3
Do Biotech Online ELISA Technology in Diagnostic Kits pg 173
Due on Thurs – Rapid ELISA, iFOBT
Day 2 Thursday, 1/20
In class:
Review Ch 6.2 Assays
Group 1 Presentation - Ch 6.3 ELISA, correct review questions
Due today: Rapid ELISA, iFOBT report
Lab Activity: Lab 6c Assaying for Amylase Activity
HW:
Read ELISA Lab
Finish Lab 6c Amylase Activity – due Thurs 1/27
Edpuzzle – ELISA
Continue working on Synthetic Biology Project
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
01/10/202201/10/2022
M/Th P1, P3, P5, P7
W P1-P7 Banking Day
T/F P2, P4, P6, P7
Day 1 Tuesday, Jan 11
In class:
Review Ch 5 Proteins and PAGE, Polyacrylamide Gel Electrophoresis – lab report due on Tues, 1/18
Go over Synthetic Biology Project –due in 10 weeks
Assign chapters for group presentations
HW:
Read Ch 6.1 Assays
Do review questions 6.1
Brainstorm ideas for Synthetic Biology Project
Day 2 Thursday, Jan 13
In class:
Go over Synthetic Biology – go to www.Biobuilder.org
Ch 6.1 Potential Products
Lab Activity: Rapid ELISA antibody assay
HW:
Read Ch 6.2 Assays
Do review questions 6.2
Finish PAGE Report, due Tues 1/18
Chapter Assignments: Please prepare a powerpoint presentation for each section.
Grp1 - Ch 6.1-6.3
Grp2 – Ch 6.4-6.6
Grp3 – Ch 7.1, 7.4, 7.5
Grp4 – Ch 7.2, 7.3
Grp6 – Ch 8.1-8.5
Grp7 – Ch 9
Grp8 – Ch 12
Grp9 – Ch 13
12/13/202112/13/2021
Monday P1, P3, P5, P7 – regular schedule
Tuesday P1-P7 Final Exam in P7 only
Wednesday P1, P3, P5 Final Exam
Thursday P2, P4, P6 Final Exam
Friday No School Winter Break Begins
Day 1 Monday, Dec 13 P7 2:39 pm - 3:29 pm
In class:
Review for Finals
HW:
Study for Finals, Units 1-4 – Finals on Thursday
Finish Study Guide for Finals
Day 2 Tuesday, Dec 14 P7 1:33 pm – 3:29 pm
Final Exam – Chapters 1-5
Enjoy your Winter Break!
12/06/202112/06/2021
M/Th P1, P3, P5, P7
W – Banking Day, P1-P7
T/F P2, P4, P6, P7
Tuesday, Dec 7
In class:
Due today -– Antibody-Antigen Flu Test
Go over Catalase Lab – Lab report due Thurs 12/9
Lab Activity: PAGE – Polyacrylamide Gel Electrophoresis
Correct Ch 5.4 Protein Analysis
Homework:
Study for Ch 5 Test – Test on Thurs 12/9
Complete Catalase Lab – due Thurs, 12/9
Thursday, Dec 9
In class:
Finish PAGE – Polyacrylamide Gel Electrophoresis
Due today – Catalase Lab report
Ch 5 Test
Homework:
Finish PAGE Lab report – due Tues, 12/14
Study for Finals, Ch 1-5 – Finals on Tues 12/14
11/29/202111/29/2021
M/Th P1, P3, P5, P7
W – Banking Day, P1-P7
T/F P2, P4, P6, P7
Tuesday, Nov 30
In class:
Review Ch 5 Protein Enzymes and correct assignments
Due today -– ABE Lab 6 and ABE Lab 7 Isolation of RFP by Column Chromatography
Lab Activity – Antibody-Antigen Test
Homework:
Read Ch 5.1-5.3 more on Proteins
Complete Antibody-Antigen Test lab report – due Tues, 12/6
Thursday, Dec 2
In class:
Review Enzymes and Studying Proteins
Lab Activity – Catalase Lab, parts A-D
Homework:
Graph results of Catalase Lab
Read PAGE Lab (Polyacrylamide Gel Electrophoresis)
Review Ch 5.4 and Study for Ch 5 Test
Ch 5 Test – Thurs, 12/9
11/22/202111/22/2021
Reminder: Complete ABE Lab 6 Overnight Culture and Lab 7 Isolation of MFP by Column Chromatography – due on 11/30
Happy Thanksgiving!
11/15/202111/15/2021
M/Th P1, P3, P5, P7
W – Banking Day, P1-P7
T/F P2, P4, P6, P7
Tuesday, Nov 16
In class:
Presentations on Genetic Disorders
Ch 5.1 Protein Structure and Function
Lab Activity today – ABE Lab 6 Set up Overnight Culture of Transformed Cells
Quiz on Ch 5.1 Proteins
Homework:
ABE ABE Lab 4, 5 Confirmation, Transformation due Thursday, 11/18
Read Ch 5.3 Protein Catalysts and do review questions
B.O. Catalysts for Better Health pg 151
Read ABE Lab 7 Overnight Cultures of Transformed E.coli; do prelab questions
Thursday, Nov 18
In class:
Go over ABE Lab 7 Overnight Cultures of Transformed E.coli
Ch 5.2 and 5.3 Protein Production and Protein Activity; correct review questions
Lab Activity: ABE Lab 7 Overnight Cultures of Transformed E.coli
Due today: ABE Lab 4 and 5
Homework:
Finish ABE Lab 6 and 7 Isolation of RFP by Column Chromatography – due on Tue 11/30
Read Ch 5.4 Studying Proteins and do review questions
11/08/202111/08/2021
M/W P1, P3, P5, P7
Thursday – Veteran's Day, No School
T/F P2, P4, P6, P7
Tuesday, Nov 9
In class:
Review ABE Lab 4 and 5 – Co Confirmation of Digest and Transformation
Lab Activity today – ABE Lab 6 Set up Overnight Culture of Transformed Cells; Screen for Transformed, red cells
Quiz on ABE Lab 4, 5 Confirmation, and Transformation
Due today- Ch 4 Packet
Homework:
ABE ABE Lab 4, 5 Ligation, Transformation due Thursday, 11/8
Read Ch 5.1 Protein structure and function and do review questions
B.O. Antibody Producing Companies
Read ABE Lab 6 Overnight Cultures of Transformed E.coli; do prelab questions
Wednesday, Nov 10
In class:
Go over ABE Lab 6 Overnight Culture
Ch 5.2 Protein Production and Protein Activity
Homework:
Read ABE Lab 7 Isolation of RFP by Column Chromatography
Read Ch 5.2 Protein Production
B.O. Couch Potatoes pg 146
11/01/202111/01/2021
T/Th P1, P3, P5, P7
W/F P2, P4, P6, P7
Tuesday, 11/2
In class:
Review ABE Lab 2 and 3 – Restriction Digest of pAra and pKan-R and Ligation
Lab Activity today – ABE Lab 4 Confirmation of Digest
Ch 4 Biotech Live – do Activities 4.1-4.3
ABE Labs 2 and 3 lab reports due on Thursday, 11/4
Quiz on ABE Lab 1 and 2
Homework:
Read ABE Lab 5 Transformation of E.coli with pAra-R
Complete ABE Lab 3 Ligation – Due on Tues, 11/9
Complete Ch 4 Biotech Live Activities 4.1-4.3
Thursday, Nov 4
In class:
Go over ABE Lab 4 Confirmation of Digest of pAra and pKan-R and Ligation
ABE Lab 5 Transformation
Complete Ch 4 Biotech Live Activities 4.4-4.6
Due today: ABE Labs 2 and 3 lab reports
Homework:
Read ABE Lab 6 Overnight Culture of Transformed E.coli
Finish ABE Labs 4 report – due Thurs 11/11
Complete Ch 4 Biotech Live Activities 4.4-4.6
Ch 4 packet due on Tue 11/9
10/25/202110/25/2021
M/Th P1, P3, P5, P7
W P1-P7 Banking Day
T/F P2, P4, P6, P7
Monday, Oct 25
In class:
Go over Gel Electrophoresis
Correct 4.3-4.4 review questions; Ch 4 Test on Friday, 10/29
Set up ABE Lab 2 Restriction Digest of pAra and pKan
Due today: Gel Electrophoresis and Restriction Enzyme Activity,
ABE Lab 1 Gel Electrophoresis – due on Thurs, 10/26
Homework:
Read ABE Lab 2 and 3
Do Biotech Live 4.1-4.6
Study for Ch 4 Test – Test on Fri, 10/29
Friday, Oct 29
In class:
Ch 4 Test
Go over ABE Lab 2 and set up ABE Lab 3 Ligation
Homework:
Read ABE Lab 4 Confirmation of Restriction Digest
Finish ABE Labs 2 and 3 report – due Thurs 11/5
10/18/202110/18/2021
M/Th P1, P3, P5, P7
W P1-P7 Banking Day
T/F P2, P4, P6, P7
Tuesday, Oct 19
In class:
Go over Math Problems and Ch 4.1 - 4.3 DNA Structure and Isolation of DNA
Quiz on Ch 4.1-4.3
Lab Activity: ABE Lab Gel Electrophoresis
Due: Strawberry DNA Isolation Lab
Homework:
Read Ch 4.4 Gel Electrophoresis and do review Questions
Strawberry DNA Lab – due on Tuesday
Thursday, Oct 21
In class:
Go over Ch 4.4 Gel Electrophoresis
Quiz on Gel Electrophoresis
Homework:
Do Biotech Online Chop and Go pg 124
Finish ABE Lab 1 Gel Electrophoresis – due on Tuesday 10/26
Read Ch 4 Summary and Lab Practices pgs 126-127
Ch 4 Test on Thursday, 10/28
10/11/202110/11/2021
M/Th P1, P3, P5, P7
W PSAT 8:30-12;30
T/F P2, P4, P6, P7
Tuesday, Oct 12
In class:
Go over Math Problems
Ch 4.1 DNA Structure
Lab Activity: Isolation of Strawberry DNA
Due today: Ch 3 Review questions and Math Problems
Homework:
Read Ch 4.2 Sources of DNA, do review questions
Do Biotech Online Know your Genome pg 112, Baldness Gene pg 118
Download Amgen Lab Manual
Strawberry DNA Lab – due on Tuesday
Thursday, Oct 14
In class:
Ch 4.2 Sources of DNA, correct review questions
Activity: Cast a Gel for Gel Electrophoresis; make 1x SB buffer and 1% agarose gel
Homework:
Read Ch 4.3 Isolating and Manipulating DNA
Do Biotech Online Recombinant Pharmaceuticals and Therapies, pgs 118-119
Read Lab 1 Amgen Lab Manual
Strawberry DNA Lab – due on Tuesday
10/04/202110/04/2021
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Tuesday, Oct 5
In class:
Correct Ch 3.6 review questions and Math Problems
Ch 3 Test on Thursday
Due today: Pipetting Lab and DNA Lab
Lab Activity: Biotech Live – Activity 3.1-3.4 pgs 95-97 (work on Chromebook)
Homework:
Study for Ch 3 Test – Test on Thursday
Work on Math problems/Find MW of compounds
Thursday, Oct 7
In class:
Ch 3 Test
Continue with Biotech Live – Activity 3.5-3.6 pgs 98-100
Go over Math/MW of compounds
Homework:
Read Ch 4.1 Studying DNA and do review questions
Read Lab 1 Amgen Lab Manual
Bring Strawberries for Tuesday
09/27/202109/27/2021
M/Th P1, P3, P5, P7
W P1-P7 (Banking Day)
T/F P2, P4, P6, P7
Tuesday, Sept 28
In class:
Correct all Ch 3.1 – 3.2 review questions and math problems
Go over Pipetting Lab part 1
Lab Activity – Pipetting Accuracy part 2
Homework:
Read Ch 3.5 and do review questions
Pipetting Accuracy Lab parts 1 and 2 – due on Tuesday, 10/5
Work on Math problems
Thursday, Sept 30
In class:
Go over Ch 3.5 review questions and math problems
Go over Pipetting Accuracy
Lab Activity – Micropippetting Exercise with colored dyes
Homework:
Read Ch 3.6 and do review questions
Read Summary and Lab Practices pgs 92-93
Pipetting Accuracy Lab parts 1 and 2 - due Tuesday, 10/5
Ch 3 Test – Tuesday, 10/5
Finish Math Problems
Date Assigned: 09/20/2021
Date Due: 09/20/2021
M/Th P1, P3, P5, P7
W P1-P7
T/F P2, P4, P6, P7
Tuesday, Sept 21
In class:
Correct all Ch 2.4 and 2.5review questions
Go over Bacteria Lab and Gram Stain
Ch 2 Test – today; Ch 2 packet due
Lab Activity – Gram Stain and use microscope to view bacteria
Homework:
Read Ch 3.1-3.2 pgs 71-82 and do review questions
Do B.O. pg 74
Finish Bacteria Lab and Gram Stain Lab – due on Tuesday, 9/28
Thursday, Sept 23
In class:
Go over Ch 3.1-3.2 review questions and math problems
Go over Gram Stain Lab
Lab Activity – Pippeting
Homework:
Read Cj 3.3-3.4 and do review questions
Do Math Problems
Grams Stain Lab – due on Monday, 9/28
Pipetting Lab due Tuesday, 9/29
09/13/202109/13/2021
M/Th P1, P3, P5, P7
W P1-P7 Banking Day
T/F P2, P4, P6, P7
Tuesday, Sept 14
In class:
Go over Organic Lab and complete Cheese Lab
Go over Microscope Lab
Correct Ch 2.1-2.3 review questions
Lab Activity – Bacteria Lab: collect bacteria, streak media plate, test against antibiotics
Homework:
Read Ch 2.4 New Biotechnology and do 2.4 review questions
Do B.O. pg 52 and 82
Finish Cheese Lab and Organic Lab - both are due on Thursday, 9/14
Microscope Lab – due on Tuesday 9/14
Ch 2 Test on Tuesday
Thursday, Sept 16
In class:
Due today: Cheese Lab and Organic Lab
Correct Ch 2.4 review questions
Go over Bacteria Lab
Lab Activity: Gram Staining
Homework:
Finish Bacteria Lab – due on Tuesday
Microscope Lab – due on Tuesday 9/14
Read Ch 2 Summary and Lab Practices pg 81-86
Ch 2 Test on Tuesday
09/06/202109/06/2021
Tuesday, Sept 7
In class:
Review Ch 1.3-1.6 and correct review answers
Discuss careers and Biotethics in Biotechnology- watch video on Biochemist
Make 2nd Observation of Cheese Lab – after 5 days, make cheeseball using cheesecloth
Lab Activity: Organic Lab
Homework:
Read Summary and Lab Practices on pgs 30-31 to review Ch 1Test
Study for Ch 1 Test – Test on Thursday, Sept 9
Cheese Lab is due on Tuesday, Sept. 14
Read Ch 2.1, do review question and Label parts of Microscope (worksheet) due on Thursday 9/9
Remember to copy review questions and answer in complete sentences on a separate sheet
Thursday, Sept 9
In class:
Ch 1 Test in class on Quizziz
Go over Ch 2.1 Review Questions, Organic Lab, and Microscope worksheet
Lab Activity: Organic Microscope and do cells worksheet on structures and function
Homework:
Read Ch 2.2-2.3, do review questions; remember to copy questions and answer in complete sentences
Complete cell structure and function worksheet
Finish Cheese Lab – due on Tues, 9/14
Finish Organic Lab – due on Thur, 9/16
08/30/202108/30/2021
Tuesday, August 31
In Class:
What is Scientific Method?
Correct Ch 1.3 review questions
Lab Activity: Cheese Lab
Bleach Lab due – send to intern to check for content/format/completion; see below
HW:
Read ED Ch. 1.4 and review questions pgs 19-23 ED
Extra Credit: make cheese at home; DIY on foodwishes.com
Thursday, Sept 2
In Class:
Cheese Lab - Make 1st observation, record in your notebook
Discuss Careers in Biotech
Correct Ch 1.4 review questions
HW:
Read ED Ch 1.5-1.6 and do review questions pgs 23-29 ED
Do B.O. Hot Jobs pg 26 and Code of Business Conduct pg 29 ED
Read Summary and Lab Practices pgs 30-31
Please share your lab report with your intern to get it checked before turning it in to me.
Biotech Interns:
Rosalia Park - rpar7075@stu.gusd.net A-C (Last names)
Iris Milanovich-Rooney - mil3828@stu.gusd.net D-H
Kalia Manayan - Kman4559@stu.gusd.net J-K
Claire Yang - cyan6089@stu.gusd.net L-M
Michelle Chung - mchu2590@stu.gusd.net P-S
Nathan Pang - npan1489@stu.gusd.net W-Y
08/23/202108/23/2021
Day 1 Tuesday, August 24
In class:
What is Biotech?
Go over How to maintain a Scientific Notebook in Biotech
Lab Activity: Bleach Lab pgs 19-21 TB
HW:
Read Ch 1.1-1.2 and do review questions Ch 1.1-1.2 in TB
Read Cheese Lab before Thursday and bring whole milk and buttermilk on Thursday
Day 2 Thursday, August 26
In class:
Make final observations for Bleach Lab, lab report due on Tues 8/31
Review How to Notebook
Lab Activity: Cheese Lab
HW:
Read Ch 1.3 pgs 14-18 and do review questions 1.3 TB
Do Biotech Online (B.O.) pg 18
Finish Bleach Lab report in Google Docs pgs 19-21 TB; lab report on Tuesday
08/18/202108/18/2021
Week 1 Semester 1 August 18-20, 2021 Assignments in Biotech
Day 1 Wednesday, August 18
In class:
Rules and Policies
Safety Rules
HW:
Sign up for Google Classroom, Quizizz
Study for Safety Test – Test on Thursday
Wear shoes to class – Always!
Day 2 Thursday, August 19
In class:
Safety Test
What is Biotechnology
HW:
Read Ch 1.1 Biotechnology ED (Ellyn Daugherty textbook)
Read Bleach Lab pgs 19-21 ED, Lab on Tuesday
Wear shoes please
06/07/202106/07/2021
Finals Week: We will meet on T/Th at 1:30 pm
Tuesday, June 8
In class:
Finish Career Exploration Project – Oral Presentations
Final Exam – Part 1
HW:
Study for Finals, Ch 6-9 – Finals Part 2 on Thursday
Thursday, June 10
In class:
Finals Part 2
HW:
Have a great summer!
05/31/202105/31/2021
Tuesday, June 1
In class:
Career Exploration Project – Oral Presentations
HW:
Study for Finals – Finals on Tuesday, June 8th and June 10th
Thursday, June 3
In class:
Continue with Oral Presentations on Career Exploration
HW:
Study for Finals – Finals on Tuesday, June 8th and June 10th
05/24/202105/24/2021
Tuesday, May 25
In class:
Resumes and Career Exploration Project – due today
Presentations on Career Exploration Project – begin today
HW:
Study for Finals – Finals on Tuesday, June 8th
Thursday, May 27
In class:
Continue with Oral Presentations on Career Exploration
HW:
Study for Finals – Finals on Tuesday, June 8th
05/17/202105/17/2021
Tuesday, May 18
In class:
Ch 13.1 and 13.2 DNA Synthesis – Groups 1 and 2 presentations
Work on Career Exploration Project
HW:
Read Ch 13.3 Polymerase Chain Reaction and do review questions
EP: PCR
Thursday, May 20
In class:
Ch 13.3 PCR – Group 3 and 4 presentations
HW:
Read Ch 13.4 Applications of PCR and do review questions
B.O. – CSI – your room pg 383
EP: Karyotyping/DNA Fingerprinting
05/10/202105/10/2021
Tuesday, May 11
In class:
Review Ch 12 Medical Technologies
Ch 13.1 DNA Synthesis – Group 10 presentation
HW:
Read 13 DNA Synthesis pg 365-371 and do review question
B.O. Telomeres pg 369
B.O. Snip here, snip there pg 371
Ch 12 Test on Thursday
Thursday, May 13
In class:
Ch 12 Medical Technologies Test – today
Ch 13.2 DNA Synthesis Products – Group 10 presentation
HW:
Read Ch 13.2 DNA Synthesis Products and do review questions
EP: PCR
05/03/202105/03/2021
Tuesday, May 4
In class:
Stem Cell Module Day 3 – instructions provided by Dr. Karol Lu from PCC CIRM Program
HW:
Complete Stem Cell Assignment from Dr. Lu
Read 12 Summary and Lab Practices pg 360-361
Thursday, May 6
In class:
Stem Cell Module Day 4 – instructions provided by Dr. Karol Lu from PCC CIRM Program
HW:
Complete Stem Cell Assignment from Dr. Lu
Do Biotech Live Ch 12 Activities 12.1-12.3
Study for Ch 12 Test – Ch 12 Test on Tuesday
04/26/202104/26/2021
Tuesday, April 27
In class:
Stem Cell Module Begins today, Day 1 – instruction provided by Dr. Karol Lu from PCC CIRM Program
HW:
Complete Stem Cell Assignment from Dr. Lu
Read 12.4 and do review questions 12.4
Do B.O. pg 355 Eating Vegetables
Thursday, April 29
In class:
Stem Cell Module Day 2 – instruction provided by Dr. Karol Lu from PCC CIRM Program
HW:
Complete Stem Cell Assignment from Dr. Lu
Read Ch 12.5 Recent Advances in Medical Biology and do review questions 12.5
Do B.O. pg 357 Medicine for you
04/19/202104/19/2021
Tuesday, April 20
In class:
Review Ch 9 Taking Product to Market
Ch 9 Test
Ch 12.1- Drug Discoveries, Group 9 presentation
HW:
Read 12.1-12.2 Drug Discoveries and Creating Pharmaceuticals
Do review questions 12.1-12.2
Do B.O. pg 344 New Medications
Do B.O. pg 345 Penicillin
Edpuzzle: Making Vaccines due on Thursday, April 22 @ 8 am
Quiz on Thursday
Thursday, April 22
In class:
Review Ch 12.1 Drug Discoveries
Ch 12.2 Creating Pharmaceuticals - Group 9 Presentation
HW:
Read Ch 12.3-12.4 Creating Pharmaceuticals Through Peptide and DNA Synthesis
Do Review Questions
Do B.O. pg 354 Getting Sick
Do B.O. pg 355 Eat your Vegetables
Edpuzzle: mRNA Vaccines due Fri 4/23
04/12/202104/12/2021
Tuesday, April 13
In class:
Due today: Amgen Lab 7 Isolation of GFP protein using Column Chromatography
Due Thursday: Labster HPLC Lab Report
Ch 9.4 Product Quality Control, correct review questions - Group presentation
HW:
Read Ch 9.5 Marketing and Sales, do review questions
Do B.O. pg 268 Got Gas
Do B.O. pg 270 Products on Pipeline
Quiz on Thursday, 4/15
Edpuzzle: Quality Control due on Thursday, April 15 @ 8 am
Thursday, April 15
In class:
Due today: Labster HPLC Lab Report
Review Ch 9.4 Product Quality Control
Group Presentation - Ch 9.5 Marketing and Sales
HW:
Do B.O. pg 273
Read Summary pg 274 and Lab Practices pg 275
Biotech Live: pg 276-277
Ch 9 Test on Thursday, 4/22
04/05/202104/05/2021
Tuesday, April 6
In class:
Due today: LabXchange Activity – Column Chromatography Lab Report
Go over Amgen Lab 6/Lab 7 O/N culture and Isolation of GFP protein using Column Chromatography
Ch 9.2 Using Chromatography to Separate Molecules, correct review questions - Group presentation
Lab Activity: Amgen Lab 7 Purification Unit Column Chromatography – Lab Report due Tues 4/13
HW:
Read Ch 9.3 Column Chromatography, FPLC, HPLC, do review questions
Edpuzzle: HPLC due on Thursday, April 8 @ 8 am
Thursday, April 8
In class:
Review Amgen Lab 7 – Isolation of GFP using Column Chromatography
Review Ch 9.2 Chromatography to separate molecules
Group Presentation - Ch 9.3 more on Column Chromatography
HW:
Read Ch 9.4 Product Quality Control, do review questions
Biotech Online 9.4 pg 279
Amgen Lab 7 Purification of GFP by Column Chromatography Lab Report – due Tues, April 13
03/29/202103/29/2021
Tuesday, Mar 29
In class:
Due today: Labster Activity – Fermentation Lab Report
Go over Amgen Lab 6 O/N culture of pGLO Transformed Cells
Go over Ch 8.4 Fermentation and Ch 8.5 Retrieving Plasmids
Ch 8 Test today
Ch 8 packet - due today
HW:
Read Amgen Lab 7 Purification Unit Column Chromatography
Read Ch 9.1 Taking Product to Market and do review questions
Labster Activity: Column Chromatography, Lab report due on Tues, 4/6
Thursday, April 1
In class:
Group Presentation - Ch 9.1 Taking Product to Market and correct review questions
Lab Activity: Amgen Lab 7 Purification Unit Column Chromatography
HW:
Read Ch 9.2 Chromatography and do review questions
Labster Activity: Column Chromatography, Lab report due on Tues, 4/6
03/22/202103/22/2021
Tuesday, Mar 23
In class:
Create Spreadsheet for LAMC Student Codes
Continue Design Project Oral presentations
Go over Scaling up and Amgen Lab 6 Overnight Culture
Ch 8.4 Fermentation – Group presentation
HW:
Labster Activity: Fermentation – Lab report due on Tuesday 3/30
Read Ch 8.5 Retrieving Plasmids; do review questions
Ch 8 Test on Tuesday 3/30
Ch 8.1-8.3 Quiz on Thursday 3/25
Thursday, Mar 25
In class:
Ch 8.1-8.3 Quiz Genetic Engineering and Transformation
Go over Ch 8.4 Fermentation
Ch 8.5 Retrieving Plasmids - Group Presentation
HW:
Read Ch 8 Summary and Lab Practices pgs 246-248
Do Biotech Live: Activity 8.1 Lambda phage, Activity 8.2 Gene Bank/NCBI, Activity 8.3 GMP due 3/30
Finish Labster Activity: Fermentation – Lab report due on Tuesday 3/30
Ch 8 Test on Tuesday 3/30
03/08/202103/08/2021
Tuesday, Mar 9
In class:
Design Project Oral presentations begin today - Synthetic Biology Design Project with Tinker Cell
Go over Amgen Lab 4 and 5 Confirmation of Restriction Digest and Transformation
Quiz on Amgen Lab 4 and 5
Ch 8.3 After Transformation – presentation by Talin's Group
Lab Activity: Set up O/N culture
HW:
Finish Amgen Labs 4 and 5 Lab Reports – due on Thurs 3/11
Read Amgen Lab 6 Overnight Culture
Read Ch 8.3 After Transformation; do review questions
Thursday, Mar 11
In class:
More Oral Presentations -Synthetic Biology Design Project with Tinker Cell
Due today: Amgen Labs 4 and 5
Go over Amgen Lab 6 Overnight Culture
Go over Ch 8.3 After Transformation
HW:
Read Ch 8.4 Fermentation, Manufacturing, and Production; do review qs
Read Amgen Lab 6 O/N Culture of Transformed HB101
Do prelab notes lab 6
03/01/202103/01/2021
Tuesday, Mar 2
In class:
Design Project due on Thursday, oral presentations on Tuesday 3/9
Go over Ch 8.1-8.2 Genetic Engineering and Transformation
Go over Amgen Lab 2 Restriction Digest, pDRK and pGRN and Amgen Lab 3 Ligation
Lab Activity: Amgen Lab 5 Transformation
HW:
Do Biotech Online pg 230 Real Cut Ups and Biotech Online RFLP pg 231
Read Amgen Lab 4 Confirmation of Restriction Digest
Do prelab notes 2-5
Thursday, Mar 4
In class:
Due today: Synthetic Biology Design Project with Tinker Cell
Go over Amgen Labs 2-5
Go over Amgen Lab 5 Transformation – Screen for green colonies
Lab Activity: Amgen Lab 4 – Confirmation Digest of pDRK and pGRN
HW:
Complete Amgen Labs 2-3 lab report – due Tues 3/9
Amgen Labs 4 & 5 due on Thurs 3/11
Synthetic Biology Oral Presentation – due Tues 3/9
02/22/202102/22/2021
Tuesday, Feb 23
In class:
Questions on your Design Project on Synthetic Biology – Design Project due next week
Ch 8.1 Genetic Engineering – ppt presentation by Grp 5
Due on Thursday: pH Lab Activity: pHun at Home – due 2.25
HW:
Read Ch 8.1 Genetic Engineering; do review questions 8.1 – copy questions, answer in complete sentences
Do Biotech Online pg 226 Genetic Engineering
Download Amgen Lab Green Manual
Read Amgen Lab 2 Restriction Digest pGRN and pDRK and Lab 3 Ligation
Thursday, Feb 25
In class:
Due today: pH Lab Activity: pHun at Home
Correct Ch 8.1 review questions
Ch 8.2 Transforming Cells – ppt presentation by Grp 5
Lab Activity: Amgen Lab 2 – Restriction Digest of pGRN and pDRK
Lab Activity: Amgen Lab 3 Ligation - to construct a recombinant DNA, rpGLO
HW:
Read Ch 8.2 Transformation and do review questions 8.2
Do Biotech Online pg 228 BACS vs YACS
Read Amgen Lab 5 Transformation of E.coli using rpGLO
EP: Watch Restriction Enzymes – DNA Learning Channel
02/15/202102/15/2021
Tuesday, Feb 16
In class:
Questions on your Design Project on Synthetic Biology – Design Project due in 2 weeks
Ch 7.3 Buffers – ppt presentation by Grp 4; correct 7.1 review questions
Quiz on pH and Buffers
HW:
Review Chapter 7; Ch 7 Test on Thursday, 2/18
Read Ch 7 Summary and Lab Practices, pg 214-216
Finish Labster Acids and Bases Lab report due on Thursday, 2/18
Thursday, Feb 18
In class:
Ch 7 Test
Due today: Labster Acids and Bases Lab report due on Thursday, 2/18
Do Activity 7.1 Biotech Live pg 216
HW:
Do Biotech Live Activity 7.2 Phun at home – pg 216
Do Biotech Live Activity 7.3 Buffer – pg 217
02/08/202102/08/2021
Tuesday, Feb 9
In class:
Questions on your Design Project on Synthetic Biology – Design Project due in 2 weeks
Due today: Lab 6d Potential Medicine Lab report
Due today: Labster Activity: Spectrophotometers Lab Report
Ch 7.5 Spectrophotometer – ppt presentation by Grp 3; correct 7.1 review questions
Quiz on Ch 7.1, 7.4, 7.5 Use of Spectrophotometer to find concentration
HW:
Read Ch 7.2 Introduction to pH meters and do review questions 7.2
Do Biotech Online (BO) pH meters pg 203
Edpuzzle: pH meters
Thursday, Feb 11
In class:
Review Spectrophotometers
Ch 7.2 pH meters – Group 4 to present
Quiz on pH meters
HW:
Labster Activity: Acids and Bases and pH meters
Read Ch 7.3 Buffers and do review questions 7.3
pH meter Lab report due on Thursday, 2/18
NEXT WEEK: Ch 7 Test – Thursday, 2/18
02/01/202102/01/2021
Tuesday, Feb 2
In class:
Questions on your Design Project on Synthetic Biology? – Please ask
Go over Lab 6d Potential Medicine –Lab report due on Tuesday, Feb 9
Ch 7.1 Spectrophotometer – ppt presentation by Grp 3; correct 7.1 review questions
Quiz on Ch 7.1 Spectrophotometer
HW:
Read Ch 7.4 recombinant protein and do review questions 7.4 pgs 209-211
Do Biotech Online (BO) FTIR pg 200
Labster Activity: Spectrophotometers - Lab Report due on Tuesday, 2/9
Thursday, Feb 4
In class:
Review Spectrophotometers
Ch 7.4 Spec to measure protein concentration –Group 3 to present
Quiz on Ch 7.4 Spectrophotometers
HW:
Read Ch 7.5 Spectrophotometers to measure DNA Conc; do review qs 7.5, pgs 211-213
Finish Lab report Lab 6d Potential Medicine – due Tuesday, 2/9
Finish Lab report Labster Spectrophotometer Activity - due Tuesday, 2/9
NEXT WEEK: we will cover Ch 7.2 and 7.3
01/25/202101/25/2021
Tuesday, Jan 26
In class:
Questions on your Design Project on Synthetic Biology? – Please ask
Review Ch 6.3-6.4 ELISA Lab and Western Blot
Quiz on Ch 6.3-6.5
Ch 6.5 and 6.6 Potential Medicine and Recombinant protein – ppt presentation by Grp 2;
correct 6.5 review questions
Lab Activity: Potential Medicine – test your plant extract against E.coli
ELISA Anti-heparin and Rapid ELISA Hemosure (IFOBT) – due on Thursday, Jan 28
HW:
Finish Lab Activity to look for Potential Medicine against E.coli
Read Ch 6.6 recombinant protein and do review questions 6.6
Do Biotech Online (BO) pg 183
Read Ch 6 summary pg 184 and Lab practices pg 185 – to review Ch 6
Ch 6 Test – on Thursday
Thursday, Jan 28
In class:
Due today: ELISA Lab Report – Anti-Heparin ELISA and Rapid ELISA
Review Ch 6.6 and correct Ch 6.6 review qs
Ch 6 Test
Ch 6 packet due – should contain Ch 6.1-6.6 review questions/answers
HW:
Read Ch 7.1 Spectrophotometers; do review qs 7.1
Do Virtual Spectrophotometer Lab
01/18/202101/18/2021
Tuesday, Jan 19
In class:
Questions on your Design Project on Synthetic Biology? – Please ask
Review Ch 6.2 Assays
Ch 6.3 ELISA – ppt presentation by Grp 1; correct 6.3 review questions
Lab Activity: Demo ELISA
Lab Activity: Rapid ELISA Hemosure (IFOBT) you will do this assay at home (materials are in lab bag)
HW:
Do Biotech Online (BO) pg 173
Finish Labster ELISA Lab report – due Thurs
Read Ch 6.4 Western Blot and do review questions 6.4
Quiz on Thursday (Ch 6.3 and 6.4)
EP: Western Blot
Continue to work on building your biological system
Thursday, Jan 21
In class:
Due today: ELISA Lab Report
Update on your Design Project – start putting together your parts to build your device
Review Ch 6.3 ELISA
Ch 6.4 Western Blot presentation by Grp 2 and correct 6.4 review questions
Quiz on 6.3 and 6.4
Lab Activity: Looking for Potential Medicine – you will need to use fresh herbs, mortar and pestle
HW:
Read Ch 6.5 New Products
Do review questions 6.5 and BO pg 179
Complete Lab report on Rapid ELISA Hemosure (IFOBT) – Lab report due on Thursday
Continue to work on building your biological system
01/11/202101/11/2021
Tuesday, Jan 12
In class:
More on Synthetic Biology: Today, we will learn how to use the Registry of Standard Biological Parts to look for parts to build your device for your biological system. Work in breakout rooms.
Ch 6.1 Identifying Potential Products – ppt presentation by Grp 1
HW:
Read Ch 6.2 Assays
Do review questions 6.2; quiz on Thursday (6.1 and 6.2)
Continue to work on building your biological system
Thursday, Jan 14
In class:
Update on your Design Project –review Registry of Standard Biological Parts build your device
Ch 6. Identifying Potential Products – ppt presentation by Grp 1
Quiz on Ch 6.1-6.2
Edpuzzle: ELISA Assays
HW:
Read Ch 6.3 ELISA
Do review questions 6.3
Labster Activity - ELISA
Continue to work on building your biological system
01/04/202101/04/2021
Day 1 Thursday, Jan 7
In class:
More on Synthetic Biology: Go over Tinker Cell – a CAD program (Computer-Aided Design) to design your biological system
Assign chapters for group presentations
HW:
Read Ch 6.1 Assays
Do review questions 16.1
EP Basics of Tinker Cell - Watch the Tinker Cell Tutorial and practice with it
12/07/202012/07/2020
Tuesday, Dec 8 Agenda
In class:
What is Synthetic Biology?
Explore Biobuilder.org
Synthetic Biology Design Group Project – breakout rooms to work on choosing a global problem to solve with your group and you will present your proposal during finals week.
HW:
Continue to work on choosing a global problem – explore Biobuilder.org
Group Presentations on Tuesday and Thursday, Dec 15 and 17 in class – create a power point presentation on your proposal
Thursday, Dec 10 Agenda
In class:
Learn more about Synthetic Biology
Work on Design Project proposal
HW:
Continue Group Project: work on your proposal
Group Presentations on Tuesday and Thursday, Dec 15 and 17 in class – create a power point presentation on your proposal
11/30/202011/30/2020
Tuesday, Nov 30 Agenda
In class:
Due today: Ch 5 packet, PAGE Lab report
NCBI/Bioinformatics Activity – breakout rooms to work on genetic disorder
HW:
Continue to work on genetic disorder using NCBI
Group Presentations on Thursday, Dec 3 in class – create a power point presentation on your genetic disorder
Thursday, Dec 3 Agenda
In class:
Group presentations on your genetic disorder and bioinformatics
HW:
Group Project: Synthetic Biology
Pick a global problem that your group will solve
Groups will present your global problem during finals week
11/16/202011/16/2020
Tuesday, Nov 17 Agenda
In class:
Go over Ch 5.4 Analyzing Proteins and RFP Isolation by Column Chromatograph
Correct Ch 5.4 review questions
Edpuzzle – PAGE, Polyacrylamide Gel Electrophoresis to analyze RFP
https://www.youtube.com/watch?v=i_6y6Z5UvwE
HW:
Study for Ch 5 Test – Test on Thursday
Read Summary pg 158-159 to get ready for the test
Do B.O. pg 156
Amgen Lab 7 Lab Report due on Thursday 11/19
Catalase Lab Report due on Thursday 11/19, include 3 data tables and 3 graphs
Thursday, Nov 19 Agenda
In class:
Ch 5 Test
Go over PAGE results
HW:
Do Biotech Live 5.1 Protein of Interest,
5.2 AA Sequence of Insulin
5.3 Prions
PAGE Lab Report – due on Tuesday, Dec 1
11/09/202011/09/2020
Tuesday, Nov 10 Agenda
In class:
Go over Ch 5.3 Enzymes and Catalase Lab
Correct Ch 5.3 review questions
Lab report due today – Protein Synthesis by Labster
HW:
Lab Activity Amgen Lab 7 – Isolation of protein RFP from the red colonies by Column Chromatography
Read Ch 5.4 The production of proteins, do review questions 1-4
Amgen Lab 7 due on 11/17
Continue Catalase lab at home – this time investigate – Investigate the effect of temp and H202 concentration - Catalase lab due on Thursday 11/19
Thursday, Nov 12 Agenda
In class:
Review Ch 5.4 Studying Proteins, correct review questions
Go over Amgen Lab 7 Isolation of RFP protein
HW:
Catalase lab due on Thursday 11/19
Amgen Lab 7 due on 11/17
Study for Ch 5 Test – Test on Thursday 11/19
Read about PAGE – Polyacrylamide Gel Electrophoresis
11/02/202011/02/2020
Tuesday, Nov 3 Agenda
In class:
Ch 5.1 Structure and Function of Proteins
Correct Ch 5.1 review questions
Lab Activity – Antibody-Antigen test using rapid ELISA Test; this is in your bag of materials
Amgen Lab 6 - Set up Overnight Culture of red colonies to produce RFP (in classroom)
HW:
Read Amgen Lab 6 - Overnight Culture of red colonies to produce RFP (in classroom)
Read Ch 5.2 The production of proteins, do review questions 1-4
Labster Activity – Protein Synthesis; you will need to do a lab report on this, due on Tuesday, 11/10
Thursday, Nov 5 Agenda
In class:
Go over Amgen Lab 6 Overnight Culture; get ready for Amgen Lab 7 Isolation of RFP protein
Correct Ch 5.2 Production of proteins
HW:
Read Ch 5.3 Enzymes: Protein Catalysts, do review questions 1-4
Do B.O. Enzymes: Catalysts for Better Health pg 151
Read Amgen Lab 7 Isolation of RFP protein
Finish Protein Synthesis Lab Report due 11/9, and Antibody-Antigen Rapid ELISA lab due 11/9
10/26/202010/26/2020
Tuesday, Oct 27 Agenda
In class:
Go over Amgen Labs 4 and 5 Confirmation of Digest and Bacterial Transformation
Quiz on Amgen Labs 4 and 5
Due today: Amgen Lab 4 Confirmation of Digest and Lab 5 Transformation lab reports
HW:
Review Ch 4 vocab words on Quizlet
Study for Ch 4 Test – Test on Thursday, Oct 29
Study Amgen Labs 1-5 – Test on Thursday, Oct 29
Thursday, Oct 29 Agenda
In class:
Ch 4 Test and Amgen Labs 1-5 Test
Ch 5 Proteins Structure and Function
HW:
Read Ch 5.1 Proteins Structure and Function, do review questions 1-4
B.O. pg Antibody-Producing Companies pg 141
Khan Academy RNA and Protein Synthesis – log into your Khan Academy account
Read Amgen Lab 6 Growing Overnight Culture
10/19/202010/19/2020
Tuesday, Oct 19 Agenda
In class:
Go over Amgen Lab 3 Ligation and Lab 4 Confirmation of Digest
Quiz on Amgen Labs 1-4
Today: Amgen Lab 5 Transformation –in class demo
Due today: Amgen Lab 1 Gel Electrophoresis lab report
HW:
Read Amgen Lab 5 Transformation; do prelab notes
Amgen Labs 2 and 3 Lab Reports due on Thursday, 10/22
EP – Intro to Transformation and Transformation Lab Biorad
Thursday, Oct 21 Agenda
In class:
Review Amgen Lab 5 Transformation
Activity: Screen for red colonies
Quiz on Amgen Lab 5 Transformation
HW:
Amgen Labs 4 and 5 Lab Reports due 10/27 Tuesday
Study for Ch 4 Test – Test on Tuesday
Amgen Labs 1-5 Test on Thursday
10/12/202010/12/2020
Tuesday, Oct 13 Agenda
In class:
Correct Ch 4.3-4.4 review questions
Go over Amgen Lab 1 Gel Electrophoresis
Quiz on Ch 4.1-4.2 DNA
Amgen Lab 2 Restriction Digest –in class demo
HW:
Read Amgen Lab 2 Restriction Digest and Lab 3 Ligation, do Lab 2 and 3 prelab questions
Do B.O. pg 112, 116
Amgen Lab 1 Gel Electrophoresis Lab Report due on Tuesday, 10/20
Thursday, Oct 15 Agenda
In class:
Review Amgen Lab 2 Restriction Digest and Lab 3 Ligation
Amgen Lab 3 Ligation – in class demo
Amgen Lab 4 Confirmation of Digest – in class demo
HW:
Read Amgen Lab 4 Confirmation of Digest and Lab 5 Transformation do prelab questions
Do B.O. pg 118
Amgen Labs 2 and 3 Lab Reports due 10/22 Thursday
10/05/202010/05/2020
Tuesday, Oct 5 Agenda
In class:
Go over Math Problems and correct Ch 3.5 review questions
Ch 3 Test; Upload Ch 3 packet by Wednesday 8 am
Ch 4.1-4.2 DNA
Discuss Manipulating DNA, Amgen Recombinant DNA Curriculum
HW:
Read Ch 4.2-4.3 ED and do review questions
Download Amgen Red Lab Manual
Read Amgen Lab 1 Gel Electrophoresis, do Lab 1 prelab questions
Do DNA Isolation Lab at home – lab report due Tuesday, 10/13; I will demo this in class today.
Thursday, Oct 9 Agenda
In class:
Go over Ch 4.1-4.3 review questions
Go over Gel Electrophoresis
In class demo: Gel Electrophoresis
HW:
Review Ch 4.4 and do review questions
Watch Gel Electrophoresis on DNALC
Run Gel: https://dnalc.cshl.edu/resources/dnalc-live/watch/20200512-dnalc-shorts-gel-electrophoresis-17190
Read Amgen Lab 2 Restriction Digest; do prelab questions
Amgen Lab 1 Lab report due Tuesday, 11/13
09/21/202009/21/2020
Tuesday, Sept 22 Agenda
In class:
Review and correct Ch2.3 – 2.4 assignments; New Biotechnology
Review Organic Lab, Bacterial Isolation Lab, and pH Lab
Quiz on Organic molecules
Due today: Organic Lab
HW:
Study for Ch 2 Test; Test on Thursday on Quizziz
Read Ch 3.1-3.2 ED and do review questions 3.1-3.2
Do B.O. pg 74 and 78 ED
Labster Activity: Pipetting – Lab report due on 9/15
Thursday, Sept 24 Agenda
In class:
Review Chapter 2
Ch 2 Test
Review pipetting by DNA Learning Channel (DNALC)
Do Math problems
HW:
Read Ch 3.3-3.4 ED and do review questions 3.3-3.4
Work on Math Problems
Edpuzzle on MSDS (SDS) – watch, take notes and be prepared for a quiz
09/21/202009/21/2020
Tuesday, Sept 22 Agenda
In class:
Review and correct Ch2.3 – 2.4 assignments; New Biotechnology
Review Organic Lab, Bacterial Isolation Lab, and pH Lab
Quiz on Organic molecules
Due today: Organic Lab
HW:
Study for Ch 2 Test; Test on Thursday on Quizziz
Read Ch 3.1-3.2 ED and do review questions 3.1-3.2
Do B.O. pg 74 and 78 ED
Labster Activity: Pipetting – Lab report due on 9/15
Thursday, Sept 24 Agenda
In class:
Review Chapter 2
Ch 2 Test
Review pipetting by DNA Learning Channel (DNALC)
Do Math problems
HW:
Read Ch 3.3-3.4 ED and do review questions 3.3-3.4
Work on Math Problems
Edpuzzle on MSDS (SDS) – watch, take notes and be prepared for a quiz
09/14/202009/14/2020
Day 1 Sept 14
In Class
Ch 2 Macromolecules
Lab Activity: Organic Lab
Bacterial Isolation – learn how to isolate bacteria
Homework:
Read Ch 2.3 ED
Do review questions 2.3
Do B.O pgs 45 and 52
Labster Activity: Bacterial Isolation; you will need to do
Do at home: swab an area and inoculate your bacterial plate, incubate in a warm area
Day 2 Sept 16
In Class
Ch 2 Cells
Go over Organic Lab
pH Lab - demo
Homework:
Read Ch 2.4 ED
Do review questions 2.4
Do B.O pg 62
Read pg 69 answer questions #1,2
Do at home: pH Lab; round up some household liquids, use your spot plate and pH paper to conduct the lab 2 Test on Thursday
Organic Lab – due on Tuesday, 9/22
09/08/202009/08/2020
Tuesday, Sept 8
In class:
Review Ch 1.3-1.5 and correct review answers
Discuss careers in Biotechnology- watch video on Biochemist
Discuss Bioethics - watch Edpuzzle Ted Talk on Bioengineering
Bleach Lab due today
Homework:
Read Ch 1.6 and do review questions
Read Summary and Lab Practices on pgs 30-31 to review Ch 1Test
Study for Ch 1 Test – Test on Thursday, Sept 10
Cheese Lab is due on Thursday, Sept 10
Read Ch 2.1, do review question and Label parts of Microscope (worksheet in GC)
Thursday, Sept 10
Review and correct Ch 1.6 answers
Ch 1 Test in class on Quizziz
Go over Ch 2.1 Microscope, Cells: structures and function
In class: do Virtual Lab Tour of a Microscope
Homework:
Read Ch 2.2, do review questions; remember to copy questions and answer in complete sentences
Complete cell structure and function worksheet
Labster Lab Activity: Microscope Lab – lab report due next Thursday
Labster Lab Activity: Cell Structure – lab report due next Thursday
08/31/202008/31/2020
Tuesday
Read ED Ch. 1.3 pages 14-18 ED
Do review questions pg 19
Do B.O. pg 18
Bleach Lab due 9/3
Cheese Lab due 9/8 DIY on foodwishes.com
Thursday
Read ED Ch. 1.4 - 1.5 pages 19-23 ED
Do B.O. pg 26,29 ED
Read Summary and Lab Practices pg 30-31
Due today: Bleach Lab
Due on 9/8 Cheese Lab
08/24/2020 08/24/2020
Week 2 August 24-28, 2020 Assignments
Tuesday: Check Google Classroom for due date
Wednesday or Thursday ???? Check Google Classroom for due date
***Thursday is Back to School Night