T/Ths @ 2:50 pm

M     -   P1-P7 (Banking Day)

T/Th - P1, P3, P5, P7

W/F - P2, P4, P6, P7

Day 1 Tuesday, Aug 27

In class:

What is Biotech?

Go over How to maintain a Scientific Notebook in Biotech

Lab Activity: Bleach Lab pgs 19-21 TB

HW: 

Read Ch 1.1-1.2 and do review questions Ch 1.1-1.2 in ED - write questions and answers on paper with black ink

Read Cheese Lab before Thursday and bring whole milk and buttermilk on Thursday

Do Biotech Online, B.O., pg 13 Glo Fish

Day 2 Thursday, Aug 29

In class:

Complete and make final observations for Bleach Lab, lab report due on 9/7

Review How to Notebook and how to write an effective conclusion

Lab Activity: Cheese Lab 

HW: 

Read Ch 1.3 pgs 14-18 and do review questions 1.3 ED

Do Biotech Online (B.O.) pg 18 Genentech

Finish Bleach Lab report in Google Docs pgs 19-21 ED; lab report due on Th 9/7

*** Class only meets on Tuesdays and Thursdays

Day 1 Wednesday, August 21

In class:

Rules and Policies

Safety Rules

HW:

Sign up for Google Classroom, Quizizz, Edpuzzle

Study for Safety Test – Test on Thursday

Wear shoes to class – Always!

Day 2 Thursday, August 22

In class:

Safety Test

What is Biotechnology

HW:

Read Ch 1.1 Biotechnology ED (Ellyn Daugherty textbook)

Read Bleach Lab pgs 19-21 ED, Lab on Tuesday

Wear shoes, = please!

M     - Labor Day Holiday

T/Th - P1, P3, P5, P7

W/F - P2, P4, P6, P7

Tuesday, Sept 2

In Class:

Review Scientific Method

Correct Ch 1.2-1.3 review questions

Lab Activity: Cheese Lab, make first observation, record in your notebook

Biotech Live: Activity 1 What is Biotechnology? pgs 32-33

Activity 2 Business Side pgs 33-34

HW:

Read ED Ch. 1.4 and review questions pgs 19-23 ED

Do B.O. Hot Jobs pg 26 

Extra Credit: make cheese at home; DIY on foodwishes.com

Bleach Lab due Thurs 9/4; Did you send to intern to check for content/format?

Thursday, Sept 4

In Class:

Cheese Lab – Make 2nd observation, record in your notebook🡪 ready for cheese cloth?

Discuss Careers in Biotech

Do Biotech Live Activity 1.4 How Biotech Industry Improving Human Life? pg 35

Correct Ch 1.4 review questions

Bleach Lab due today– Did you send to intern to check for content/format?

HW:

Read ED Ch 1.5-1.6 and do review questions pgs 23-29 ED

Do B.O. Code of Business Conduct pg 29 ED

Read Summary and Lab Practices pgs 30-31

Ch 1 Test – Thurs 9/12

M/W – P1, P3, P5, P7

T/Th – P2, P4, P6, P7

Friday – Minimum Day

Tuesday, Sept 10

In class:

Review Ch 1.3-1.6 and correct review answers

Discuss careers and Biotethics in Biotechnology- watch video on Biochemist

Check cheeseball – make final observation: color, texture, smell

Go over Microscope Lab – due on Thurs, 9/12

Lab Activity: Organic Lab – lab report due on Thurs, 9/19

Homework:

Read Summary and Lab Practices on pgs 30-31 to review for Ch 1 Test

Study for Ch 1 Test – Test on Thurs, 9/12 (BRING CHROMEBOOK, charged)

Cheese Lab is due on Tues, 9/17

Read Ch 2.1, do review question 

Remember to copy review questions and answer in complete sentences on a separate sheet

Thursday, Sept 12 (BTSN)

In class:

Ch 1 Test in class on Quizziz

Go over Ch 2.1 Review Questions, Organic Lab, and Microscope worksheet

Lab Activity: Organic Lab on Tuesday

Homework:

Read Ch 2.2-2.3, do review questions; remember to copy questions and answer in complete sentences

Complete cell structure and function worksheet

Finish Cheese Lab - due on Tues, 9/17

Read Organic Lab – lab on on Tues, 9/17

M P1-P7 Banking Day

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Tuesday, Sept 17

In class:

Go over Organic Lab 

Correct Ch 2.1-2.3 review questions

Due today: Cheese Lab, Microscope Lab (was due on Friday)

Lab Activity – Bacteria Lab: collect bacteria, streak media plate, test against antibiotics

Homework:

Read Ch 2.4 New Biotechnology and do 2.4 review questions

Do B.O. pgs 43 Kimchi, 46 Right Tool, 52 Molecular Models, and 82 Biotech Products

Finish Organic Lab -  both are due on Thursday, 9/19

Ch 2 Test on Thursday, 9/26

Thursday, Sept 19

In class:

Due today: Organic Lab

Correct Ch 2.4 review questions

Go over Bacteria Lab and Gram Stain

Lab Activity: Continue Bacteria Lab; Gram Staining of bacteria

Homework:

Finish Bacteria Lab Report – due on Thursday, 9/26

Do Biotech Live Activity 2.1-2.3 pg 86

Read Ch 2 Summary and Lab Practices pg 81-86

Ch 2 Test on Thursday, 9/26

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Tuesday, Sept 24

In class:

Review Ch 2 The Raw Materials

Go over Bacteria Lab and Gram Stain

Lab Activity – Restreak bacteria with antibiotics; Grams Stain

Do SkillsUSA Certification Module

Homework:

Ch 2 Test on Thursday and Ch 2 packet due on Thursday

Read Ch 3.1-3.2 pgs 71-82 and do review questions

Do B.O. pg 74 on Metrics Conversion

Finish Bacteria Lab with antibiotics– due on Tuesday, 10/1

Gram Stain Lab – due on Thursday, 10/3

Thursday, Sept 26

In class:

Ch 2 Test and Ch 2 packet due today

Go over Ch 3.1-3.2 review questions and math problems

Go over Gram Stain Lab

Lab Activity – Pipetting Accuracy

Homework:

Read Ch 3.3-3.4 and do review questions

Do Math Problems                                                                                          

Bacteria Lab with antibiotics – due on Tuesday, 10/1

Gram Stain Lab – due on Thursday, 10/3

Pipetting Lab due Thursday, 10/3

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Tuesday, Oct 1

In class:

Correct all Ch 3.1 – 3.3 review questions and math problems

Go over Math Problems

Lab Activity – Pipetting Accuracy part 1 and 2

Due today: Bacteria Lab with antibiotics; Gram Stain Lab – due on Thurs, 10/3

Homework:

Read Ch 3.4 - 3.5 and do review questions

Do B.O. 150 Meter Homer pg 74, Positive Displacement pg 79,

Pipetting Accuracy Lab parts 1 and 2 – due on Tues, 10/8

Work on Math problems

Thursday, Oct 3

In class:

Go over Ch 3.5 review questions and math problems

Go over Pipetting Accuracy

Lab Activity – Micropippetting Exercise with colored dyes – due Thurs 10/10

Homework:

Read Ch 3.6 and do review questions

Read Summary and Lab Practices pgs 92-93

Pipetting Accuracy Lab parts 1 and 2 - due Tues, 10/8

Ch 3 Test – Thurs 10/10

Finish Math Problems

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Tuesday, Oct 8

In class:

Correct Ch 3.3-3.6 review questions and Math Problems

Ch 3 Test on Tues, 10/15

Continue Pipetting Accuracy Lab, part 2

Go over Math problems

Lab Activity: Micro-pipetting Lab with colored dyes – due Thurs, 10/17

Homework:

Study for Ch 3 Test – Test on Tues, 10/15

Finish Biotech Live – Activity 3.1-3.4 pgs 95-97 and Math problems

Pipetting Accuracy Lab, parts and 2 – due Tues, 10/15

Micro-pipetting Lab with colored dyes – due Thurs, 10/17

Thursday, Oct 10

In class:

Ch 3 Test

Continue with Biotech Live – Activity 3.5-3.6 pgs 98-100

Go over Math/MW of compounds

Activity: more practice on micro-pipetting

Homework:

Study for Ch 3 test - Test on Tues, 10/15

Read Ch 4.1 Studying DNA and do review questions

Pipetting Accuracy Lab, parts and 2 – due Tues, 10/15

Micro-pipetting Lab with colored dyes – due Thurs, 10/17

M/W P1, P3, P5, P7

T/Th P2, P4, P6, P7

F P1 - P7    Minimum Day 

Tuesday, Oct 15

In class:

Go over Math Problems, Ch 3.5-3.6 review questions

Ch 4.1 DNA Structure

Lab Activity: Isolation of Strawberry DNA 

Due today: Pipetting Accuracy parts 1 and 2

Homework:

Read Ch 4.2 Sources of DNA, do review questions

Do Biotech Online: Know your Genome pg 112, Baldness Gene pg 118

Download Amgen Lab Manual – Read ABE Lab 1 Gel Electrophoresis and do prelab notes

Strawberry DNA Lab – due on Thurs, 10/24

Ch 3 Test on Thurs, 10/17

Thursday, Oct 17

In class:

Ch 3 Test and Ch 3 packet due today

Ch 4.2 Sources of DNA, correct review questions

Activity: Cast a Gel for Gel Electrophoresis; make 1x SB buffer and 1% agarose gel; 

- ABE Lab 1 – Gel Electrophoresis

Due today: Micropipetting Lab with red dye

Homework:

Read Ch 4.3 Isolating and Manipulating DNA

Do Biotech Online Recombinant Pharmaceuticals and Therapies, pgs 118-119

Read Lab 2 Amgen Lab Manual – pKan and pARA Digest with restriction enzymes

Strawberry DNA Lab – due on Thurs, 10/24

ABE Lab 1 – Gel Electrophoresis – due on Tues, 10/29

M P1-P7 Banking Day

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, Jan 9

In class:

Review Ch 5 Proteins and PAGE, Polyacrylamide Gel Electrophoresis

Go over Synthetic Biology Project –due in 10 weeks

Assign chapters for group presentations

HW:

Read up on PAGE – protein analysis (Dr. Coba will lecture on how PAGE is used in their lab to analyze proteins

Read Ch 6.1 Assays and Do review questions 6.1; write questions on separate sheet and answer in complete sentences

Brainstorm ideas for Synthetic Biology Project

Click here: https://biobuilder.org/lab/biology-by-design/?a=student

Day 2 Thursday, Jan 11

In class:

Set up PAGE experiment – Fish protein analysis with special guest, Dr. Coba and Mrs. Coba

Go over Synthetic Biology – go to www.Biobuilder.org

Ch 6.1 Potential Products

Lab Activity: PAGE

HW:

Read Ch 6.2 Assays and Do review questions 6.2

PAGE Fish Proteins – Lab report due on Thurs, Jan 18

Brainstorm ideas for Synthetic Biology Project

Click here: https://biobuilder.org/lab/biology-by-design/?a=student

Chapter Assignments: Please prepare a Powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp 6 – Ch 7.2, 7.3

Grp 7 – Ch 8.1-8.5

Grp 8 – Ch 9.1-9.5

Grp 1,2,3 – Ch 12

Grp 6,7,8 – Ch 13

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 1/16

In class:

Review PAGE – staining and destaining gel

Review Ch 6.1 Potential Biotech Product and Ch 6.2 The use of Assays, correct review questions

Group 1 Presentation - Ch 6.3 ELISA, correct review questions

Lab Activity – Finish destaining PAGE gel; Rapid ELISA, TRU RSV - Due on Tues 1/23

HW:

Read Ch 6.3 ELISA and do review questions 6.3

Do Biotech Online ELISA Technology in Diagnostic Kits pg 173

PAGE Lab report – due on Tues, 1/23

Due on Thurs, 1/23 – Rapid ELISA, TRU RSV

Day 2 Thursday, 1/18

In class:

Review Ch 6.2 Assays

Group 1 Presentation - Ch 6.2 The use of Assays, and Ch 6.3 ELISA, correct review questions

Lab Activity: Lab 6b and c Assaying for Starch, Sugar and Amylase Activity – due Tue 1/30

HW:

Read ELISA Lab

Finish Lab 6c Amylase Activity – due Thurs 1/30

Edpuzzle – ELISA

Continue working on Synthetic Biology Project

Chapter Assignments: Please prepare a power point presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp 6 – Ch 7.2, 7.3

Grp 7 – Ch 8.1-8.5

Grp 8 – Ch 9.1-9.5

Grp 1,2,3 – Ch 12

Grp 6,7,8 – Ch 13

M P1-P7 Banking Day

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 1/23

In class:

Review Ch 6.3 ELISA and Amylase

Lab Activity: Lab 6b Assay for Starch and Sugar and Lab 6c Amylase Lab

HW:

Read ELISA Lab – Lab on Thursday

Read Ch 6.4 Western Blots and do review questions

Lab 6b Assay for Starch and Lab 6c Assaying for Amylase Activity – due Tues 1/30

Day 2 Thursday, 1/25

In class:

Review Lab 6c Amylase

Group Presentation – Ch 6.4 Western Blot

Lab Activity: Anti-Heparin ELISA Assay

HW:

Read Ch 6.5 Looking for new products

Do B.O. pg 178 Amazon Hide and Seek

Lab 6b Assay for Starch and Lab 6c Assaying for Amylase Activity – due Tues 1/30

Continue working on Synthetic Biology Project

Bring Herbs for Tuesday and read Lab 6d Looking for Potential Medicine

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp 1 - Ch 6.1-6.3

Grp 2 – Ch 6.4-6.6

Grp 3 – Ch 7.1, 7.4, 7.5

Grp 6 – Ch 7.2, 7.3

Grp 7 – Ch 8.1-8.5

Grp 8 – Ch 9.1-9.5

Grp 1,2,3 – Ch 12

Grp 6,7,8 – Ch 13

M P1-P7 Banking Day

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 1/30               Stem Cell PCC Module: Feb 6-Feb 20

In class:

Review Ch 6.3 and 6.4 Western Blot and go over ELISA Lab

Group presentation – Ch 6.4 and 6.5 Looking for new products

Due today: Lab 6g HRP Isolation, Lab 6h Confirm HRP with TMB

Lab Activity – Lab 6h Confirm HRP with TMB

Lab 6d Looking for new products, continue on Thurs

HW:

Read Ch 6.6 Producing rDNA and do review questions

Read ELISA Anti-Heparin Lab – lab on Thursday 2/1

Lab 6g HRP Isolation – due on Tues 2/6

Day 2 Thursday 2/1

In class:

Lab Activity: ELISA Anti-Heparin Lab

Group Presentation – Ch 6.6 Producing rDNA and correct review questions

Lab Activity: ELISA Anti-Heparin Lab

HW:

Do Biotech Live Activity 6.1-6.4 – due on Thurs 2/8

Read Lab 6d Looking for new products – Do on Tues

Ch 6 Test – Thurs 2/8; Ch 6 review questions - due on Thurs 2/8

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

M P1-P7 Banking Day

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 2/6 (STEM CELL MODULE POSTPONED UNTIL MARCH)

In class:

Go over Ch 6.4 Western Blot

Group Presentation – Ch 6.5 Looking for New Products, do review questions

Lab Activity: Lab 6d Looking for new products – bring herbs

HW:

Read Ch 6.6 Producing rDNA and do review questions

Do Biotech Online pg 163 Quality Not and Quantity

Finish Lab 6g and 6h Isolation of HRP and testing against TMB (on same lab report)- due on Tues 2/8

Day 2 Thursday 2/8

In class:

Group Presentation – Ch 6.6 Producing rDNA and correct review questions

Due today: Lab 6g and 6h Isolation of HRP and testing against TMB (on same lab report)-

Lab Activity: Continue Lab 6d Looking for new products, test against bacteria

HW:

Complete

Finish Biotech Live Activity 6.1-6.4 – due on Tues 2/13

Ch 6 Test – Tues 2/13; Ch 6 review questions-  due on Tues 2/13

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 2/13

In class:

Go over Ch 6 and correct all assignments

Ch 6 Assay Test; Ch 6 packet – due today

Lab Activity: Lab 6d Looking for new products - make final observations, lab report due on Tues 2/20

HW:

Read Ch 7.1 Spectrophotometer and do review questions pgs 195-197

Do B.O. 7.1 FTIR, pg 200

Edpuzzle: Spectrophotometer – take notes; turn in on Tue 2/20

Read Lab 7a and 7b How to Use a Spectrophotometer

Day 2 Thursday, 2/15

In class:

Go over Lab 6d Antimicrobial properties

Group presentation on Ch 7. 1 Spectrophotometer

Lab Activity - Lab 7a and 7b How to Use a Spectrophotometer

HW:

Read Ch 7.4 and 7.5 Use of Spectrophotometers to measure Protein Concentration

Do 7.4 and 7.5 Review questions

Finish Lab 7a and 7b lab reports – due on Thurs 2/22

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 2/20

In class:

Review Ch 7.1 Spectrophotometers

Review Labs 7a and 7b How to use a spectrophotometer

Lab Activity: Soda Lab using the Spectrophotometer; create excel file, convert %T to A

Due today: Lab 6d Looking for Potential Medicine lab reports

Quiz on Ch 7.1 Spectrophotometer

HW:

Read Ch 7.4 and 7.5 Use of Spectrophotometers to measure Protein Concentration

Do 7.4 and 7.5 Review questions

Finish Lab 7a and 7b lab reports – due on Thurs, 2/22

Soda Lab using the Spectrophotometer lab report – due Tues, 2/27

Day 2 Thursday, 2/22

In class:

Review Spectrophotometers, Soda Lab using the Spectrophotometer and conversion A to %T

Group Presentation today: Ch 7.4 and 7.5 Using Spectrophotometers to measure Concentration

Due today: Lab 7a and 7b lab reports

HW:

Read Ch 7.2 and do review questions

Do B.O pg 203

Soda Lab using the Spectrophotometer lab report – due Tues, 2/27

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 2/27

In class:

Grp presentation 7.5 Using Spectrophotometers to measure Concentration and

Ch 7.2 pH and correct review questions pgs 200-203

Learn how a pH meter electrode works

Lab Activity – pH Lab – due on Tues, 3/5

Due today -- Soda Lab using the Spectrophotometer lab report

HW:

Finish Ch 7.2 review questions and B.O. 200 and 203

Finish pH Lab – due on Tues, 3/5

Do pH problems – due on Thurs, 2/29

Day 2 Thursday, 2/29

In class:

Review 7.2 review questions and B.O. 200 and 203

Go over pH problems

Group presentation on Ch 7. 3 Buffer

Video: Buffer Balancing Acts, https://www.youtube.com/watch?v=7ciO9eO3OQg

HW:

Read Ch 7.3 Buffers and do review questions

Do B.O. pg 208 How and why proteins are purified from cells

Do Biotech Live Activity 7.1 Spectrophotometer and 7.2 Phun at Home with pH

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

M P1-P7 Banking Day

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Fri Minimum day

Day 1 Tuesday, 3/5

In class:

Review Ch 7.3 Buffer

Read Ch 7 Summary and Lab Practices

EP: Buffer Balancing Acts, take notes and take quiz

Due today: pH Lab

HW:

Study Ch 7 Summary and Lab Practices – Ch 7 Test on Thurs 3/7

Do Thinking Like a Biotechnician #1-10 pg 216

Finish Biotech Live Activity 7.1-7.2 pg 216-217

Ch 7 packet – due on Thurs 3/7

Soda Lab – due on Thurs 3/7

Electrophoresis, Buffer, and pH Lab – due on Tues 3/12

Day 2 Thursday, 3/7

In class:

Grp presentation – Ch 7.3 Buffers

Ch 7 Spectrophotometers Test

Ch 7 Packet due today

Due today - Soda Lab

HW:

Read Ch 8.1 The Production of Recombinant Biotech Product; do review questions

Do B.O. Some Say Genetic Engineering.. pg 226

Electrophoresis, Buffer, and pH Lab – due on Tues 3/12

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

M/W P1, P3, P5, P7

T/Th P2, P4, P6, P7

Fri P1-P7 Minimum day

Day 1 Tuesday, 3/12

In class:

Group Presentation: Ch 8.1 The Production of Recombinant Biotech Product; correct review questions

Lab Activity: Lab 1 Gel Electrophoresis to practice pipetting micro-volumes; calculate and pour gel

Work on Synthetic Biology Project – learn how to use Tinker Cell

HW:

Read Ch 8.2 Transforming Cells pgs 227-234 and do review questions

Do B.O. BACs and YACs pg 239; Endonucleases pg 230; RFLPs pg 231

Read ABE Lab 2 Restriction Digest pGrn and pDrk with HindIII and Lab 3 Ligation

Continue working on Synthetic Biology Project

Day 2 Thursday, 3/14

In class:

Grp presentation – Ch 8.2 Transforming Cells and correct review questions

Lab Activity: ABE Lab 2 Restriction Digest - Digest pGrn and pDrk with HindIII

Work on Synthetic Biology and Tinker Cell

HW:

Read ABE Lab 3 Ligation to create a recombinant rpGlo

Complete ABE Lab 2 Restriction Digest pGrn and pDrk  report – due on Tues 3/26

Work on Synthetic Biology project – due on Tues April 2

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

SPRING BREAK

HW from Tuesday, 3/12

Read Ch 8.2 Transforming Cells pgs 227-234 and do review questions

Do B.O. BACs and YACs pg 239; Endonucleases pg 230; RFLPs pg 231

Read ABE Lab 2 Restriction Digest pGrn and pDrk with HindIII and Lab 3 Ligation

Continue working on Synthetic Biology Project

HW from Thursday, 3/14

Read ABE Lab 3 Ligation to create a recombinant rpGlo

Complete ABE Lab 2 Restriction Digest pGrn and pDrk  report – due on Tues 3/26

Work on Synthetic Biology project – due on Tues April 2

If you have not turned in your lab reports, please post them soon! Thanks!

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Ch 8.2 and 8.3 Don't forget to make a ppt presentation ready to present on Tuesday

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Have a restful Spring Break!

M Professional Development; No students on campus

T/Th P1, P3, P5, P7 (Thursday – Health/Wellness Assembly)

W/Fri P2, P4, P6, P7

Day 1 Tuesday, 3/26

In class:

Review ABE (Green) Lab 2 Restriction Digest of pDrk and pGrn

Lab Activity: ABE (Green) Lab 3 Ligation of cut pDrk and pGrn – lab report due Tues, 4/2

Group Presentation – Ch 8.2 Transformation and review questions

Work on Synthetic Biology

Due today: ABE Lab 2 Restriction Digest of pDrk and pGrnf

HW:

Review Ch 8.2 Transforming Cells

Finish B.O. BACs and YACs pg 239; Endonucleases pg 230; RFLPs pg 231

Read Ch 8.3 After Transformation and do review questions

Do B.O pg 238 You can Try This at Home

Read ABE (Green) Lab 5 Transformation

Day 2 Thursday 3/28

In class:

Review ABE (Green) Lab 3 Ligation to construct rpGLO

Review Transformation, Ch 8.2 and Lab 5 Transformation

Lab Activity: ABE (Green) Lab 5 Transformation of HB101 with rpGLO - lab report due Tues, 4/2

HW:

ABE Labs 3 Ligation to construct rpGLO – lab report due Thurs, 4/4

Read Ch 8.4 Fermentation, Manufacturing, and GMP and do review questions

Read ABE(Green) Lab 4 Confirmation of Restriction Digest of pGRN and pDRK

Synthetic Biology Project, Group presentations begin on Tues, 4/9

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp 1,2,3,6,7,8 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 4/2

In class:

Review ABE (Green) Lab 5 Transformation of HB101 with rpGLO - lab report due today, Tues, 4/2

Grp Presentation - Ch 8.4 Fermentation, Manufacturing, and GMP and do review questions

and Ch 8.3 After Transformation and do review questions

Lab Activity: Lab 4 Confirmation of Digest of pDrk and pGrn and do prelab notes,

Continue Lab 5 Transformation – look for green glowing cells

HW:

ABE Labs 3 Ligation to construct rpGLO – lab report due Thurs, 4/4

ABE (Green) Lab 5 Transformation of HB101 with rpGLO - lab report due Tues, 4/9

ABE Lab Activity: Lab 4 Confirmation of Digest of pGRN and pDrk, due Thurs, 4/11

Read Ch 8.5 Retrieving Plasmids after Transformation

Day 2 Thursday, 4/4

In class:

Review ABE Lab 4 Confirmation of Digest of pDrk and pGrn

Grp Presentation - Ch 8.5 Retrieving Plasmids after Transformation correct review questions

Do Biotech Live 8.1-8.4 pgs 248-250

Due today:  ABE Labs 3 Ligation to construct rpGLO lab report

HW:

Finish Biotech Live 8.1-8.4 pgs 248-250 and all assignments

ABE (Green) Lab 5 Transformation of HB101 with rpGLO - lab report due Tues, 4/9

ABE Lab Activity: Lab 4 Confirmation of Digest of pGRN and pDrk, due Thurs, 4/11

Ch 8 Test on Tuesday, 4/9; Ch 8 packet due 4/9

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 4/9

In class:

Review Ch 8 and ABE Labs 1-5

Ch 8 test today

Lab Activity: ABE Lab 4 Confirmation of restriction digest

Due today: Ch 8 packet includes review questions, B.O. 8.1-8.5, and Biotech Live 8.1-8.5

Due today: ABE (Green) Lab 5 Transformation of HB101 with rpGLO - lab report

Set up culture for Mini-prep

HW:

Read Ch 9. 1 Bringing Product to Market, do review questions

Read Mini-prep lab - Lab on Thursday

Day 2 Thursday 4/11

In class:

Group presentation: Ch 9.1 Bringing Product to Market

Lab Activity: Mini-prep lab

HW:

Finish Mini Prep lab – lab report due on Thurs 4/18

Read Ch 9.2 Using Chromatography to study molecules and do review questions

Get ready for DNA Barcoding – read isolating DNA from bees

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

M P1-P7

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 4/16

In class:

Go over ABE Lab 4 Confirmation of Restriction Digest

Review Ch 9.1 Bringing Product to Market

Group Presentation – Ch 9.2 Using Chromatography to study molecules

Lab Activity: Mini-prep

HW:

ABE Lab 4 Confirmation of Restriction Digest Lab Report – due on Thurs 4/18

Read Ch 9.3 Column Chromatography: An expanded discussion; do review questions

Do B.O. Got Gas pg 268

Mini-prep lab report – due Tues, 4/23

Day 2 Thursday, 4/18

In class:

Review Ch 9.2 Chromatography and mini-prep

Group Presentation – Ch 9.3 Column Chromatography

Lab Activity: Column Chromatography

HW:

Finish Mini-prep lab report – due Tues, 4/23

Column Chromatography lab report – due Thurs, 4/25

Read Ch 9.4 Product Quality Control and do review questions

Do B.O. Approved Biotechnology Drugs pg 273

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

M/Th P1, P3, P5, P7

Wednesday – No School

W/F P2, P4, P6, P7

Day 1 Tuesday, 4/23

In class:

Intro to DNA Barcoding

DB Lab 1 and Lab 2 – Isolate DNA from Bees

Due today: ABE Lab 4 Confirmation of Digest

HW:

Read Intro to DNA Barcoding: DB Lab 1 and Lab 2 – Isolate and purify DNA from Bees

Do Ch 9 Biotech Live Activity 9.1-9.3

Ch 9 Test on Tues 4/30

Day 2 Thursday 4/25

In class:

Go over Purifying DNA from Bees

Lab Activity: DB Lab 2 Purify DNA from Bees

HW:

Read DB Lab 3 Casting Gel and DB Lab 4 Gel Electrophoresis

Read Ch 9 Summary and Lab Practices

Ch 9 Test on Tues 4/30

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 4/30

In class:

Review DB Labs 1 and 2 Purifying gDNA

Lab Activity DB Lab 1 and Lab 2 – Purifying total DNA (gDNA) from tissue lysates

Ch 9 Test today

HW:

DB Labs 1 and 2 Isolate and Purify DNA from Bees – due Thursday 5/9

Read DB Lab 3 and 4 Examining gDNA using Gel Electrophoresis

Day 2 Thursday 5/2

In class:

Go over Purifying DNA from Bees using spin column (chromatography)

Go over Chromatography

Lab Activity: DB Lab 3 and 4 Examining gDNA using Gel Electrophoresis

HW:

Read DB DB Lab 5 Targeted amplification of the COI barcode region from a gDNA template

DB Labs 1 and 2 Isolate and Purify DNA from Bees lab report – due Thursday 5/9

DB Lab 3 and 4 Examining gDNA using Gel Electrophoresis lab report – due on Tue, 5/14

Read DB Lab 5 Targeted amplification of the COI barcode region from a gDNA

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Monday – P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 5/7

In class:

Go over DB Labs 3 and 4 Confirming gDNA by gel electrophoresis

Lab Activity: DB Labs 3 and 4 Confirming gDNA by gel electrophoresis

HW:

DB Labs 1 and 2 Isolate and Purify DNA from Bees lab report – due Thurs 5/9

DB Labs 3 and 4 Confirming gDNA by gel electrophoresis – due Thurs 5/16

Read DB Lab 5 Amplification of CO1 by PCR

Day 2 Thursday 5/9

In class:

Go over DB Lab 5 Targeted amplification of the COI by PCR

Lab Activity: DB Lab 5 Amplification of CO1 by PCR

Due today: DB Labs 1 and 2 Isolate and Purify DNA from Bees lab report

HW:

Read DB Lab 6: Spin Column to purify CO1 amplicons

DB Labs 3 and 4 Confirming gDNA by gel electrophoresis – due Thurs 5/16

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp8 – Ch 12

Grp9 – Ch 13

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 5/14

In class:

Lab Activity today: DB Lab 5 CO1 Amplification

Go over DB Lab 3 and 4 Spin Column to purify gDNA and confirm by gel electrophoresis

HW:

DB Labs 3 and 4 Confirming gDNA by gel electrophoresis lab report – due Thurs 5/16

Read DB Lab 6 Spin Column to purify CO1 gene

DB Lab 5 CO1 Amplification lab report – due Thurs 5/23

Synthetic Biology Project due on Thurs 5/23

Day 2 Thursday 5/16

In class:

Go over DB Lab 5 CO1 Amplification

Due today: DB Labs 3 and 4 Confirming gDNA by gel electrophoresis lab report

Lab Activity: DB Lab 6 Spin Column to purify CO1 gene

HW:

Read DB Lab 7 and 8 Gel Electrophoresis to Confirm CO1

DB Lab 5 CO1 Amplification lab report – due Thurs 5/23

Read Ch 12.1 Medical Biotechnology Synthesis and do review questions

Do Ch 12.1 B.O. New Medications and Penicillin pgs 344-345

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp 1,2,3 – Ch 12

Grp 6,7,8 – Ch 13

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 5/21

In class:

Go over DB Lab 6 pin Column to purify CO1 gene – due Thurs 5/23

Lab Activity today: B Lab 7/8 Confirmation of CO1 by Gel Electrophoresis

Group Presentation – Ch 12.1 Drug Discoveries

HW:

Read Ch 12.1-12.2 Medical Biotechnology and Pharmaceuticals Synthesis and do review questions

Do Ch 12.1 B.O. New Medications and Penicillin pgs 344-345

DB Lab 7/8 Confirmation of CO1 by Gel Electrophoresis – due on Tues, 5/28

Day 2 Thursday 5/23

In class:

Go over DB Lab 7/8 Confirmation of CO1 by Gel Electrophoresis

Group Presentation - Ch 12.2 Creating Pharmaceuticals and 12.3 Creating Pharmaceuticals

Due today: DB Lab 6 pin Column to purify CO1 gene – due Thurs 5/23

HW:

Read 12.3 Creating Pharmaceuticals; do review questions

Read Ch 12.4 Creating Pharmaceuticals by Peptide/Antibody Engineering and do review questions

Do B.O. Getting Sick is No Laughter pg 354 and B.O. Eating Vegetables pg 355

DB Lab 7/8 Confirmation of CO1 by Gel Electrophoresis – due on Tues, 5/28

Study for LAMC finals – Finals: Friday, 5/29

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp 1,2,3 – Ch 12

Grp 6,7,8 – Ch 13

Monday – Memorial Day Holiday – No School

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, May 28

In class:

Group Presentation on Ch 12.3 Creating Pharmaceuticals

Presentation on Synthetic Biology Project

HW:

Finish your Career Exploration Project – presentations on Thursday, May 30

Finish your resume

Study for Finals Ch 1-9, 12 - Finals on Monday, June 3

Day 2 Thursday May 30

In class:

Career Exploration Project – presentations on today

Due today: Resume

HW:

Study for Finals – Finals on Monday, June 3

M P1-P7 Banking Day

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Tuesday, Oct 17

In class:

Go over 4.3 DNA Structure and Isolation of DNA

Quiz on Ch 4.1-4.3

Go over ABE Lab 1 Gel Electrophoresis

Lab Activity: ABE Lab 2 Restriction Digest of pKan and pAra

Due today: Strawberry DNA Isolation Lab

Homework:

Read Ch 4.4 Gel Electrophoresis and do review Questions

Read ABE Lab 3 Ligation

Finish ABE Lab 2,3 Prelab Notes

Do B.O. Chop and Go Electrophoresis pg 124

Do Ch 4 Biotech Live 4.1-4.2

ABE Lab 1 Gel Electrophoresis – due on Tuesday 10/24

Thursday, Oct 19

In class:

Go over Ch 4.4 Gel Electrophoresis

Quiz on Gel Electrophoresis

Go over ABE Lab 2 Restriction Digest of pKan and pAra

Lab Activity: ABE Lab 3 Ligation

Homework:

Do Biotech Online Chop and Go pg 124

Finish ABE Lab 1 Gel Electrophoresis – due on Tuesday 10/24

Read ABE Lab 4 and 5 Confirmation of Digest and Transformation of HB101 with pAra-R

Do ABE Lab 4,5 Prelab Notes

Read Ch 4 Summary and Lab Practices pgs 126-127

Do Ch 4 Biotech Live 4.4-4.6

Ch 4 Test on Thursday, 10/26

M/Th P1, P3, P5, P7

W PSAT 8:30-12:30

T/F P2, P4, P6, P7

Tuesday, Oct 9

In class:

Go over Math Problems

Ch 4.1 DNA Structure

Lab Activity: Isolation of Strawberry DNA

Due today: Student-written procedure on micropipetting  with red dye; Math Problems

Homework:

Read Ch 4.2 Sources of DNA, do review questions

Do Biotech Online: Know your Genome pg 112, Baldness Gene pg 118

Download Amgen Lab Manual – Read ABE Lab 1 Gel Electrophoresis and do prelab notes

Strawberry DNA Lab – due on Tues, 10/17

Thursday, Oct 11

In class:

Ch 4.2 Sources of DNA, correct review questions

Activity: Cast a Gel for Gel Electrophoresis; make 1x SB buffer and 1% agarose gel;

- ABE Lab 1 – Gel Electrophoresis

Homework:

Read Ch 4.3 Isolating and Manipulating DNA

Do Biotech Online Recombinant Pharmaceuticals and Therapies, pgs 118-119

Read Lab 2 Amgen Lab Manual – pKan and pARA Digest with restriction enzymes

Strawberry DNA Lab – due on Tues, 10/17

Date Assigned: 08/28/2023Date Due: 08/28/2023

M     -   P1-P7 (Banking Day)

T/Th - P1, P3, P5, P7

W/F - P2, P4, P6, P7

Tuesday, August 29

In Class:

What is Scientific Method?

Correct Ch 1.2-1.3 review questions

Lab Activity: Cheese Lab, make first observation, record in your notebook

Biotech Live:  Activity 1 What is Biotechnology? pgs 32-33

Activity 2 Business Side pgs 33-34

Bleach Lab due today– Did you send to intern to check for content/format?

HW:

Read ED Ch. 1.4 and review questions pgs 19-23 ED

Do B.O. Hot Jobs pg 26

Extra Credit: make cheese at home; DIY on foodwishes.com

Thursday, August 31

In Class:

Cheese Lab - Make ^2nd observation, record in your notebook ready for cheese cloth?

Discuss Careers in Biotech

Do Biotech Live Activity 1.4 How Biotech Industry Improving Human Life? pg 35

Correct Ch 1.4 review questions

HW:

Read ED Ch 1.5-1.6 and do review questions pgs 23-29 ED

Do B.O. Code of Business Conduct pg 29 ED

Read Summary and Lab Practices pgs 30-31

Please share your lab report with your intern to get it checked before turning it in to me.

Biotech Interns: Here are your interns:

Caris (ckim7961@stu.gusd.net):Last names A-Hovanes
Ani (ahar1308@stu.gusd.net): Last names I-Markarian
Irene (ikim5843@stu.gusd.net) and Emily (egai0346@stu.gusd.net): Last names Montecello-V

You can also get help from Stella (ster9435@stu.gusd.net) and Emi (enak1491@stu.gusd.net)

Date Assigned: 08/21/2023Date Due: 08/21/2023

M     -   P1-P7 (Banking Day)

T/Th - P1, P3, P5, P7

W/F - P2, P4, P6, P7

Day 1 Tuesday, Aug 22

In class:

What is Biotech?

Go over How to maintain a Scientific Notebook in Biotech

Lab Activity: Bleach Lab pgs 19-21 TB

HW:

Read Ch 1.1-1.2 and do review questions Ch 1.1-1.2 in ED - write questions and answers on paper with black ink

Read Cheese Lab before Thursday and bring whole milk and buttermilk on Thursday

Do Biotech Online, B.O., pg 13 Glo Fish

Day 2 Thursday, Aug 24

In class:

Complete and make final observations for Bleach Lab, lab report due on Th 8/31

Review How to Notebook and how to write an effective conclusion

Lab Activity: Cheese Lab

HW:

Read Ch 1.3 pgs 14-18 and do review questions 1.3 ED

Do Biotech Online (B.O.) pg 18 Genentech

Finish Bleach Lab report in Google Docs pgs 19-21 ED; lab report due on Th 8/31

Date Assigned: 08/15/2023Date Due: 08/15/2023

Day 1 Wednesday, August 16

In class:

Rules and Policies

Safety Rules

HW:

Sign up for Google Classroom, Quizizz, Edpuzzle

Study for Safety Test – Test on Thursday

Wear shoes to class – Always!

Day 2 Thursday, August 17

In class:

Safety Test

What is Biotechnology

HW:

Read Ch 1.1 Biotechnology ED (Ellyn Daugherty textbook)

Read Bleach Lab pgs 19-21 ED, Lab on Tuesday

Wear shoes, = please!

M/W P1, P3, P5, P7

T/Th P2, P4, P6, P7

F No School

Tuesday, Oct 24

In class:

Go over ABE Lab 3 Ligation of pAra and pKan

Lab Activity today: ABE Lab 5 Transformation

Do/Finish CTE Modules 2 & 3

Due today: ABE Lab 1 Gel Electrophoresis

Homework:

Read ABE Lab 5 Transformation and Lab 4 Confirmation of pAra and pKan

Finish Biotech Live 4.1-4.6

Do/Finish CTE Modules 2 & 3

Study for Ch 4 Test – Test on Thursday, 10/26

Thursday, Oct 26

In class:

Ch 4 Test

Go over ABE Lab 5 Transformation Ligation and Lab 4 Confirmation of pAra and pKan

Lab 4: Lab 4 Confirmation of pAra and pKan by Gel Electrophoresis

Homework:

Do/Finish CTE Modules 2 & 3

Due: ABE Lab 4 Confirmation of Restriction Digest by Gel Electrophoresis

Finish ABE Labs 2 and 3 report – due Thursday, 11/2

Finish Ch 4 Packet – Review Questions Ch 4.1-4.4, Biotech Online 4.1-4.4, Biotech Live 4.1-4.6 – due on Tuesday, 11/2

M/W P1, P3, P5

T/Th P2, P4, P6, P7

F P1-P7 Shakeout Assembly Schedule

Tuesday, Oct 31

In class:

Review ABE Labs 2-4 –Restriction Digest of pAra and pKan-R and Ligation

Lab Activity: ABE Lab 5 Transformation of E.coli with pAra-R

ABE Labs 2 and 3 lab reports due on Thursday, 11/2

Due today: Ch 4 Packet

Quiz on ABE Labs 1 -3

Homework:

Read ABE Lab 5 Transformation of E.coli with pAra-R

Complete ABE Labs 2 and 3 lab reports due on Thursday, 11/2

ABE Lab 4 Confirmation – Due Tue 11/7

Finish Ch 4 Packet – due Thurs 11/2

Thursday, Nov 2

In class:

Finish ABE Lab 5 – screen for red colonies and go over Bacterial Transformation

Hands-on Activity – HASPI Diagnosing Genetic Disease Through Gel Electrophoresis

Bioinformatics NCBI and Blast – If time permits or we will do on Tue 11/7

Due today: ABE Labs 2 and 3 lab reports and Ch 4 packet

Quiz on Restriction digest and Gel Electrophoresis

Homework:

Finish Activity on Diagnosing Genetic Disease Through Gel Electrophoresis – Due on Tue 11/7

Read ABE Lab 6 Overnight Culture of Transformed E.coli

Finish ABE Labs 4 Confirmation of Digest lab report – Due Tue 11/7

M/W P1, P3, P5, P7

T/Th P2, P4, P6, P7

Friday – Veteran's Day, No School

Tuesday, Nov 7

In class:

Review ABE Lab 6 Set up Overnight Culture of Transformed Cells

Lab Activity today – ABE Lab 7 Isolation of RFP by Column Chromatography

Quiz on ABE Lab 4, 5 Confirmation, and Transformation

Due today: ABE Lab 4 Confirmation of Digest lab report

Homework:

Read Ch 5.1 Protein structure and function and do review questions

B.O. Antibody Producing Companies

ABE Lab 5, Transformation due Thursday, 11/9

Read ABE Lab 7 Isolation of RFP by Column Chromatography; do prelab questions

Thursday, Nov 9

In class:

Go over ABE Lab 7 Isolation of RFP by Column Chromatography;

Activity: Paper Plasmid – constructing recombinant DNA

Due today: ABE Lab 5, Transformation

Homework:

Finish ABE Lab 7 Isolation of RFP by Column Chromatography lab report – due Thursday, 11/16

Read Ch 5.2 Protein Production and Protein Activity

B.O. Couch Potatoes pg 146

M –  Banking Day, P1-P7

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Tuesday, Nov 14

In class:

Ch 5.1 and 5.2 Protein Structure and Function

Go over HASPI Diagnosing Genetic Disorders by Electrophoresis – due today

Activity – Paper recombinant plasmid construction

Quiz Ch 5.1 Protein Structure

Homework:

Read Ch 5.3 Protein Catalysts and do review questions

B.O. Catalysts for Better Health pg 151

ABE Lab 6/7 Isolation of RFP by Column Chromatography – due on Thurs 11/16

Thursday, Nov 16

In class:

Ch 5.2 and 5.3 Protein Production and Protein Activity; correct review questions

Activity: Genetic Disorders from NCBI/BLAST activity – Presentations will be on Tues, 11/28

Due today: ABE Lab 6/7 Isolation of RFP by Column Chromatography

Homework:

Read Ch 5.4 Studying Proteins and Ch 5.5 Analyzing Proteins and do review questions

B.O. Protein Sequencers – pg 156

Genetic Disorders from NCBI/BLAST activity – Presentations will be on Tues, 11/28

HAPPY THANKSGIVING!

Thanksgiving Week – No School

Reminder: Read Ch 5.4 Studying Proteins and Ch 5.5 Analyzing Proteins and do review questions

B.O. Protein Sequencers – pg 156

Genetic Disorders from NCBI/BLAST activity – Presentations will be on Tues, 11/28

Happy Thanksgiving!

M –  Banking Day, P1-P7

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Tuesday, Nov 28

In class:

Review Ch 5.1-5.3 Protein Enzymes and correct assignments

Due today -– Genetic Disorders from NCBI/BLAST activity – Presentations begin today

Lab Activity – Antibody-Antigen Test

Homework:

Read/review Ch 5.1-5.3 more on Proteins

Complete Antibody-Antigen Test lab report – due Tues, 12/5

Thursday, Nov 30

In class:

Review Enzymes and Studying Proteins

Continue Genetic Disorders Presentations

Lab Activity – Catalase Lab, parts A-D

Homework:

Graph results of Catalase Lab

Read PAGE Lab (Polyacrylamide Gel Electrophoresis)

Review Ch 5.4 and Study for Ch 5 Test

Ch 5 Test – Thurs, 12/7

M –  Banking Day, P1-P7

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Tuesday, Dec 5

In class:

Due today -– Antibody-Antigen: HCG and iFOBT (Occult Blood) Lab reports

Correct Ch 5.3-5.4 Protein Analysis

Go over Enzymes and studying proteins

Catalase Lab – Lab report due Tues 12/12

Continue with Genetic Disorder Presentations

Homework:

Study for Ch 5 Test and Complete Ch 5 Packet – Test on Thurs 12/7

Read Ch 5.5 Applications of Protein Analysis and PAGE

Read Ch 5 Summary and Lab Practices pgs 158-159

Do Biotech Live Activity 5.1-5.3

Thursday, Dec 7

In class:

Go over Graphing Data from Catalase; Continue Catalase Lab

Review Ch 5 Proteins, Enzymes

Ch 5 Test

Homework:

Finish Catalase Lab – due Tues, 12/13

Read PAGE Polyacrylamide Gel Electrophoresis – do on Tues, Dec 12

Study for Finals, Ch 1-5 – Finals on Thurs 12/14                          

Date Assigned: 06/05/2023Date Due: 06/05/2023

M Finals in P1, P2

Review in P3, P4

T Finals in P3, P4

Review in P5, P6

W Finals in P5, P6

Please bring back your textbook to room 2205. We need it for our summer class.

DO NOT BRING IT TO THE LIBRARY.

No meeting this week for Biotech

Have a Great Summer Vacation!

Date Assigned: 05/29/2023Date Due: 05/29/2023

Monday – Memorial Day Holiday – No School

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Wednesday, 5/31

In class:

DNA Barcoding: Bioinformatics; Look at Trace Files and barcodes, enter metadata

Group Presentation on Ch 12.3 Creating Pharmaceuticals

HW:

Study for Finals Ch 1-9, 12 - Finals on Friday, 6/2

Day 2 Thursday 6/1

In class:

DNA Barcoding: Bioinformatics; Finish editing and uploading Barcodes

HW:

Read DB Lab 7 Confirmation of CO1 Amplicons

Study for Finals – Finals on Friday, 6/2

Day 3 Friday, 6/2

In class:

Finals

Date Assigned: 05/22/2023Date Due: 05/22/2023

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 5/23

In class:

Group Presentation – Ch 12.1 Drug Discoveries

DNA Barcoding – go over PCR

HW:

Read Ch 12.2 Creating Pharmaceuticals and 12.3 Creating Pharmaceuticals; do review questions

Start studying for LAMC finals

Day 2 Thursday 5/25

In class:

Group Presentation - Ch 12.2 Creating Pharmaceuticals and 12.3 Creating Pharmaceuticals

HW:

Read Ch 12.4 Creating Pharmaceuticals by Peptide/Antibody Engineering and do review questions

Do B.O. Getting Sick is No Laughter pg 354 and B.O. Eating Vegetables pg 355

Study for LAMC finals

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp 1,2,3 – Ch 12

Grp 6,7,8 – Ch 13

Date Assigned: 05/15/2023Date Due: 05/15/2023

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 5/16

In class:

Due today: DB Lab 5 CO1 Amplification

DB Lab 7 and 8 Gel Electrophoresis to Confirm CO1

HW:

Read Ch 9 Summary and Lab Practices

Study for Ch 9 Test – Test on Thursday

DB Lab 6 Spin Column to purify CO1 gene - due on Thurs

Day 2 Thursday 5/18

In class:

Due today: DB Lab 6 pin Column to purify CO1 gene

Ch 9 Test today

Go over Bioinformatics

HW:

Read Ch 12.1 Medical Biotechnology Synthesis and do review questions

Do Ch 12.1 B.O. New Medications and Penicillin pgs 344-345

DB Lab 7/8 Confirmation of CO1 by Gel Electrophoresis – due on Tues, 5/23

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 05/08/2023Date Due: 05/08/2023

Monday – P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 5/9

In class:

Review DB Labs 3 and 4 Confirming gDNA by gel electrophoresis

Lab Activity: DB Lab 5 Amplification of CO1 by PCR

Due today: DB Labs 1 and 2 Isolating and purifying gDNA

HW:

DB Labs 3 and 4 Confirmation by gel electrophoresis – due Thursday 5/11

Read DB Lab 6 Spin Column to purify CO1 Amplicon

Finish Ch 9.4 Using Chromatography to Study Molecules and do review questions

Do B.O. Pipeline pg 270

Read Ch 9.5 Marketing and Sales, do review questions

Do B.O. Biotech Drugs pg 273

Day 2 Thursday 5/11

In class:

Go over DB Lab 5 Targeted amplification of the COI by PCR

Lab Activity: DB Lab 6: Spin Column to purify CO1 amplicons

Due today: DB Lab 3 and 4 Confirmation of gDNA by gel electrophoresis

HW:

Read DB Lab 7 and 8 Confirmation of COI amplicons by gel electrophoresis

Finish all Biotech Live Ch 9.1-9.3 pg 276-277 and all B.O.s

DB Lab 5 Amplification of CO1 lab report – due on Tues, 5/15

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 05/01/2023Date Due: 05/01/2023

Monday – P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 5/2

In class:

Review DB Labs 1 and 2 Purifying gDNA

DB Lab 1 and Lab 2 – Isolate DNA from Bees

Lab Activity: DB Lab 3 and 4 Examining gDNA using Gel Electrophoresis

HW:

DB Labs 1 and 2 Isolate and Purify DNA from Bees – due Thursday 5/4

Read Ch 9.3 Column Chromatography: An Expanded Discussion, do review questions

Do Ch 9.3 B.O. Got Gas pg 268

Read DB Lab 5 Targeted amplification of the COI barcode region from a gDNA template

Day 2 Thursday 5/4

In class:

Go over Purifying DNA from Bees using spin column (chromatography)

Go over Chromatography

Lab Activity: DB Lab 5 Targeted amplification of the COI barcode region from a gDNA template

HW:

Read DB Lab 6 Spin-column purification of COI amplicons

Read Ch 9.4 Using Chromatography to Study Molecules and do review questions

DB Lab 3 and 4 Examining gDNA using Gel Electrophoresis – due on Tue, 5/9

DB Lab 5 Targeted amplification of the COI barcode region from a gDNA template – due Thurs, 5/11

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 04/24/2023Date Due: 04/24/2023

Monday – No School

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 4/25

In class:

Intro to DNA Barcoding

DB Lab 1 and Lab 2 – Isolate DNA from Bees

HW:

Read Ch 9.1 Bringing Product to Market, do review questions

Read Intro to DNA Barcoding: DB Lab 1 and Lab 2 – Isolate and purify DNA from Bees

Day 2 Thursday 4/27

In class:

Go over Purifying DNA from Bees

Lab Activity: DB Lab 2 Purify DNA from Bees

HW:

Read DB Lab 3 Casting Gel and DB Lab 4 Gel Electrophoresis

Read Ch 9.2 Using Chromatography to Study Molecules and do review questions

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 04/17/2023Date Due: 04/17/2023

M/W P1, P3, P5, P7

T/Th P2, P4, P6, P7  Open House

Fri P1-P7 (Minimum Day)

Day 1 Tuesday, 4/18

In class:

ABE Lab 5 Transformation of HB101 with rpGLO

Group Presentation – Ch 8.4 Fermentation and Manufacturing and Ch 8.5 Retrieving Plasmids

HW:

Read Ch 8 Summary and Lab Practices pg 246-248

Do Biotech Live Activity 8.1-8.4 and finish all Ch 5 review questions and Biotech Online

Study for Ch 8 Test – Test on Thurs 4/20

Day 2 Thursday, 4/20

In class:

Ch 8 Test

Lab Activity: Screen for Green Fluorescent Colonies

DNA Barcoding Starts!

HW:

Watch and learn about DNA Barcoding – PPT presentation in GC

Read DB Lab 1 Isolating Genomic DNA from Bees

ABE Lab 5 Transformation – Due on Tues, 4/25

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 04/10/2023Date Due: 04/10/2023

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 4/11

In class:

Due today: ABE Lab 2 and 3 Restriction Digest and Ligation of pDrk and pGrn

Go over ABE Lab 4 Confirmation of Restriction Digest of pGRN and pDRK

Grp Presentation: Ch 8.3 After Transformation and do review questions

Lab Activity: ABE green Labs 5 Transformation of HB101 with rpGLO

HW:

Do B.O. pg 238 You can Try This at Home

Read Ch 8.4 Fermentation, Manufacturing, and GMP and do review questions

Due on Thursday: ABE Lab 4 Confirmation of Restriction Digest of pGRN and pDRK

Synthetic Biology Project, Group presentations begin on 4/21

Day 2 Thursday 4/13

In class:

Due today: ABE Lab 4 Confirmation of Restriction Digest of pGRN and pDRK

Go over ABE Lab 5 Transformation of HB101 with rpGLO

Do Biotech Live: Activity 8.1-8.4 pgs 248-250

HW:

Read Ch 8.5 Retrieving Plasmids after Transformation and do review questions

Finish ABE Lab 5 Transformation of HB101 with rpGLO – due Tues 4/17

EP: Transformation and Mini Prep – take notes and take quiz

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 04/03/2023Date Due: 04/03/2023

M P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 4/4

In class:

Group presentation - Ch 8.1 Recombinant Biotechnology

Set up ABE Lab 2 Restriction Digest of pDrk and pGrn and ABE Lab 3 Ligation

Due in two weeks: Synthetic Biology Project, Group presentations begin on 4/21

HW:

Read Ch 8.2 Transforming Cells and do review questions

Do B.O. BACS and YACS pg 228

Read ABE (green) Labs 2 and 3 Restriction Digest and Ligation of pDrk and pGrn

Read ABE Lab 4 Confirmation of Digest of pDrk and pGrn and do prelab notes

ABE Lab 2 Restriction Digest and Ligation of pDrk and pGrn – lab report due on Tue 4/11

Day 2 Thursday, 4/6

In class:

Group presentation – Ch 8.2 Transforming Cells and correct review questions

Lab Activity: ABE Lab 4 Confirmation of Digest of pDrk and pGrn

HW:

Read Ch 8.3 After Transformation and do review questions

Read ABE green Labs 5 Transformation of HB101 with rpGLO

Due in two weeks: Synthetic Biology Project, Group presentations begin on 4/21

ABE Lab 2 Restriction Digest and Ligation of pDrk and pGrn – lab report due on Tue 4/11

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 03/27/2023Date Due: 03/27/2023

Mon P1-P7 (Banking Day)

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 3/28

Review Ch 7.4 and 7.5

Due today: Lab 7a and 7b Lab reports

Group Presentation – Ch 7.2 pH and Ch 7.3 Buffers, correct review questions

Lab Activity: pH Lab next Tuesday

HW:

Finish Ch 7 review questions, Biotech Live and Biotech Online – due on Thurs 3/30

Read Ch 7 and Lab Practices to get ready for Ch 7 Test

Ch 7 Test on Thurs 3/30

Soda Lab Reports – due on Thurs 3/30

Read ABE Lab 2 Restriction Digest pGrn and pDrk with HindIII and Lab 3 Ligation

Lab Activity - ABE Lab 2 Restriction Digest pGrn and pDrk with HindIII

Day 2 Thursday, 3/30

In class:

Ch 7 Test today

Due today - Ch 7 review questions, Biotech Live and Biotech Online

Due today - Soda Lab Report

Ch 8.1 Overview of Genetic Engineering

Lab Activity: ABE Lab 3 Ligation

HW:

Read Ch 8.1 Overview of Genetic Engineering and do review questions

Read ABE Lab 3 and 4 Ligation and Confirmation of Restriction Digest

ABE Lab 2 and Lab 3 Restriction Digest pGrn and pDrk with HindIII and Ligation – due on Thurs 4/6

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 03/20/2023Date Due: 03/20/2023

Monday – Professional Development; no students

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 3/21

In class:

Review Ch 7.1 Spectrophotometers

Group Presentation today: Ch 7.4 and 7.5 Using Spectrophotometers to measure Concentration

Lab Activity: Soda Lab using the Spectrophotometer; create excel file, convert %T to A

Due today: Lab 6g and 6h lab reports

Quiz on Ch 7.1 Spectrophotometer

HW:

Read Ch 7.2 and do review questions

Do B.O pg 203

Finish Labs 7a and 7b – How to use a spectrophotometer – due on Thur 3/23

Day 2 Thursday, 3/23

In class:

Virtual Simulation on Buffer/pH

Review Ch 7.5 Use of Spectrophotometer to measure DNA Concentration

Finish Biotech Live 7.1 and 7.2 pgs 216-217

Read Biotethics pg 217-218: Test Results: who should have access to them?

HW:

Finish Lab 7a and 7b Lab reports due on Tues 3/28

Read Ch 7.3 Buffers and do review questions

Do B.O. pg 208

Finish Biotech Live 7.1 and 7.2 pgs 216-217

Read Biotethics pg 217-218: Test Results: who should have access to them?

Lab Activity: pH Lab next Tuesday

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 03/13/2023Date Due: 03/13/2023

HW:

Read Ch 7.4 and 7.5 Use of Spectrophotometers to measure Protein Concentration

Do 7.4 and 7.5 Review questions

Finish Lab 7a and 7b lab reports – due on Tues 3/21

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 03/06/2023Date Due: 03/06/2023

M/W  P1, P3, P5, P7

T/Th P2, P4, P6, P7

Fri P1-P7  (minimum day)

Day 1 Tuesday, 3/7

In class:

Review Lab 6g and 6 h Isolate HRP and Test HRP Activity using TMB

Review Ch 7.1 Spectrophotometer

Lab Activity: Lab 7a and 7b How to Use a Spectrophotometer

HW:

Finish Lab 7a and 7b How to Use a Spectrophotometer

Read Ch 7.4 Use of Spectrophotometers to measure Protein Concentration

Do 7.4 Review questions

Finish Lab 6g and 6h lab reports – due on Thurs 3/9

Finish Lab 7a and 7b lab reports – due on Tues 3/21

Day 2 Thursday, 3/9

In class:

Review Labs 7a and 7b How to use a spectrophotometer

Lab Activity: Soda Lab using the Spectrophotometer; create excel file, convert %T to A

Due today: Lab 6g and 6h lab reports

Quiz on Ch 7.1 Spectrophotometer

HW:

Read Ch 7.4 and 7.5 Use of Spectrophotometers to measure Protein Concentration

Do 7.4 and 7.5 Review questions

Finish Lab 7a and 7b lab reports – due on Tues 3/21

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 02/27/2023Date Due: 02/27/2023

M P1-P7 (Banking Day)

T/Th  P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 2/28

In class:

Read Ch 7.1 Spectrophotometer and do review questions pgs 195-197

Do B.O. 7.1 FTIR, pg 200

Read Ch 7.2 and do review questions pgs 200-203

Do B.O. pg 203 Learn how a pH meter electrode works

HW:

Edpuzzle: Spectrophotometer (in the library from 3:30 – 4:30 pm)

Finish Ch 7.1 and 7.2 review questions and B.O. 200 and 203

Read Lab 6h Testing for HRP with TMB – lab on Thurs 3/2

Finish Lab 6d Looking for a new product report – due on Thurs 3/2

Day 2 Thursday, 3/2

In class:

Group presentation on Ch 7. 1 Spectrophotometer

Lab Activity: Lab 6h Testing for HRP with TMB

Due today: Lab 6d Looking for a new product report

HW:

Finish Lab 6h Testing for HRP with TMB – due Thurs 3/9

Read Ch 7.3 Buffers and do review questions

Do B.O. pg 208 How and why proteins are purified from cells

Read Lab 7a and 7b How to Use a Spectrophotometer

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 02/20/2023Date Due: 02/20/2023

T/Th  P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 2/21

In class:

Review Ch 6.4 Western Blot and Ch 6.5 Looking for new products

Group Presentation on Ch 6.6 Producing rDNA and correct review questions

Lab Activity -Lab 6d Looking for new product, part 2 – test extract against E.coli

HW:

Finish Biotech Online pg 183 Quality Not and Quantity

Finish Biotech Live Activity 6.1-6.4 – due on Thurs 2/23

Ch 6 Test – Thurs 2/23; Ch 6 packet with review questions, B.O., Biotech Live -  due on Thurs 2/23

Day 2 Thursday, 2/23

In class:

Review Ch 6.6 Producing rDNA

Ch 6 Test

Due today: Ch 6 Packet with review questions, B.O., Biotech Live

Lab Activity: Finish Lab 6d - look for anti-microbial activity – due on Thurs 3/2

Read Lab 6g and 6h Isolation and Testing for HRP with TMB Lab – lab on Tues 2/28

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 02/13/2023Date Due: 02/13/2023

T/Th  P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 2/14

In class:

Stem Cell Lecture # 5 by Dr. Lu – last day

Stem Cell Debate

HW:

Group presentation on Thurs, 2/16 – 6.4 Western Blot and Ch 6.5 Looking for new products

Do Biotech Online pg 178 Amazon Hide and Seek

Read Lab 6d Looking for new product – lab on Thurs, 2/23

Bring your herbs

Day 2 Thursday, 2/16

In class:

Group presentation – Ch 6.4 Western Blot and Ch 6.5 Looking for new products

Lab Activity -Lab 6d Looking for new product, part 1

HW:

Read Ch 6.6 Producing rDNA and do review questions

Do Biotech Online pg 183 Quality Not and Quantity

Do Biotech Live Activity 6.1-6.4 – due on Thurs 2/23

Group Presentation – Ch 6.6 Producing rDNA and correct review questions – on Tues, 2/21

Ch 6 Test – Thurs 2/23; Ch 6 review questions-  due on Thurs 2/23

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 02/06/2023Date Due: 02/06/2023

M P1-P7  Banking Day

T/Th  P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 2/7

In class:

Stem Cells – Lecture #3 by Dr. Lu

HW:

Stem Cell #3 Blog

Read Ch 6.6 Producing rDNA and do review questions

Do Biotech Online pg 163 Quality Not and Quantity

Day 2 Thursday 2/9

In class:

Stem Cells – Lecture #4

HW:

Stem Cells #4 Blog

Do Biotech Live Activity 6.1-6.4 – due on Thurs 2/14

Group Presentation – Ch 6.6 Producing rDNA and correct review questions (will do on 2/11)

Ch 6 Test – Thurs 2/9; Ch 6 review questions-  due on Thurs 2/16

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 01/30/2023Date Due: 01/30/2023

M P1-P7  Banking Day

T/Th  P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 1/31 (PCC Stem Cell Module Starts today)

In class:

Review Ch 6.3 and go over ELISA Lab

Group presentation – 6.4 Western Blot, Ch 6.5Looking for new products

Due today: Lab 6c Amylase Lab

Lab Activity – Lab 6d Looking for new products, continue on Thurs

HW:

Read Ch 6.6 Producing rDNA and do review questions

Finish ELISA Anti-Heparinv Lab – due on Thurs 2/2

Day 2 Thursday 2/2

In class:

Review Lab 6e Looking for new products

Due today: ELISA Anti-Heparin Lab

Group Presentation – Ch 6.6 Producing rDNA and correct review questions

Lab Activity: continue Lab 6d part 2 - Test plant extract against E. coli

HW:

Do Biotech Live Activity 6.1-6.4 – due on Thurs 2/9

Lab 6d Looking for new products – due on Thurs 2/9

Ch 6 Test – Thurs 2/9; Ch 6 review questions-  due on Thurs 2/9

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 01/23/2023Date Due: 01/23/2023

M P1-P7  Banking Day

T/Th  P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 1/24

In class:

Review Ch 6.3 ELISA and Amylase

Finish Lab 6c Amylase Lab

Due today: Lab 6b Assay for Starch and Sugar

HW:

Read ELISA Lab – Lab on Thursday

Read Ch 6.4 Western Blots and do review questions

Day 2 Thursday, 1/26

In class:

Review Lab 6c Amylase

Group Presentation – Ch 6.4 Western Blot

Lab Activity: Anti-Heparin ELISA Assay

HW:

Read Ch 6.5 Looking for new products

Do B.O. pg 178 Amazon Hide and Seek

Lab 6c Assaying for Amylase Activity – due Tues 2/1

Continue working on Synthetic Biology Project

Bring Herbs for Tuesday and read Lab 6d Looking for Potential Medicine

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp 6 – Ch 7.2, 7.3

Grp 7 – Ch 8.1-8.5

Grp 8 – Ch 9.1-9.5

Grp 1,2,3 – Ch 12

Grp 6,7,8 – Ch 13

Date Assigned: 01/16/2023Date Due: 01/16/2023

T/Th  P1, P3, P5, P7

W/F  P2, P4, P6, P7

Day 1 Tuesday, 1/17

In class:

Review Ch 6.1 Potential Biotech Product and Ch 6.2 The use of Assays, correct review questions

Lab Activity: Lab 6b Assay for Starch and Sugar – due Tue 1/24

HW:

Read Ch 6.3 ELISA and do review questions 6.3

Do Biotech Online ELISA Technology in Diagnostic Kits pg 173

Due on Thurs, 1/19 – Rapid ELISA, TRU RSV

Day 2 Thursday, 1/19

In class:

Review Ch 6.2 Assays

Group 1 Presentation - Ch 6.3 ELISA, correct review questions

Due today: Rapid ELISA, TRU RSV

Lab Activity: Lab 6c Assaying for Amylase Activity

HW:

Read ELISA Lab

Finish Lab 6c Amylase Activity – due Thurs 1/26

Edpuzzle – ELISA

Continue working on Synthetic Biology Project

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp 6 – Ch 7.2, 7.3

Grp 7 – Ch 8.1-8.5

Grp 8 – Ch 9.1-9.5

Grp 1,2,3 – Ch 12

Grp 6,7,8 – Ch 13

Date Assigned: 01/09/2023Date Due: 01/09/2023

M P1-P7 Banking Day

T/Th  P1, P3, P5, P7

W/F  P2, P4, P6, P7

Day 1 Tuesday, Jan 10

In class:

Review Ch 5 Proteins and PAGE, Polyacrylamide Gel Electrophoresis

Go over Synthetic Biology Project –due in 10 weeks

Assign chapters for group presentations

HW:

Read Ch 6.1 Assays

Do review questions 6.1

Brainstorm ideas for Synthetic Biology Project

Click here: https://biobuilder.org/lab/biology-by-design/?a=student

Day 2 Thursday, Jan 12

In class:

Go over Synthetic Biology – go to www.Biobuilder.org

Ch 6.1 Potential Products

Lab Activity: Rapid ELISA antibody assay

HW:

Read Ch 6.2 Assays

Do review questions 6.2

Rapid ELISA antibody Assay – Lab report due on Thurs, Jan 19

Brainstorm ideas for Synthetic Biology Project

Click here: https://biobuilder.org/lab/biology-by-design/?a=student

Chapter Assignments: Please prepare a Powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp 6 – Ch 7.2, 7.3

Grp 7 – Ch 8.1-8.5

Grp 8 – Ch 9.1-9.5

Grp 1,2,3 – Ch 12

Grp 6,7,8 – Ch 13

Date Assigned: 12/12/2022Date Due: 12/12/2022

Monday P1– P7 regular schedule

T/Th P1, P3, P5, P7

W/F P2, P4, P6, - Final exam P7

Day 1 Tuesday, 12/13

In class:

Due today: Ch 5 Packet

Hands on Activity: Constructing a paper recombinant plasmid

Review for Finals

HW:

Study for Finals, Units 1-4 – Finals on Tuesday

Finish Study Guide for Finals

Day 2 Thursday Dec 15

Final Exam – Chapters 1-5

Good luck on the rest of your finals!

Date Assigned: 12/05/2022Date Due: 12/05/2022

M –  Banking Day, P1-P7

T/Th P1, P3, P5, P7

W/F  P2, P4, P6, P7

Tuesday, Dec 6

In class:

Due today -– Antibody-Antigen: HCG and iFOBT (Occult Blood) Lab reports

Go over Graphing Data from Catalase Lab – Lab report due Tues 12/13

Correct Ch 5.3-5.4 Protein Analysis

Lab Activity: PAGE – Polyacrylamide Gel Electrophoresis simulation: biomodel.uah.es/en/lab/SDS-PAGE/inicio.htm

Homework:

Study for Ch 5 Test – Test on Thurs 12/9

Read Ch 5.5 Applications of Protein Analysis

Read Ch 5 Summary and Lab Practices pgs 158-159

Finish Catalase Lab – due Tues, 12/13

Thursday, Dec 8

In class:

Go over PAGE – Polyacrylamide Gel Electrophoresis

Do Biotech Live Activity 5.1-5.3 and correct Ch 5.4 Review Questions

Ch 5 Test

Homework:

Complete Ch 5 Packet – due Tues 12/13

Finish Catalase Lab – due Tues, 12/13

Finish PAGE Lab report – due Thurs 12/15

Study for Finals, Ch 1-5 – Finals on Thurs 12/15

Date Assigned: 11/28/2022Date Due: 11/28/2022

M –  Banking Day, P1-P7

T/Th P1, P3, P5, P7

W/F  P2, P4, P6, P7

Tuesday, Nov 29

In class:

Review Ch 5.1-5.3 Protein Enzymes and correct assignments

Due today -– ABE Lab 7 Isolation of RFP by Column Chromatography

Lab Activity – Antibody-Antigen Test

Homework:

Read Ch 5.1-5.3 more on Proteins

Complete Antibody-Antigen Test lab report – due Tues, 12/6

Thursday, Dec 1

In class:

Review Enzymes and Studying Proteins

Lab Activity – Catalase Lab, parts A-D

Homework:

Graph results of Catalase Lab

Read PAGE Lab (Polyacrylamide Gel Electrophoresis)

Review Ch 5.4 and Study for Ch 5 Test

Ch 5 Test – Thurs, 12/8

Date Assigned: 11/21/2022Date Due: 11/21/2022

Reminder:  Finish ABE Lab 7 Isolation of RFP by Column Chromatography – due on Tue 11/29

Read Ch 5.4 Studying Proteins and Ch 5.5 Analyzing Proteins and do review questions

B.O. Protein Sequencers – pg 156

Happy Thanksgiving!

Date Assigned: 11/14/2022Date Due: 11/14/2022

M –  Banking Day, P1-P7

T/Th P1, P3, P5, P7

W/F  P2, P4, P6, P7

Tuesday, Nov 15

In class:

Presentations on Genetic Disorders from NCBI/BLAST activity

Ch 5.1 and 5.2 Protein Structure and Function

Lab Activity today – ABE Lab 7 Isolations of RFP by Column Chromatograpy; do prelab questions

Quiz on Ch 5.1 Proteins

Homework:

Read Ch 5.3 Protein Catalysts and do review questions

B.O. Catalysts for Better Health pg 151

Read ABE Lab 7 Overnight Cultures of Transformed E.coli; do prelab questions

Read ABE Lab 7 Isolations of RFP by Column Chromatography

ABE Lab 6 Overnight Culture of transformed E.coli cells – due on Thurs 11/17

Thursday, Nov 17

In class:

Go over ABE Lab 7 Isolations of RFP by Column Chromatography

Ch 5.2 and 5.3 Protein Production and Protein Activity; correct review questions

Finish Genetic Disorder presentations

Due today: ABE Lab 6 O/N Culture of transformed E.coli cells

Homework:

Finish ABE Lab 7 Isolation of RFP by Column Chromatography – due on Tue 11/29

Read Ch 5.4 Studying Proteins and Ch 5.5 Analyzing Proteins and do review questions

B.O. Protein Sequencers – pg 156

HAPPY THANKSGIVING!

Date Assigned: 11/07/2022Date Due: 11/07/2022

M/W  P1, P3, P5, P7

T/Th  P2, P4, P6, P7

Friday – Veteran's Day, No School

Tuesday, Nov 8

In class:

Review ABE Lab 4 and 5 – Confirmation of Digest and Transformation

Lab Activity today – ABE Lab 6 Set up Overnight Culture of Transformed Cells; Screen for Transformed, red cells

Quiz on ABE Lab 4, 5 Confirmation, and Transformation

Homework:

ABE ABE Lab 4, 5 Confirmation, Transformation due Thursday, 11/10

Read Ch 5.1 Protein structure and function and do review questions

B.O. Antibody Producing Companies

Read ABE Lab 6 Overnight Cultures of Transformed E.coli; do prelab questions

Thursday, Nov 10

In class:

Go over ABE Lab 6 Overnight Culture

Lab Activity: ABE Lab 6 Set up Overnight Culture.

Ch 5.2 Protein Production and Protein Activity

Due today: ABE Lab 4 Confirmation of Restriction Digest

Homework:

Read ABE Lab 7 Isolation of RFP by Column Chromatography

Read Ch 5.2 Protein Production

B.O. Couch Potatoes pg 146

Date Assigned: 10/31/2022Date Due: 10/31/2022

M/W  P1, P3, P5, P7

T/Th  P2, P4, P6, P7

F P1-P7 Shakeout Assembly Schedule

Tuesday, Nov 1

In class:

Review ABE Lab 4 – Confirmation of Restriction Digest of pAra and pKan-R and Ligation

Hands-on Activity – HASPI Diagnosing Genetic Disease Through Gel Electrophoresis

ABE Labs 2 and 3 lab reports due on Thursday, 11/4

Quiz on ABE Labs 1 -3

Homework:

Read ABE Lab 5 Transformation of E.coli with pAra-R

Finish Activity on Diagnosing Genetic Disease Through Gel Electrophoresis – Due on Tue 11/8

Complete ABE Labs 2 and 3 Ligation – Due on Thurs 11/4

ABE Lab 4 Confirmation – Due Tue 11/8

Finish Ch 4 Packet – due Thurs 11/4

Thursday, Nov 3

In class:

Go over Bacterial Transformation

Lab Activity: ABE Lab 5 Transformation of E.coli with pAra-R

Activity: Bioinformatics NCBI and Blast

Due today: ABE Labs 2 and 3 lab reports and Ch 4 packet

Quiz on Restriction digest and Gel Electrophoresis

Homework:

Read ABE Lab 6 Overnight Culture of Transformed E.coli

Finish ABE Labs 4 report – due Thurs 11/11

Finish Activity on Diagnosing Genetic Disease Through Gel Electrophoresis – Due on Tue 11/8

Finish : Bioinformatics NCBI and Blast – due on Tues, Nov 8

Date Assigned: 10/24/2022Date Due: 10/24/2022

M/W  P1, P3, P5, P7

T/Th  P2, P4, P6, P7

Tuesday, Oct 25

In class:

Go over Gel Electrophoresis

Correct 4.4 review questions; Ch 4 Test on Thursday, 10/27

Set up ABE Lab 3 Ligation of pAra and pKan

Do/Finish CTE Modules 2 & 3

Due today: ABE Lab 1 Gel Electrophoresis

Homework:

Read ABE Lab 3 Ligation and Lab 4 Confirmation of pAra and pKan

Finish Biotech Live 4.1-4.6

Study for Ch 4 Test – Test on Thursday, 10/27

Thursday, Oct 27

In class:

Ch 4 Test

Go over ABE Lab 3 Ligation and Lab 4 Confirmation of pAra and pKan

Lab 4: Lab 4 Confirmation of pAra and pKan by Gel Electrophoresis

Homework:

Due: ABE Lab 4 Confirmation of Restriction Digest by Gel Electrophoresis

Finish ABE Labs 2 and 3 report – due Thursday, 11/3

Finish Ch 4 Packet – Review Questions Ch 4.1-4.4, Biotech Online 4.1-4.4, Biotech Live 4.1-4.6 – due on Thursday, 11/3

Date Assigned: 10/17/2022Date Due: 10/17/2022

M P1-P7 Banking Day

T/Th P1, P3, P5, P7

W/F  P2, P4, P6, P7

Tuesday, Oct 18

In class:

Go over 4.3 DNA Structure and Isolation of DNA

Quiz on Ch 4.1-4.3

Lab Activity: ABE Lab 1 Gel Electrophoresis

Due: Strawberry DNA Isolation Lab

Homework:

Read Ch 4.4 Gel Electrophoresis and do review Questions

Finish ABE Lab 1 Prelab Notes

Do B.O. Chop and Go Electrophoresis pg 124

Do Ch 4 Biotech Live 4.1-4.2

ABE Lab 1 Gel Electrophoresis – due on Tuesday 10/26

Thursday, Oct 20

In class:

Go over Ch 4.4 Gel Electrophoresis

Quiz on Gel Electrophoresis

Homework:

Do Biotech Online Chop and Go pg 124

Finish ABE Lab 1 Gel Electrophoresis – due on Tuesday 10/26

Read ABE Lab 2 Restriction Digest of pKan and pARA

Read Ch 4 Summary and Lab Practices pgs 126-127

Do Ch 4 Biotech Live 4.4-4.6

Ch 4 Test on Thursday, 10/28

Date Assigned: 10/10/2022Date Due: 10/10/2022

M  P1, P3, P5, P7

T/Th P2, P4, P6, P7

W PSAT 8:30 am - 12:57 pm

F P1-P7 Minimum Day

Tuesday, Oct 11

In class:

Ch 4.1 DNA Structure

Lab Activity: Isolation of Strawberry DNA

Due today: Ch 3 Packet and Math Problems; packet should include review questions, Biotech Online, and Biotech Live

Homework:

Read Ch 4.2 Sources of DNA, do review questions

Do Biotech Online Know your Genome pg 112, Baldness Gene pg 118

Download Amgen Lab Manual

Strawberry DNA Lab – due on Tuesday, 10/18

Thursday, Oct 13

In class:

3 pm: Special Guest: Ms. Samantha Male – presentation on her own project on Stem Cells

Ch 4.2 Sources of DNA, correct review questions

Activity: Cast a Gel for Gel Electrophoresis; make 1x SB buffer and 1% agarose gel

Homework:

Read Ch 4.3 Isolating and Manipulating DNA

Do Biotech Online Recombinant Pharmaceuticals and Therapies, pgs 118-119

Read Lab 1 Amgen Lab Manual

Strawberry DNA Lab – due on Tuesday, 10/18

Date Assigned: 10/03/2022Date Due: 10/03/2022

M P1-P7 (Banking Day)

T/Th  P1, P3, P5, P7

W/F  P2, P4, P6, P7

Tuesday, Oct 4

In class:

Correct Ch 3.6 review questions and Math Problems

Ch 3 Test on Thursday

Due today: Pipetting Accuracy Lab, parts 1 and 2

Classwork: Biotech Live – Activity 3.1-3.4 pgs 95-97 (work on Chromebook)

Homework:

Study for Ch 3 Test – Test on Thursday

Finish Biotech Live – Activity 3.1-3.4 pgs 95-97 and finish Math problems

Micro-pipetting Lab with colored dyes – due Thurs, 10/6

Thursday, Oct 6

In class:

Ch 3 Test

Continue with Biotech Live – Activity 3.5-3.6 pgs 98-100

Go over Math/MW of compounds

Activity: more practice on micro-pipetting

Homework:

Read Ch 4.1 Studying DNA and do review questions

Read Lab 1 Amgen Lab Manual

Bring Strawberries for Tuesday

Date Assigned: 09/19/2022
Date Due: 09/19/2022
M P1-P7 (Banking Day)

T/Th  P1, P3, P5, P7

W/F  P2, P4, P6, P7

Tuesday, Sept 20

In class:

Correct all Ch 2.4 and 2.5 review questions

Go over Bacteria Lab and Gram Stain

Lab Activity – Gram Stain and use microscope to view bacteria

Homework:

Ch 2 Test on Thursday and Ch 2 packet due on Thursday

Read Ch 3.1-3.2 pgs 71-82 and do review questions

Do B.O. pg 74 on Metrics Conversion

Finish Bacteria Lab– due on Tuesday, 9/27

Microscope Lab – due on Thursday, 9/22

Thursday, Sept 22

In class:

Ch 2 Test and Ch 2 packet due today

Go over Ch 3.1-3.2 review questions and math problems

Go over Gram Stain Lab

Lab Activity – Pipetting Accuracy

Due today: Microscope Lab

Homework:

Read Ch 3.3-3.4 and do review questions

Do Math Problems

Grams Stain Lab – due on Thurs, 9/29

Pipetting Lab due Thurs, 9/29

Date Assigned: 09/12/2022Date Due: 09/12/2022

M         P1-P7 Banking Day

T/Th    P1, P3, P5, P7

W/F     P2, P4, P6, P7

Tuesday, Sept 13

In class:

Go over Organic Lab and complete Microscope Lab

Correct Ch 2.1-2.3 review questions

Due today: Cheese Lab

Lab Activity – Bacteria Lab: collect bacteria, streak media plate, test against antibiotics

Homework:

Read Ch 2.4 New Biotechnology and do 2.4 review questions

Do B.O. pgs 43 Kimchi, 46 Right Tool, 52 Molecular Models, and 82 Biotech Products

Finish Cheese Lab with Chymax and Organic Lab -  both are due on Thursday, 9/15

Microscope Lab – due on Thursday, 9/22

Ch 2 Test on Thursday, 9/22

Thursday, Sept 15

In class:

Due today: Cheese Lab with Chymax and Organic Lab

Correct Ch 2.4 review questions

Go over Bacteria Lab and Gram Stain

Lab Activity: Gram Staining of bacteria

Homework:

Finish Bacteria Lab – due on Thursday, 9/22

Do Biotech Live Activity 2.1-2.3 pg 86

Read Ch 2 Summary and Lab Practices pg 81-86

Ch 2 Test on Thursday, 9/22

Date Assigned: 09/05/2022Date Due: 09/05/2022

T/Th  P1, P3, P5, P7

W P2, P4, P6, P7

F P1-P7 (Minimum Day)

Tuesday, Sept 6

In class:

Review Ch 1.3-1.6 and correct review answers

Discuss careers and Biotethics in Biotechnology- watch video on Biochemist

Check cheeseball – make final observation: color, texture, smell

Lab Activity: Organic Lab – lab report due on

Homework:

Read Summary and Lab Practices on pgs 30-31 to review for Ch 1 Test

Study for Ch 1 Test – Test on Thursday, Sept 8

Cheese Lab is due on Tuesday, Sept. 13

Cheese Lab with Chymax – due on Thursday, Sept 15

Read Ch 2.1, do review question and Label parts of Microscope (worksheet) due on Thursday 9/8

Remember to copy review questions and answer in complete sentences on a separate sheet

Thursday, Sept 8

In class:

Ch 1 Test in class on Quizziz

Go over Ch 2.1 Review Questions, Organic Lab, and Microscope worksheet

Lab Activity: Microscope Lab and do cells worksheet on structures and function

Homework:

Read Ch 2.2-2.3, do review questions; remember to copy questions and answer in complete sentences

Complete cell structure and function worksheet

Finish Cheese Lab – due on Tues, 9/13

Cheese Lab with Chymax – due on Thurs, Sept 15

Finish Organic Lab – due on Thurs, 9/15

Date Assigned: 08/29/2022Date Due: 08/29/2022

M     -   P1-P7 (Banking Day)

T/Th - P1, P3, P5, P7

W/F - P2, P4, P6, P7

Tuesday, August 30

In Class:

What is Scientific Method?

Correct Ch 1.2-1.3 review questions

Lab Activity: Cheese Lab, make first observation, record in your notebook

Biotech Live:  Activity 1 What is Biotechnology? pgs 32-33

Activity 2 Business Side pgs 33-34

Bleach Lab due today– Did you send to intern to check for content/format?

HW:

Read ED Ch. 1.4 and review questions pgs 19-23 ED

Do B.O. Hot Jobs pg 26

Extra Credit: make cheese at home; DIY on foodwishes.com

Thursday, Sept 1

In Class:

Cheese Lab - Make ^2nd observation, record in your notebook ready for cheese cloth?

Discuss Careers in Biotech

Do Biotech Live Activity 1.4 How Biotech Industry Improving Human Life? pg 35

Correct Ch 1.4 review questions

HW:

Read ED Ch 1.5-1.6 and do review questions pgs 23-29 ED

Do B.O. Code of Business Conduct pg 29 ED

Read Summary and Lab Practices pgs 30-31

Please share your lab report with your intern to get it checked before turning it in to me.

Biotech Interns: Here are your interns:
Kayleen Yang kyan2348@stu.gusd.net                   A-Cabrera
Rosalia Park - rpar7075@stu.gusd.net                      

Rachel Lee rlee5733@stu.gusd.net                         Chang to H

Claire Yang - cyan6089@stu.gusd.net
Nate Jaramillo njar6348@stu.gusd.net J to L

Michelle Chung - mchu2590@stu.gusd.net
Milena and Astgik  mjag6984@stu.gusd.net         P-V  asha9484@stu.gusd.net

Nathan Pang - npan1489@stu.gusd.net

These are our presidents and will help if your intern is too busy. So don't hesitate to send it to them.

Rosalia Park - rpar7075@stu.gusd.net       Nate Jaramillo njar6348@stu.gusd.net                
Claire Yang - cyan6089@stu.gusd.net    Rachel Lee rlee5733@stu.gusd.net                   
Michelle Chung - mchu2590@stu.gusd.net              
Nathan Pang - npan1489@stu.gusd.net           

Date Assigned: 08/22/2022Date Due: 08/22/2022

T/Ths – 2:40-4:50 pm

M     -   P1-P7 (Banking Day)

T/Th - P1, P3, P5, P7

W/F - P2, P4, P6, P7

Tuesday, Aug 23

In class:

What is Biotech?

Go over How to maintain a Scientific Notebook in Biotech

Lab Activity: Bleach Lab pgs 19-21 TB

HW:

Read Ch 1.1-1.2 and do review questions Ch 1.1-1.2 in ED - write questions and answers on paper with black ink

Read Cheese Lab before Thursday and bring whole milk and buttermilk on Thursday

Do Biotech Online, B.O., pg 13 Glo Fish

Day 2 Thursday, Aug 25

In class:

Complete and make final observations for Bleach Lab, lab report due on Th 9/1

Review How to Notebook and how to write an effective conclusion

Lab Activity: Cheese Lab

HW:

Read Ch 1.3 pgs 14-18 and do review questions 1.3 ED

Do Biotech Online (B.O.) pg 18 Genentech

Finish Bleach Lab report in Google Docs pgs 19-21 ED; lab report due on Th 9/1

Date Assigned: 08/15/2022Date Due: 08/15/2022

Day 1 Wednesday, August 17

In class:

Rules and Policies

Safety Rules

HW:

Sign up for Google Classroom, Quizizz

Study for Safety Test – Test on Thursday

Wear shoes to class – Always!

Day 2 Thursday, August 18

In class:

Safety Test

What is Biotechnology

HW:

Read Ch 1.1 Biotechnology ED (Ellyn Daugherty textbook)

Read Bleach Lab pgs 19-21 ED, Lab on Tuesday

Wear shoes, = please

Date Assigned: 06/06/2022Date Due: 06/06/2022

M P1, P3, P5, P7

T P2, P4, P6, P7, Senior Finals

W P1, P3, P5, P7

Th P1- P6, Finals in P7 

F P1, P3, P5

Day 1 Tuesday, 6/7

In class:

Lab Activity: DB Lab 6 Spin Column to purify CO1 Amplicon 

Group Presentation on Ch 13.3-13.4 Applications of PCR

HW: 

Read DB Lab 8 Gel Electrophoresis to confirm CO1 Amplicons

Study for Finals, Ch 6-9, 12, 13 – Finals on Thursday, 6/9

Day 2 Thursday 6/9

In class:

Lab Activity: DB Lab 8 Gel Electrophoresis to confirm CO1 Amplicons

Finals today! - Ch 6-9, 12, 13

Date Assigned: 05/30/2022Date Due: 05/30/2022

Monday – Memorial Day Holiday – No School

T/Th P1, P3, P5, P7 

W/F P2, P4, P6, P7 

Day 1 Tuesday, 5/31

In class:

Lab Activity: DB Lab 4 Gel Electrophoresis 

Go over DB Lab 5 Amplifying CO1 by PCR, Polymerase Chain Reaction

Group Presentation on Ch 12.5 Recent Advances

HW: 

Read DB Lab 6 Spin Column to Isolate CO1 Amplicons

Read Ch 13.1-13.2 Making DNA Molecules, do review questions

Study for Finals – Finals on Friday, 6/10

Day 2 Thursday 6/2

In class:

Lab Activity: DB Lab 6 Spin Column to Isolate CO1 Amplicons

Group Presentation on Ch 13.3-13.5 Polymerase Chain Reaction

HW: 

Read DB Lab 7 Confirmation of CO1 Amplicons

Study for Finals – Finals on Friday, 6/10

Date Assigned: 05/23/2022Date Due: 05/23/2022

M/Th P1, P3, P5, P7 

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 5/24

In class:

Group Presentation – Ch 12.4 Pharmaceuticals

Lab Activity: DB Lab 2 Isolating Genomic DNA and Lab 2 Casting Gel 

HW: 

Read DB Lab 3 Casting Gel and DB Lab 4 Gel Electrophoresis

Do B.O. Getting Sick pg 354

Do B.O. Eat Veggies pg 355

Day 2 Thursday 5/26

In class:

Lab Activity: DB Lab 4 Gel Electrophoresis

HW: 

Read DB Lab 5 and Lab 6 Amplifying CO1 and Confirmation

Read Ch 12.5 Recent Advances in Medical Biotechnologies

Do B.O. Medicine for you pg 357

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 05/16/2022Date Due: 05/16/2022

M/Th P1, P3, P5, P7 

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 5/17

In class:

Group Presentation - Ch 9.5 Marketing and Sales

Group Presentation – Ch 12.1 Drug Discoveries

DNA Barcoding

HW: 

Read Ch 12.2 Creating Pharmaceuticals and do review questions

Read Intro to DNA Barcoding: DB Lab 1 and Lab 2 – Isolate DNA from Bees

Day 2 Thursday 5/19

In class:

Group Presentation - Ch 12.2 Creating Pharmaceuticals

Lab Activity: DB Lab 2 Isolate DNA from Bees

HW: 

Read DB Lab 3 Casting Gel and DB Lab 4 Gel Electrophoresis

Read Ch 12.3 Creating Pharmaceuticals through Peptide Synthesis and do review questions

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 05/09/2022Date Due: 05/09/2022

M/Th P1, P3, P5, P7 

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 5/10

In class:

Check Protein Concentration of isolated RFP

Review Column Chromatography 

Column Chromatography to isolate RFP lab report – due on today

Group Presentation: Ch 9.4 Product Quality – correct HW

Lab Activity: Check protein concentration of isolated RFP

Ch 9 Test on Thursday

HW: 

Review Ch 9.5 and finish Ch 9.5 review questions

Read Ch 9 Summary and Lab Practices pgs 274-275

Do Biotech Live: Activity 9.1 Protein Manufacturing and 9.2 Setting Standard

Day 2 Thursday 5/12

In class:

Group Presentation: Ch 9.5 Marketing and Sales and correct review questions

Do Biotech Live: Activity 9.3 Reading Annual Reports

Read Bioethics Part I pg 278 and Part II pg 27

Ch 9 Test – Today, Thursday

HW: 

Ch 9 Packet – due on Tuesday 5/17

Read Ch 12.1-12.2 Drug Discoveries pg 343-348 and do review questions

B.O. pg 344 New Medicines

B.O. pg 345 Penicillin

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 05/02/2022Date Due: 05/02/2022

M/Th P1, P3, P5, P7 

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 5/3

In class:

Due today: Mini Prep Lab Report

Review Mini prep, check for DNA

Lab Activity: Column Chromatography to isolate RFP

HW: 

Review Ch 9.3 more on Column Chromatography and finish 9.3 review questions

Do Ch 9.3 B.O. pg 268 Got Gas

Read Ch 9.4 Product Quality and do review questions

Do Ch 9.4 B.O. pg 279 Products in the Pipeline 

Day 2 Thursday 5/5

In class:

Group Presentation: Ch 9.3 more on Column Chromatography

Group Presentation: Ch 9.4 Product Quality

Check Protein Concentration of isolated RFP

Quiz on Ch 9.3-9.4 today

HW: 

Read Ch 9.5 Marketing and Sales and do review questions

Do Ch 9.5 B.O. Biotech Drugs pg 273

Column Chromatography lab report – due on Tues 5/10

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 04/25/2022Date Due: 04/25/2022

M/Th P1, P3, P5, P7 

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 4/26

In class:

Review Mini/Maxi Prep

Group presentation – Ch 9.1 Harvesting a Protein Product

Lab Activity: Mini Prep using BioRad kit; also set up an overnight culture for column chromatography

HW: 

Read Ch 9.2 Column Chromatograpy and do review questions pgs 253-258

EP: Column Chromatography – due on Thurs 4/28 @ 8 am

Day 2 Thursday 4/28

In class:

Review Ch 9.1 and correct review questions

Group presentation Ch 9.2 Column Chromatography

Lab Activity: Column Chromatography to isolate a protein

HW: 

Read Ch 9.3 more on Column Chromatography

EP: HPLC due Tues 5/4

Mini-Prep lab report – due on Tues 5/4

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 04/18/2022Date Due: 04/18/2022

M/Th P1, P3, P5, P7 

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 4/19

In class:

Go over Karyotype

Stem Cell Module #5 by Dr. Karol Lu – Day #5 @ 2:45 pm

Final Activity: Stem Cell Debate

HW: 

Study for Ch 8 Test – Test on Tues 4/20

Read Ch 8 Summary and Lab Practices pgs 246-248

Do Thinking Like a Biotechnician pg 248

Do Biotech Live Activity – 8.1-8.5

Ch 8 packet due on Wednesday

Day 2 Wednesday 4/20

In class:

Group presentation Ch 8.5 and correct Ch 8.5 review questions

Ch 8 Test and Ch 8 packet due today

HW: 

Read Ch 9.1 Bringing product to market and do review questions

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 04/11/2022Date Due: 04/11/2022

M/Th P1, P3, P5, P7 

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 4/12

In class:

Correct Ch 8.5 Retrieving Plasmids and do review questions

Stem Cell Module #3 by Dr. Karol Lu – Day #3 @ 2:45 pm

HW: 

Stem Cells HW #3 – Study for Stem Cells Quiz

Study for Ch 8 Test – Test on Tues 4/19

Read Ch 8 Summary and Lab Practices pgs 246-248

Do Thinking Like a Biotechnician pg 248

Day 2 Thursday 4/14

In class:

Stem Cell Module #4 by Dr. Karol Lu – Day #3 @ 2:45 pm

HW: 

Stem Cells HW #3 – Study for Stem Cells Quiz

Study for Ch 8 Test – Test on Tues 4/19

Do Biotech Live Activity 8.1-8.4 pgs 248-250

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 04/04/2022Date Due: 04/04/2022

M/Th P1, P3, P5, P7 

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 4/5

In class:

Correct Ch 8.2-8.3 Transformation and After Transformation assignments 

Quiz on Ch 8.2-8.3 Transformation and After Transformation

Go over Lab 4 Confirmation of Restriction Digest

Stem Cell Module #1 by Dr. Karol Lu from PCC CIRM program

HW: 

Read Ch 8.5 Retrieving Plasmids and do review questions

Stem Cells HW #1

Ch 8 Test on Tues, 4/12

Day 2 Thursday 4/7

In class:

Review Ch 8.4 and 8.5 Fermentation and correct assignments

Stem Cell Module #2 by Dr. Karol Lu from PCC CIRM program

HW: 

Read Ch 8 Summary and Lab Practices pg 246-248

Do Biotech Live Activity 8.1-8.4 

Stem Cells HW #2

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 03/28/2022Date Due: 03/28/2022

M/Th P1, P3, P5, P7 

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 3/29

In class:

Go over ABE Labs 2 and 3 Restriction Digest and Ligation of pDrk and pGrn

Group presentation - Ch 8.2 Transformation

Do prelab notes ABE Lab 5

Lab Activity: ABE Lab 5 Transformation

Due today: Synthetic Biology Project, Group presentations begin 

HW: 

Review Ch 8.3 After Transformation

Finish ABE Labs 2 and 3 – due Thurs 3/31

Read Amgen (ABE) Manual green Labs 4 and do pre lab notes

Day 2 Thursday, 3/31

In class:

Due: ABE Labs 2 and 3  and go over ABE Lab 5 Transformation and screen for green glowing cells

Set up ABE green Lab 4 Confirmation of Digest

Group presentation: Ch 8.3 After Transformation

HW: 

Read Ch 8.4 After Transformation and do review questions

Finish ABE green Labs 4 and 5 Confirmation of Digest and Transformation lab report – due Thurs 4/7

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 03/21/2022Date Due: 03/21/2022

T/Th P1, P3, P5, P7 

W/F P2, P4, P6, P7 

Day 1 Tuesday, 3/22

In class:

Group presentation - Ch 8.1 Recombinant Biotechnology

Set up Restriction Digest of pDrk and pGrn

Due today: Synthetic Biology Project, Group presentations begin 

HW: 

Read Ch 8.2 Transforming Cells and do review questions

Do B.O. BACS and YACS pg 228

Read Amgen (ABE) Manual green Labs 2 and 3 Restriction Digest and Ligation of pDrk and pGrn

Day 2 Wednesday, 3/23

In class:

More group presentation Synthetic Biology Project 

Set up ABE green Lab 3 Ligation to construct rpGlo.

HW: 

Read Ch 8.3 After Transformation and do review questions

Read ABE green Labs 4 and 5 Confirmation of Digest and Transformation

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 03/14/2022Date Due: 03/14/2022

Just a friendly remember:

Finish Synthetic Biology Project – due on Tues 3/22

Read Ch 8.1 Production of Recombinant Biotech Prod and do review questions - due on Tues 3/22

Do B.O. Genetic Engineering pg 226 – due on Tues 3/22

Group Presentation on Ch 8.1 – on Tuesday 3/22

HAVE A GREAT SPRING BREAK!

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

HAVE A GREAT SPRING BREAK!

Date Assigned: 03/07/2022Date Due: 03/07/2022

M/W P1, P3, P5, P7

T/Th P2, P4, P6, P7 

Fri P2, P4, P6, P7 

Day 1 Tuesday, 3/8

In class:

Due today: pH Lab

Review Ch 7.2 and pH 

Group presentation Ch 7.3 Buffers correct 7.3 review questions

CW: Biotech Live: Do Activity 7.1-7.3 pg 216-277

HW: 

Review Ch 7.1-7.5

Read Ch 7 Summary and Lab Practices pgs 214-216

Finish Biotech Live: Do Activity 7.1-7.3 pg 216-277

Ch 7 Test on Thursday 3/10

Day 2 Thursday, 3/10

In class:

Ch 7 Test

Work on Synthetic Biology Project – due on Tues 3/22

HW: 

Finish Synthetic Biology Project – due on Tues 3/22

Read Ch 8.1 Production of Recombinant Biotech Prod and do review questions

Do B.O. Genetic Engineering pg 226

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 02/28/2022Date Due: 02/28/2022

M/Th P1, P3, P5, P7

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 3/1

In class:

Due today: Soda Lab

Group presentation: Ch 7.5 Use of Spectrophotometer to measure DNA Concentration

Activity: Virtual Simulation on Buffer/pH

HW: 

Read Ch 7.2 and do review questions

Do B.O. pg 203 Learn how a pH meter electrode works

Due on Tuesday, 3/8 – Virtual Simulation on Buffer/pH

Day 2 Thursday, 3/3

In class:

Review Ch 7.5 Use of Spectrophotometer to measure DNA Concentration

Group presentation on Ch 7. 2 Buffers/pH

Lab Activity: pH Lab

HW: 

Finish pH Lab report due on Tues 3/10

Read Ch 7.3 Buffers and do review questions 

Do B.O. pg 208 How and why proteins are purified from cells

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 02/21/2022Date Due: 02/21/2022

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7 

Day 1 Tuesday, 2/22

In class:

Due today: Lab 6g and 6h Isolation and Testing for HRP with TMB Lab report due today

Review Lab 7a and 7b How to Use a Spectrophotometer

Lab Activity: Soda Lab using the Spectrophotometer; create excel file, convert %T to A

Quiz on Ch 7.1 Spectrophotometer

HW: 

Finish Lab 7a and 7b How to Use a Spectrophotometer lab reports – due on Thurs 2/24

Soda Lab report due on Tues 3/1

Review Ch 7.4 and finish review questions

Day 2 Thursday, 2/24

In class:

Review Lab Activity: Soda Lab

Group presentation on Ch 7. 4 Using Spectrophotometer to find concentration of protein

Due today Lab Activities: Lab 7a and 7b – How to Use a Spectrophotometer

Lab Activity: Virtual Simulation on Using Spectrophotometer to measure concentration of molecules

HW: 

Finish Soda Lab report due on Tues 3/1

Read Ch 7.5 Use of Spectrophotometers to DNA and do 7.5 Review questions

Finish Virtual Simulaltion on Spectrophotometer – due on Tue 3/1

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 02/14/2022Date Due: 02/14/2022

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7 

Day 1 Tuesday, 2/15

In class:

Review Lab 6d Looking for new product; Lab report due today

Lab Activity 6g and 6 h Isolate HRP and Test HRP Activity using TMB

HW: 

Read Ch 7.1 Spectrophotometer and do review questions

Do B.O. 7.1 FTIR, pg 200

Lab 6g and 6h lab reports – due on Tue 2/22

Read Lab 7a and 7b How to Use a Spectrophotometer

Day 2 Thursday, 2/17

In class:

Review Lab Activity: Lab 6g Isolate HRP

Group presentation on Ch 7.1 Spectrophotometers

Lab Activities: Lab 7a and 7b – How to Use a Spectrophotometer

HW: 

Read Ch 7.4 Use of Spectrophotometers to measure Protein Concentration

Do 7.4 Review questions

Finish Lab 6g and 6h lab reports – due on Tue 2/22

Finish Lab 7a and 7b lab reports – due on Thurs 2/24

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 02/07/2022Date Due: 02/07/2022

M/Th P1, P3, P5, P7

W P1, P3, P5, P7 (Banking Day)

T/F P2, P4, P6, P7 

Day 1 Tuesday, 2/8

In class:

Review Ch 6.6 Production of recombinant protein

Lab Activity – Lab 6d Looking for new products, part 2, test extract against E.coli

HW: 

Read Ch 6 Summary pgs 184-185, due on Thurs 2/3

Do Lab Practices pgs 185, due on Thurs 2/3

Finish Biotech Live Activity 6.1-6.4 – due on Thurs 2/3

Ch 6 Review questions - due on Thurs 2/3

Ch 6 Test – Thurs 2/10

Day 2 Thursday, 2/10

In class:

Ch 6 Test

Lab Activity: continue Lab 6d part 2 – check for zone of inhibition

Lab Activity: Lab 6g Isolate HRP

HW: 

Read Lab 6g and 6 h Isolating HRP and Testing HRP using TMB

Finish Lab 6d New Products Lab Report – due on Tues 2/15

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 01/31/2022Date Due: 01/31/2022

M/W P1, P3, P5, P7

T/Th P2, P4, P6, P7 (Open House)

F P1, P3, P5, P7

Day 1 Tuesday, 2/1

In class:

Review Ch 6.3 and 6.4 Western Blot and go over ELISA Lab

Group presentation – Ch 6.5 Looking for new products

Due today: Lab 6c Amylase Lab

Lab Activity – Lab 6d Looking for new products, continue on Thurs

HW: 

Read Ch 6.6 Producing rDNA and do review questions

Finish ELISA Lab – due on Thurs 2/3

Day 2 Thursday, 2/3

In class:

Review Lab 6e Looking for new products

Due today: ELISA Lab

Group Presentation – Ch 6.6 Producing rDNA and correct review questions

Lab Activity: continue Lab 6d part 2 - Test plant extract against E.coli

HW: 

Do Biotech Live Activity 6.1-6.4 – due on Thurs 2/10

Lab 6d Looking for new products – due on Thurs 2/10

Ch 6 Test – Thurs 2/10; Ch 6 review questions-  due on Thurs 2/10

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 01/24/2022Date Due: 01/24/2022

M/T P1, P3, P5, P7

W Banking Day P1-P7

Th/F P2, P4, P6, P7

Day 1 Tuesday, 1/25

In class:

Review Ch 6.3 ELISA and Amylase

Finish Lab 6c Amylase Lab

Due today: Lab 6b Assay for Starch and Sugar

HW: 

Read ELISA Lab – Lab on Thursday

Read Ch 6.4 Western Blots and do review questions

Day 2 Thursday, 1/27

In class:

Review Lab 6c Amylase

Group Presentation – Ch 6.4 Western Blot 

Lab Activity: Anti-Heparin ELISA Assay

HW: 

Read Ch 6.5 Looking for new products

Do B.O. pg 178 Amazon Hide and Seek

Lab 6c Assaying for Amylase Activity – due Tues 2/1

Continue working on Synthetic Biology Project

Bring Herbs for Tuesday and read Lab 6d Looking for Potential Medicine 

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 01/17/2022Date Due: 01/17/2022

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Day 1 Tuesday, 1/18

In class:

Review Ch 6.1 Potential Biotech Product

Group 1 Presentation – Ch 6.2 The use of Assays, correct review questions

Due today: PAGE gel report

Lab Activity: Lab 6b Assay for Starch and Sugar

HW: 

Read Ch 6.3 ELISA and do review questions 6.3

Do Biotech Online ELISA Technology in Diagnostic Kits pg 173

Due on Thurs – Rapid ELISA, iFOBT

Day 2 Thursday, 1/20

In class:

Review Ch 6.2 Assays 

Group 1 Presentation - Ch 6.3 ELISA, correct review questions

Due today: Rapid ELISA, iFOBT report

Lab Activity: Lab 6c Assaying for Amylase Activity

HW: 

Read ELISA Lab

Finish Lab 6c Amylase Activity – due Thurs 1/27

Edpuzzle – ELISA

Continue working on Synthetic Biology Project

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 01/10/2022Date Due: 01/10/2022

M/Th P1, P3, P5, P7

W P1-P7 Banking Day

T/F P2, P4, P6, P7

Day 1 Tuesday, Jan 11

In class:

Review Ch 5 Proteins and PAGE, Polyacrylamide Gel Electrophoresis – lab report due on Tues, 1/18

Go over Synthetic Biology Project –due in 10 weeks 

Assign chapters for group presentations

HW: 

Read Ch 6.1 Assays

Do review questions 6.1

Brainstorm ideas for Synthetic Biology Project

Day 2 Thursday, Jan 13

In class:

Go over Synthetic Biology – go to www.Biobuilder.org 

Ch 6.1 Potential Products

Lab Activity: Rapid ELISA antibody assay

HW: 

Read Ch 6.2 Assays

Do review questions 6.2

Finish PAGE Report, due Tues 1/18

Chapter Assignments: Please prepare a powerpoint presentation for each section.

Grp1 - Ch 6.1-6.3

Grp2 – Ch 6.4-6.6

Grp3 – Ch 7.1, 7.4, 7.5

Grp4 – Ch 7.2, 7.3

Grp6 – Ch 8.1-8.5

Grp7 – Ch 9

Grp8 – Ch 12

Grp9 – Ch 13

Date Assigned: 12/13/2021Date Due: 12/13/2021

Monday P1, P3, P5, P7 – regular schedule

Tuesday P1-P7 Final Exam in P7 only

Wednesday P1, P3, P5 Final Exam

Thursday P2, P4, P6 Final Exam

Friday No School Winter Break Begins

Day 1 Monday, Dec 13 P7 2:39 pm - 3:29 pm

In class:

Review for Finals

HW:

Study for Finals, Units 1-4 – Finals on Thursday

Finish Study Guide for Finals

Day 2 Tuesday, Dec 14 P7 1:33 pm – 3:29 pm

Final Exam – Chapters 1-5

Enjoy your Winter Break!  

Date Assigned: 12/06/2021Date Due: 12/06/2021

M/Th P1, P3, P5, P7

W –  Banking Day, P1-P7

T/F P2, P4, P6, P7

Tuesday, Dec 7

In class:

Due today -– Antibody-Antigen Flu Test 

Go over Catalase Lab – Lab report due Thurs 12/9

Lab Activity: PAGE – Polyacrylamide Gel Electrophoresis

Correct Ch 5.4 Protein Analysis

Homework:

Study for Ch 5 Test – Test on Thurs 12/9

Complete Catalase Lab – due Thurs, 12/9

Thursday, Dec 9

In class:

Finish PAGE – Polyacrylamide Gel Electrophoresis

Due today – Catalase Lab report

Ch 5 Test

Homework:

Finish PAGE Lab report – due Tues, 12/14

Study for Finals, Ch 1-5 – Finals on Tues 12/14

Date Assigned: 11/29/2021Date Due: 11/29/2021

M/Th P1, P3, P5, P7

W –  Banking Day, P1-P7

T/F P2, P4, P6, P7

Tuesday, Nov 30

In class:

Review Ch 5 Protein Enzymes and correct assignments

Due today -– ABE Lab 6 and ABE Lab 7 Isolation of RFP by Column Chromatography

Lab Activity – Antibody-Antigen Test 

Homework:

Read Ch 5.1-5.3 more on Proteins

Complete Antibody-Antigen Test lab report – due Tues, 12/6

Thursday, Dec 2

In class:

Review Enzymes and Studying Proteins 

Lab Activity – Catalase Lab, parts A-D

Homework:

Graph results of Catalase Lab

Read PAGE Lab (Polyacrylamide Gel Electrophoresis)

Review Ch 5.4 and Study for Ch 5 Test

Ch 5 Test – Thurs, 12/9

Date Assigned: 11/22/2021Date Due: 11/22/2021

Reminder: Complete ABE Lab 6 Overnight Culture and Lab 7 Isolation of MFP by Column Chromatography – due on 11/30

Happy Thanksgiving!

Date Assigned: 11/15/2021Date Due: 11/15/2021

M/Th P1, P3, P5, P7

W –  Banking Day, P1-P7

T/F P2, P4, P6, P7

Tuesday, Nov 16

In class:

Presentations on Genetic Disorders

Ch 5.1 Protein Structure and Function

Lab Activity today – ABE Lab 6 Set up Overnight Culture of Transformed Cells

Quiz on Ch 5.1 Proteins 

Homework:

ABE ABE Lab 4, 5 Confirmation, Transformation due Thursday, 11/18

Read Ch 5.3 Protein Catalysts and do review questions

B.O. Catalysts for Better Health pg 151

Read ABE Lab 7 Overnight Cultures of Transformed E.coli; do prelab questions

Thursday, Nov 18

In class:

Go over ABE Lab 7 Overnight Cultures of Transformed E.coli

Ch 5.2 and 5.3 Protein Production and Protein Activity; correct review questions

Lab Activity: ABE Lab 7 Overnight Cultures of Transformed E.coli

Due today: ABE Lab 4 and 5

Homework:

Finish ABE Lab 6 and 7 Isolation of RFP by Column Chromatography – due on Tue 11/30

Read Ch 5.4 Studying Proteins and do review questions

Date Assigned: 11/08/2021Date Due: 11/08/2021

M/W       P1, P3, P5, P7

Thursday – Veteran's Day, No School

T/F           P2, P4, P6, P7

Tuesday, Nov 9

In class:

Review ABE Lab 4 and 5 – Co Confirmation of Digest and Transformation

Lab Activity today – ABE Lab 6 Set up Overnight Culture of Transformed Cells; Screen for Transformed, red cells

Quiz on ABE Lab 4, 5 Confirmation, and Transformation

Due today- Ch 4 Packet

Homework:

ABE ABE Lab 4, 5 Ligation, Transformation due Thursday, 11/8

Read Ch 5.1 Protein structure and function and do review questions

B.O. Antibody Producing Companies

Read ABE Lab 6 Overnight Cultures of Transformed E.coli; do prelab questions

Wednesday, Nov 10

In class:

Go over ABE Lab 6 Overnight Culture

Ch 5.2 Protein Production and Protein Activity

Homework:

Read ABE Lab 7 Isolation of RFP by Column Chromatography

Read Ch 5.2 Protein Production

B.O. Couch Potatoes pg 146

Date Assigned: 11/01/2021Date Due: 11/01/2021

T/Th P1, P3, P5, P7

W/F P2, P4, P6, P7

Tuesday, 11/2

In class:

Review ABE Lab 2 and 3 – Restriction Digest of pAra and pKan-R and Ligation

Lab Activity today – ABE Lab 4 Confirmation of Digest

Ch 4 Biotech Live – do Activities 4.1-4.3

ABE Labs 2 and 3 lab reports due on Thursday, 11/4

Quiz on ABE Lab 1 and 2

Homework:

Read ABE Lab 5 Transformation of E.coli with pAra-R

Complete ABE Lab 3 Ligation – Due on Tues, 11/9

Complete Ch 4 Biotech Live Activities 4.1-4.3

Thursday, Nov 4

In class:

Go over ABE Lab 4 Confirmation of Digest of pAra and pKan-R and Ligation

ABE Lab 5 Transformation

Complete Ch 4 Biotech Live Activities 4.4-4.6

Due today: ABE Labs 2 and 3 lab reports

Homework:

Read ABE Lab 6 Overnight Culture of Transformed E.coli

Finish ABE Labs 4 report – due Thurs 11/11

Complete Ch 4 Biotech Live Activities 4.4-4.6

Ch 4 packet due on Tue 11/9

Date Assigned: 10/25/2021Date Due: 10/25/2021

M/Th P1, P3, P5, P7

W P1-P7 Banking Day

T/F P2, P4, P6, P7

Monday, Oct 25

In class:

Go over Gel Electrophoresis

Correct 4.3-4.4 review questions; Ch 4 Test on Friday, 10/29

Set up ABE Lab 2 Restriction Digest of pAra and pKan

Due today: Gel Electrophoresis and Restriction Enzyme Activity, 

ABE Lab 1 Gel Electrophoresis – due on Thurs, 10/26

Homework:

Read ABE Lab 2 and 3

Do Biotech Live 4.1-4.6

Study for Ch 4 Test – Test on Fri, 10/29

Friday, Oct 29

In class:

Ch 4 Test

Go over ABE Lab 2 and set up ABE Lab 3 Ligation

Homework:

Read ABE Lab 4 Confirmation of Restriction Digest

Finish ABE Labs 2 and 3 report – due Thurs 11/5

Date Assigned: 10/18/2021Date Due: 10/18/2021

M/Th   P1, P3, P5, P7

W        P1-P7 Banking Day

T/F      P2, P4, P6, P7

Tuesday, Oct 19

In class:

Go over Math Problems and Ch 4.1 - 4.3 DNA Structure and Isolation of DNA

Quiz on Ch 4.1-4.3

Lab Activity: ABE Lab Gel Electrophoresis

Due: Strawberry DNA Isolation Lab

Homework:

Read Ch 4.4 Gel Electrophoresis and do review Questions

Strawberry DNA Lab – due on Tuesday

Thursday, Oct 21

In class:

Go over Ch 4.4 Gel Electrophoresis

Quiz on Gel Electrophoresis

Homework:

Do Biotech Online Chop and Go pg 124

Finish ABE Lab 1 Gel Electrophoresis – due on Tuesday 10/26

Read Ch 4 Summary and Lab Practices pgs 126-127

Ch 4 Test on Thursday, 10/28

Date Assigned: 10/11/2021Date Due: 10/11/2021

M/Th P1, P3, P5, P7

W PSAT 8:30-12;30

T/F P2, P4, P6, P7

Tuesday, Oct 12

In class:

Go over Math Problems

Ch 4.1 DNA Structure

Lab Activity: Isolation of Strawberry DNA 

Due today: Ch 3 Review questions and Math Problems

Homework:

Read Ch 4.2 Sources of DNA, do review questions

Do Biotech Online Know your Genome pg 112, Baldness Gene pg 118

Download Amgen Lab Manual

Strawberry DNA Lab – due on Tuesday

Thursday, Oct 14

In class:

Ch 4.2 Sources of DNA, correct review questions

Activity: Cast a Gel for Gel Electrophoresis; make 1x SB buffer and 1% agarose gel

Homework:

Read Ch 4.3 Isolating and Manipulating DNA

Do Biotech Online Recombinant Pharmaceuticals and Therapies, pgs 118-119

Read Lab 1 Amgen Lab Manual

Strawberry DNA Lab – due on Tuesday

Date Assigned: 10/04/2021Date Due: 10/04/2021

M/Th P1, P3, P5, P7

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7

Tuesday, Oct 5

In class:

Correct Ch 3.6 review questions and Math Problems

Ch 3 Test on Thursday

Due today: Pipetting Lab and DNA Lab

Lab Activity: Biotech Live – Activity 3.1-3.4 pgs 95-97 (work on Chromebook)

Homework:

Study for Ch 3 Test – Test on Thursday

Work on Math problems/Find MW of compounds

Thursday, Oct 7

In class:

Ch 3 Test

Continue with Biotech Live – Activity 3.5-3.6 pgs 98-100

Go over Math/MW of compounds

Homework:

Read Ch 4.1 Studying DNA and do review questions

Read Lab 1 Amgen Lab Manual

Bring Strawberries for Tuesday

Date Assigned: 09/27/2021Date Due: 09/27/2021

M/Th P1, P3, P5, P7

W P1-P7 (Banking Day)

T/F P2, P4, P6, P7

Tuesday, Sept 28

In class:

Correct all Ch 3.1 – 3.2 review questions and math problems

Go over Pipetting Lab part 1

Lab Activity – Pipetting Accuracy part 2

Homework:

Read Ch 3.5 and do review questions

Pipetting Accuracy Lab parts 1 and 2 – due on Tuesday, 10/5

Work on Math problems

Thursday, Sept 30

In class:

Go over Ch 3.5 review questions and math problems

Go over Pipetting Accuracy

Lab Activity – Micropippetting Exercise with colored dyes

Homework:

Read Ch 3.6 and do review questions

Read Summary and Lab Practices pgs 92-93

Pipetting Accuracy Lab parts 1 and 2 - due Tuesday, 10/5

Ch 3 Test – Tuesday, 10/5

Finish Math Problems

Date Assigned: 09/20/2021
Date Due: 09/20/2021
M/Th P1, P3, P5, P7

W P1-P7 

T/F P2, P4, P6, P7

Tuesday, Sept 21

In class:

Correct all Ch 2.4 and 2.5review questions

Go over Bacteria Lab and Gram Stain

Ch 2 Test – today; Ch 2 packet due 

Lab Activity – Gram Stain and use microscope to view bacteria

Homework:

Read Ch 3.1-3.2  pgs 71-82 and do review questions

Do B.O. pg 74

Finish Bacteria Lab and Gram Stain Lab – due on Tuesday, 9/28

Thursday, Sept 23

In class:

Go over Ch 3.1-3.2 review questions and math problems

Go over Gram Stain Lab

Lab Activity – Pippeting 

Homework:

Read Cj 3.3-3.4 and do review questions

Do Math Problems

Grams Stain Lab – due on Monday, 9/28

Pipetting Lab due Tuesday, 9/29

Date Assigned: 09/13/2021Date Due: 09/13/2021

M/Th   P1, P3, P5, P7

W          P1-P7 Banking Day

T/F        P2, P4, P6, P7

Tuesday, Sept 14

In class:

Go over Organic Lab and complete Cheese Lab

Go over Microscope Lab

Correct Ch 2.1-2.3 review questions

Lab Activity – Bacteria Lab: collect bacteria, streak media plate, test against antibiotics

Homework:

Read Ch 2.4 New Biotechnology and do 2.4 review questions

Do B.O. pg 52 and 82

Finish Cheese Lab and Organic Lab -  both are due on Thursday, 9/14

Microscope Lab – due on Tuesday 9/14

Ch 2 Test on Tuesday

Thursday, Sept 16

In class:

Due today: Cheese Lab and Organic Lab

Correct Ch 2.4 review questions

Go over Bacteria Lab

Lab Activity: Gram Staining

Homework:

Finish Bacteria Lab – due on Tuesday

Microscope Lab – due on Tuesday 9/14

Read Ch 2 Summary and Lab Practices pg 81-86

Ch 2 Test on Tuesday

Date Assigned: 09/06/2021Date Due: 09/06/2021

Tuesday, Sept 7

In class:

Review Ch 1.3-1.6 and correct review answers

Discuss careers and Biotethics in Biotechnology- watch video on Biochemist

Make 2^nd Observation of Cheese Lab – after 5 days, make cheeseball using cheesecloth

Lab Activity: Organic Lab

Homework:

Read Summary and Lab Practices on pgs 30-31 to review Ch 1Test

Study for Ch 1 Test – Test on Thursday, Sept 9

Cheese Lab is due on Tuesday, Sept. 14

Read Ch 2.1, do review question and Label parts of Microscope (worksheet) due on Thursday 9/9

Remember to copy review questions and answer in complete sentences on a separate sheet

Thursday, Sept 9

In class:

Ch 1 Test in class on Quizziz

Go over Ch 2.1 Review Questions, Organic Lab, and Microscope worksheet

Lab Activity: Organic Microscope and do cells worksheet on structures and function

Homework:

Read Ch 2.2-2.3, do review questions; remember to copy questions and answer in complete sentences

Complete cell structure and function worksheet

Finish Cheese Lab – due on Tues, 9/14

Finish Organic Lab – due on Thur, 9/16

Date Assigned: 08/30/2021Date Due: 08/30/2021

Tuesday, August 31

In Class:

What is Scientific Method?

Correct Ch 1.3 review questions

Lab Activity: Cheese Lab

Bleach Lab due – send to intern to check for content/format/completion; see below

HW:

Read ED Ch. 1.4 and review questions pgs 19-23 ED

Extra Credit: make cheese at home; DIY on foodwishes.com

Thursday, Sept 2

In Class:

Cheese Lab - Make 1^st observation, record in your notebook

Discuss Careers in Biotech

Correct Ch 1.4 review questions

HW:

Read ED Ch 1.5-1.6 and do review questions pgs 23-29 ED

Do B.O. Hot Jobs pg 26 and Code of Business Conduct pg 29 ED

Read Summary and Lab Practices pgs 30-31

Please share your lab report with your intern to get it checked before turning it in to me.

Biotech Interns:

Rosalia Park - rpar7075@stu.gusd.net                           A-C  (Last names)

Iris Milanovich-Rooney - mil3828@stu.gusd.net        D-H      

Kalia Manayan - Kman4559@stu.gusd.net                   J-K

Claire Yang - cyan6089@stu.gusd.net                           L-M

Michelle Chung - mchu2590@stu.gusd.net                  P-S                      

Nathan Pang - npan1489@stu.gusd.net                        W-Y

Date Assigned: 08/23/2021Date Due: 08/23/2021

Day 1 Tuesday, August 24

In class:

What is Biotech?

Go over How to maintain a Scientific Notebook in Biotech

Lab Activity: Bleach Lab  pgs 19-21 TB

HW:

Read Ch 1.1-1.2 and do review questions Ch 1.1-1.2 in TB

Read Cheese Lab before Thursday and bring whole milk and buttermilk on Thursday

Day 2 Thursday, August 26

In class:

Make final observations for Bleach Lab, lab report due on Tues 8/31

Review How to Notebook

Lab Activity: Cheese Lab

HW:

Read Ch 1.3 pgs 14-18 and do review questions 1.3 TB

Do Biotech Online (B.O.) pg 18

Finish Bleach Lab report in Google Docs pgs 19-21 TB; lab report on Tuesday

Date Assigned: 08/18/2021Date Due: 08/18/2021

Week 1 Semester 1 August 18-20, 2021 Assignments in Biotech

Day 1 Wednesday, August 18

In class:

Rules and Policies

Safety Rules

HW:

Sign up for Google Classroom, Quizizz

Study for Safety Test – Test on Thursday

Wear shoes to class – Always!

Day 2 Thursday, August 19

In class:

Safety Test

What is Biotechnology

HW:

Read Ch 1.1 Biotechnology ED (Ellyn Daugherty textbook)

Read Bleach Lab pgs 19-21 ED, Lab on Tuesday

Wear shoes please

Date Assigned: 06/07/2021Date Due: 06/07/2021

Finals Week:   We will meet on T/Th at 1:30 pm

Tuesday, June 8

In class:

Finish Career Exploration Project – Oral Presentations

Final Exam – Part 1

HW:                 

Study for Finals, Ch 6-9 – Finals Part 2 on Thursday  

Thursday, June 10

In class:

Finals Part 2

HW:

Have a great summer!

Date Assigned: 05/31/2021Date Due: 05/31/2021

Tuesday, June 1

In class:

Career Exploration Project – Oral Presentations

HW:                 

Study for Finals – Finals on Tuesday, June 8^th and June 10^th

Thursday, June 3

In class:

Continue with Oral Presentations on Career Exploration

HW:

Study for Finals – Finals on Tuesday, June 8^th and June 10^th

Date Assigned: 05/24/2021Date Due: 05/24/2021

Tuesday, May 25

In class:

Resumes and Career Exploration Project – due today

Presentations on Career Exploration Project – begin today

HW:

Study for Finals – Finals on Tuesday, June 8th

Thursday, May 27

In class:

Continue with Oral Presentations on Career Exploration

HW:

Study for Finals – Finals on Tuesday, June 8th

Date Assigned: 05/17/2021Date Due: 05/17/2021

Tuesday, May 18

In class:

Ch 13.1 and 13.2 DNA Synthesis – Groups 1 and 2 presentations

Work on Career Exploration Project

HW:

Read Ch 13.3 Polymerase Chain Reaction and do review questions

EP: PCR

Thursday, May 20

In class:

Ch 13.3 PCR – Group 3 and 4 presentations

HW:

Read Ch 13.4 Applications of PCR and do review questions

B.O. – CSI – your room pg 383

EP: Karyotyping/DNA Fingerprinting

Date Assigned: 05/10/2021Date Due: 05/10/2021